lO0
PATENT ABSTRACTS
of the tube gels. D N A is employed as the substrate for casting the slab gels. The slab gels are electrophoresed for the secon...
of the tube gels. D N A is employed as the substrate for casting the slab gels. The slab gels are electrophoresed for the second dimension in a chamber wherein the rack containing a stack of slab gets forms the partition between the anolyte and catholyte compartments. After the seconddimension electrophoresis, the slab gels are incubated in incubation buffer and placed in Pyronin Y to stain the unhydrolized DNA. After staining, they are destained in acetic acid. The DNAses are then visible as colorless spots in a reddish-colored gel.
assay sample and a receptacle containing (b) an amoebocyte lysate component or horseshoe crab. The acid treatment of the assay sample results in improved accuracy, reliability and reproducibility of the bacterial endotoxin determination.
4496470 CLEANING
COMPOSITION
Anita G Kapilofl', Randolph T Hatch assigned to The B F Goodrich Company 4495289 TISSUE
CULTURE
CLUSTER
DISH George F Lyman, Alan Lowry assigned to Data Packaging Corporation
I~--io / r~ ~z A composition for cleaning fouled reverse osmosis membranes or other similar fouled surfaces. The composition will remove calcium and magnesium scale as well as iron scale and organic fouling materials. The composition is biodegradable and safe to use.
A tissue culture cluster dish having a multi-well base and detached cover. The cover may be mounted on the base in a partially open position to provide access to the wells by a pipette or other instrument operated by the user while the cover acts as a shield against airborne contaminants.
4495294
M E T H O D FOR DETERMINING BACTERIAL E N D O T O X I N AND KIT THEREFOR Chizuko Nakahara, Shigenori Tanaka, Hiroshi Tamura, Akiyosbi Matsumoto, Hino, Japan assigned to Seikagaku Kogyo Co Ltd Bacterial endotoxin in an assay sample is determined by an amoebocyte lysate component of horseshoe crab in the presence or absence of a substrate for endotoxin determination by using an assay sample in the liquid state obtained by treating said assay sample with an endotoxinfree acid having a pKa25 degrees C. value of not more than 3 at a pH of not more than 3; and a kit therefor, a receptacle containing (a) an endotoxin-free acid having a pKa25 degrees C. value of not more than 3 e.g. perchloric acid, ~richloroacetic acid, nitric acid, for treating the
4496658 METHOD FOR ENZYME IMMUNOASSAY AND PRODUCTION OF ANTIBODY Koichi Kondo, Susumu lwasa, lsam Yoshida, Osaka, Japan assigned to Takeda Chemical Industries Etd In a method for immunochemical assay of human chorionic gonadotropin involving use of an antibody immobilized on a carrier, an antigen and an antibody to which a labeling agent has been attached, when the antibody supported on the carrier and the antibody coupled to a labeling agent are different antibodies which are not overlapping in antigen-determining site and one of said different antibodies is specifically reactive to human chorionic gonadotropin, a high reproducibility of the result of the immunochemical assay is obtained. 4499014 METHOD FOR PURIFYING GAMMA-INTERFERON Leonard Estis assigned to Interferon Sciences lnc