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4495287 Process for producing gene products of plasmid having temperature dependent plasmid copy number
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PATENT ABSTRACTS
4492758 HYBRID VECTOR AND PROCESS FOR IMPROVING THE AMPLIFICATION AND EXPRESSION OF HYBRID VECTORS BY THE USE OF MITOCHONDRIAL DNA Karl Esser, Ulf Stahl, Paul Tudzynski, Ulrich Kuck, Bochum, Federal Republic Of Germany assigned to Hoechst AktiengeseHschaft What are disclosed are a hybrid vector synthesized from a segment of mitochondrial DNA containing an origin of replication and. optionally, from a segment of a prokaryotic plasmid, and a method for improving the amplification and expression of hybrid vectors in eukaryotic bost cells using mitochondrial DNA.
minator is desirably followed by a marker allowing for selection of transformants. High efficiencies in transcription of DNA can be achieved with the highly active T5 promoters. The promoters and terminators are used in hybrid DNA for efficient expression of structural genes and transcription to provide RNA sequences.
4495287 PROCESS FOR PRODUCING GENE PRODUCTS OF PLASMID HAVING TEMPERATURE DEPENDENT PLASMID COPY NUMBER Bernt E Uhlin, Kurt Nordstrom, Soeren Molin, Odense, Denmark assigned to A/S Alfred Benzon
4493893 PROCESS FOR CLONING THE GENE CODING FOR A THERMOSTABLE ALPHAAMYLASE INTO ESCHERICHIA COLI AND BACILLUS SUBTILIS
Gene products of plasmid DNA, such as proteins, are prepared in high yields by cultivating bacteria carrying a plasmid which shows a controlled constant plasmid copy number at one temperture and a much higher or totally uncontrolled copy number at a different temperaturc. The plasmid may be prepared by recombinant DNA technique using a cloning vector showing the temperature dependent plasmid copy number pattern.
Jonathan R Mielenz, Susan Mickel assigned to CPC International Inc
4496521 An improved process for producing a thermostable alpha-amylase enzyme is described. The gene coding for the alpha-amylase is incorporated into a chimeric plasmid which is produced in multiple copies by a host microorganism.
INSULIN-POTENTIATING PEPTIDES U James Lewis assigned to The Whittier Institute for Diabetes and Endocrinology hGH(1-43) having the formula: H-Phe-Pro-Thr-
lle-Pro-Leu-Ser-Arg-Leu-Phe-Asp-Asn-Ala4495280 CLONED HIGH SIGNAL STRENGTH PROMOTERS Hermann G Bujard, Annie C Y Chang, Stanley N Cohen, Heidelberg, CA, Federal Republic Of Germany assigned to The Board of Trustees of the Leland Stanford Jr University Method for preparing high signal strength promoters and terminators and DNA compositions employing such promoters and terminators. T5 phage is cleaved to provide for DNA sequences having intact promoters. These promoters are inserted into vectors separated from a balanced terminator by a gene of interest and the ter-
Met-Leu-Arg-Ala-His- Arg-Leu-His-Gln-LeuAla-Phe-Asp-Thr-Try-Gln-Glu-Phe-Glu-GluAla-Try-lle-Pr o-Lys-Glu-Gln-Lys-Tyr-Ser-OH has been found to have remarkable insulinpotentiating action, as has the fragment hGH (17-43). Pharmaceutical compositions in accordance with the invention include hGH(1-43), hGH(17-43) or a biologically active intermediate fragment, or a nontoxic salt thereof, dispersed in a pharmaceutically acceptable carrier. Such peptides or pharmaceutically acceptable salts thereof may be administered to mammals to promote the action ofinsulin. Such a peptide can be synthesized by exclusively solid-phase techniques, b.', partial solid-phase techniques, by fragment condensation, by classical solution addition or by recently developed recombinant DNA techniques.
PATENT ABSTRACTS
4492758 HYBRID VECTOR AND PROCESS FOR IMPROVING THE AMPLIFICATION AND EXPRESSION OF HYBRID VECTORS BY THE USE OF MITOCHONDRIAL DNA Karl Esser, Ulf Stahl, Paul Tudzynski, Ulrich Kuck, Bochum, Federal Republic Of Germany assigned to Hoechst AktiengeseHschaft What are disclosed are a hybrid vector synthesized from a segment of mitochondrial DNA containing an origin of replication and. optionally, from a segment of a prokaryotic plasmid, and a method for improving the amplification and expression of hybrid vectors in eukaryotic bost cells using mitochondrial DNA.
minator is desirably followed by a marker allowing for selection of transformants. High efficiencies in transcription of DNA can be achieved with the highly active T5 promoters. The promoters and terminators are used in hybrid DNA for efficient expression of structural genes and transcription to provide RNA sequences.
4495287 PROCESS FOR PRODUCING GENE PRODUCTS OF PLASMID HAVING TEMPERATURE DEPENDENT PLASMID COPY NUMBER Bernt E Uhlin, Kurt Nordstrom, Soeren Molin, Odense, Denmark assigned to A/S Alfred Benzon
4493893 PROCESS FOR CLONING THE GENE CODING FOR A THERMOSTABLE ALPHAAMYLASE INTO ESCHERICHIA COLI AND BACILLUS SUBTILIS
Gene products of plasmid DNA, such as proteins, are prepared in high yields by cultivating bacteria carrying a plasmid which shows a controlled constant plasmid copy number at one temperture and a much higher or totally uncontrolled copy number at a different temperaturc. The plasmid may be prepared by recombinant DNA technique using a cloning vector showing the temperature dependent plasmid copy number pattern.
Jonathan R Mielenz, Susan Mickel assigned to CPC International Inc
4496521 An improved process for producing a thermostable alpha-amylase enzyme is described. The gene coding for the alpha-amylase is incorporated into a chimeric plasmid which is produced in multiple copies by a host microorganism.
INSULIN-POTENTIATING PEPTIDES U James Lewis assigned to The Whittier Institute for Diabetes and Endocrinology hGH(1-43) having the formula: H-Phe-Pro-Thr-
lle-Pro-Leu-Ser-Arg-Leu-Phe-Asp-Asn-Ala4495280 CLONED HIGH SIGNAL STRENGTH PROMOTERS Hermann G Bujard, Annie C Y Chang, Stanley N Cohen, Heidelberg, CA, Federal Republic Of Germany assigned to The Board of Trustees of the Leland Stanford Jr University Method for preparing high signal strength promoters and terminators and DNA compositions employing such promoters and terminators. T5 phage is cleaved to provide for DNA sequences having intact promoters. These promoters are inserted into vectors separated from a balanced terminator by a gene of interest and the ter-
Met-Leu-Arg-Ala-His- Arg-Leu-His-Gln-LeuAla-Phe-Asp-Thr-Try-Gln-Glu-Phe-Glu-GluAla-Try-lle-Pr o-Lys-Glu-Gln-Lys-Tyr-Ser-OH has been found to have remarkable insulinpotentiating action, as has the fragment hGH (17-43). Pharmaceutical compositions in accordance with the invention include hGH(1-43), hGH(17-43) or a biologically active intermediate fragment, or a nontoxic salt thereof, dispersed in a pharmaceutically acceptable carrier. Such peptides or pharmaceutically acceptable salts thereof may be administered to mammals to promote the action ofinsulin. Such a peptide can be synthesized by exclusively solid-phase techniques, b.', partial solid-phase techniques, by fragment condensation, by classical solution addition or by recently developed recombinant DNA techniques.