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4496655 Process for the production of tyramine oxidase
PATENT ABSTRACTS
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4496655
COUPLED ENZYME SYSTEMS FOR DETERMINATION OF DISSOLVED SUBSTANCES
PROCESS FOR THE PRODUCTION OF TYRAMINE OXIDASE
Berthold Limbach, Roland Helger, Seeheim Jugenheim, Federal Republic Of Germany assigned to Merck Patent Gesellschaft mit beschrankter Haftung
Eiichi Yoshino, Kazuo Matsuura, Hideo Misaki, Shizuoka, Japan assigned to Toyo Jozo Kabushiki Kaisha
A test system having an extended range of measurement and a corresponding procedure for the determination of substances in liquids contains several enzymes or enzyme systems together which, independently of one another, react directly or indirectly with the substance to be determined. On adding the test system to the sample solution, various final products are produced which can be distinguished analytically and evaluated by measurement techniques or visually. For example, glucose can be determined over a wide range of concentrations using a combination of an NAD-dependent dehydrogenase and a non-NAD-dependent dehydrogenase.
Tyramine oxidase, which is useful for the clinical diagnosis of leucine aminopeptidase activity in serum, is produced by culturing the microorganism Arthrobacter sp. B-0813 FERM-P No. 6240 in a nutrient medium, and isolating thus-produced tyramine oxidase from the cultured medium.
4496656 PROCESS FOR PRODUCTION CELLULASE
OF
Katsumi Nakamura, Kumpei Kitamura, Takasaki, Japan assigned to Kirin Beer Kabushiki Kaisha
4495296 LABELED ANTI-HAPTEN ANTIBODIES AND THEIR USE AS A UNIVERSAL REAGENT FOR SOLID PHASE RADIO- AND]OR ENZYME-IMMUNOASSAYS A Robert Neurath, Nathan Strick assigned to New York Blood Center Inc A process for detecting the presence of an antigen in a specimen is described, which process comprises: (A) contacting said specimen with a substrate coated with antibodies of said antigen, incubating the contacted substrate and washing the substrate; (B) contacting the washed material of step (A) with a hapten conjugated antibody against said antigen, incubating the so-contacted material and washing the so-incubated material; (C) contacting the washed material of step (B) with a radioactive material labeled or enzyme containing anti-hapten antibody, incubating the so-contacted material and washing the same; and (D) effecting radioimmunoassay if said antibody is radioactive or enzyme labeled immunoassay if said antibody contains an enzyme moiety. Quantitative determination of the antigen in the specimen is effected by comparing the counts of the radioimmunoassay or the concentration of enzyme against a standard as by photocolormetric methods.
A novel bacterium, Cellulomonas uda CB4, and production of cellulase by cultivation of the bacterium on a medium containing a cellulosic material are disclosed.
4496658 METHOD FOR ENZYME IMMUNOASSAY AND PRODUCTION OF ANTIBODY Koichi Kondo, Susumu lwasa, Isam Yoshida, Osaka, Japan assigned to Takeda Chemical Industries Ltd In a method for immunochemical assay of human chorionic gonadotropin involving use of an antibody immobilized on a carrier, an antigen and an antibody to which a labeling agent has been attached, when the antibody supported on the carrier and the antibody coupled to a labeling agent are different antibodies which are not overlapping in antigen-determining site and one of said different antibodies is specifically reactive to human chorionic gonadotropin, a high reproducibility of the result of the immunochemical assay is obtained.
107
4490465
4496655
COUPLED ENZYME SYSTEMS FOR DETERMINATION OF DISSOLVED SUBSTANCES
PROCESS FOR THE PRODUCTION OF TYRAMINE OXIDASE
Berthold Limbach, Roland Helger, Seeheim Jugenheim, Federal Republic Of Germany assigned to Merck Patent Gesellschaft mit beschrankter Haftung
Eiichi Yoshino, Kazuo Matsuura, Hideo Misaki, Shizuoka, Japan assigned to Toyo Jozo Kabushiki Kaisha
A test system having an extended range of measurement and a corresponding procedure for the determination of substances in liquids contains several enzymes or enzyme systems together which, independently of one another, react directly or indirectly with the substance to be determined. On adding the test system to the sample solution, various final products are produced which can be distinguished analytically and evaluated by measurement techniques or visually. For example, glucose can be determined over a wide range of concentrations using a combination of an NAD-dependent dehydrogenase and a non-NAD-dependent dehydrogenase.
Tyramine oxidase, which is useful for the clinical diagnosis of leucine aminopeptidase activity in serum, is produced by culturing the microorganism Arthrobacter sp. B-0813 FERM-P No. 6240 in a nutrient medium, and isolating thus-produced tyramine oxidase from the cultured medium.
4496656 PROCESS FOR PRODUCTION CELLULASE
OF
Katsumi Nakamura, Kumpei Kitamura, Takasaki, Japan assigned to Kirin Beer Kabushiki Kaisha
4495296 LABELED ANTI-HAPTEN ANTIBODIES AND THEIR USE AS A UNIVERSAL REAGENT FOR SOLID PHASE RADIO- AND]OR ENZYME-IMMUNOASSAYS A Robert Neurath, Nathan Strick assigned to New York Blood Center Inc A process for detecting the presence of an antigen in a specimen is described, which process comprises: (A) contacting said specimen with a substrate coated with antibodies of said antigen, incubating the contacted substrate and washing the substrate; (B) contacting the washed material of step (A) with a hapten conjugated antibody against said antigen, incubating the so-contacted material and washing the so-incubated material; (C) contacting the washed material of step (B) with a radioactive material labeled or enzyme containing anti-hapten antibody, incubating the so-contacted material and washing the same; and (D) effecting radioimmunoassay if said antibody is radioactive or enzyme labeled immunoassay if said antibody contains an enzyme moiety. Quantitative determination of the antigen in the specimen is effected by comparing the counts of the radioimmunoassay or the concentration of enzyme against a standard as by photocolormetric methods.
A novel bacterium, Cellulomonas uda CB4, and production of cellulase by cultivation of the bacterium on a medium containing a cellulosic material are disclosed.
4496658 METHOD FOR ENZYME IMMUNOASSAY AND PRODUCTION OF ANTIBODY Koichi Kondo, Susumu lwasa, Isam Yoshida, Osaka, Japan assigned to Takeda Chemical Industries Ltd In a method for immunochemical assay of human chorionic gonadotropin involving use of an antibody immobilized on a carrier, an antigen and an antibody to which a labeling agent has been attached, when the antibody supported on the carrier and the antibody coupled to a labeling agent are different antibodies which are not overlapping in antigen-determining site and one of said different antibodies is specifically reactive to human chorionic gonadotropin, a high reproducibility of the result of the immunochemical assay is obtained.