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4497796 Gene transfer in intact mammals
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PATENT ABSTRACTS
4497796 GENE TRANSFER IN INTACT MAMMALS
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Winston A Salser, Martin Cline, Howard Stang assigned to The Regents of the University of California Methods and compositions are provided for gene transfer to intact mammals with expression of the exogenous genetic material in the host. Mammalian host cells which are regenerative, normally highly proliferative or subject to induced proliferation, are transformed or modified in vitro with DNA capable of replication and expression in the host cell, wherein the DNA becomes incorporated into the cell. The modified cells are found to regenerate in the host with expression of the introduced DNA. Particularly, mammalian cells were modified with genes providing for overproduction of a particular enzyme. The modified cells were reintroduced in the host under conditions providing for selective advantage of the modified cells.
4499183 DETECTING INTRACELLULAR ANTIGENS IN SWELLED AND FIXED CELLS WITH LABELED ANTIBODY Daniel J Sujansky, Martha W Rancourt assigned to Ortho Diagnostic Systems lnc Methods and reagent kits for the detection of intracellular antigens such as TdT and antinuclear antibodies. The methods involve swelling the cells with a hypotonic solution for increasing the localization of large molecular weight substances such as antibodies at intracellular antigenic sites, fixing the cells and reacting them with antibodies specific for the intracellular antigen to be detected. The cells are then further reacted with labeled antibody specific for the first antibody, washed to remove unreacted antibodies and the presence of label detected whereby the presence or absence of the intracellular antigen may be determined.
4499188 BACTERIAL PRODUCTION OF HETEROLOGOUS POLYPEPTIDES UNDER THE CONTROL OF A REPRESSIBLE PROMOTER-OPERATOR Michael W Konrad, David Mark assigned to Cetus Corporation
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A process for bacterially producing beterologous polypeptides, particularly those such as human IFN- beta that inhibit bacterial growth, in which bacteria that have been transformed to express the heterologous polypeptide under the control of a trp promoter-operator are cultivated in a known volume of medium containing an excess of a preferred carbon source such as glucose and a predetermined amount of tryptophan that corresponds approximately to the amount of tryptophan contained in the bacteria in the volume of medium at a predetermined elevated cellular density, whereby expression of the heterologous polypeptide is substantially repressed until the bacteria grow to approximately the predetermined elevated cellular density and is thereafter automatically derepressed to permit expression of the heterologous polypeptide.
4499189 BACTERIA CARRYING PLASMID HAVING TEMPERATURE DEPENDENT PLASMID COPY NUMBER Bernt E Uhlin, Kur Nordstrom, Soeren Molin, Odense, Denmark assigned to A/S Alfred Benzon Gene products of plasmid DNA, such as proteins, are prepared in high yields by cultivating bacteria carrying a plasmid which shows a controlled constant plasmid copy number at one temperature and a much higher or totally uncontrolled copy number at a different temperature. The plasmid may be prepared by recombinant DNA technique using a cloning vector showing the temperature dependent plasmid copy number pattern.
4500640 PLASMIDS Ryoich Katsumata, Tetsuo Oka, Akira Furuya, Machida, Japan assigned to Kyowa Hakko Kogyo Co Ltd
PATENT ABSTRACTS
4497796 GENE TRANSFER IN INTACT MAMMALS
-
/
:
!
Winston A Salser, Martin Cline, Howard Stang assigned to The Regents of the University of California Methods and compositions are provided for gene transfer to intact mammals with expression of the exogenous genetic material in the host. Mammalian host cells which are regenerative, normally highly proliferative or subject to induced proliferation, are transformed or modified in vitro with DNA capable of replication and expression in the host cell, wherein the DNA becomes incorporated into the cell. The modified cells are found to regenerate in the host with expression of the introduced DNA. Particularly, mammalian cells were modified with genes providing for overproduction of a particular enzyme. The modified cells were reintroduced in the host under conditions providing for selective advantage of the modified cells.
4499183 DETECTING INTRACELLULAR ANTIGENS IN SWELLED AND FIXED CELLS WITH LABELED ANTIBODY Daniel J Sujansky, Martha W Rancourt assigned to Ortho Diagnostic Systems lnc Methods and reagent kits for the detection of intracellular antigens such as TdT and antinuclear antibodies. The methods involve swelling the cells with a hypotonic solution for increasing the localization of large molecular weight substances such as antibodies at intracellular antigenic sites, fixing the cells and reacting them with antibodies specific for the intracellular antigen to be detected. The cells are then further reacted with labeled antibody specific for the first antibody, washed to remove unreacted antibodies and the presence of label detected whereby the presence or absence of the intracellular antigen may be determined.
4499188 BACTERIAL PRODUCTION OF HETEROLOGOUS POLYPEPTIDES UNDER THE CONTROL OF A REPRESSIBLE PROMOTER-OPERATOR Michael W Konrad, David Mark assigned to Cetus Corporation
':
1
)
tr~t ~r l
A process for bacterially producing beterologous polypeptides, particularly those such as human IFN- beta that inhibit bacterial growth, in which bacteria that have been transformed to express the heterologous polypeptide under the control of a trp promoter-operator are cultivated in a known volume of medium containing an excess of a preferred carbon source such as glucose and a predetermined amount of tryptophan that corresponds approximately to the amount of tryptophan contained in the bacteria in the volume of medium at a predetermined elevated cellular density, whereby expression of the heterologous polypeptide is substantially repressed until the bacteria grow to approximately the predetermined elevated cellular density and is thereafter automatically derepressed to permit expression of the heterologous polypeptide.
4499189 BACTERIA CARRYING PLASMID HAVING TEMPERATURE DEPENDENT PLASMID COPY NUMBER Bernt E Uhlin, Kur Nordstrom, Soeren Molin, Odense, Denmark assigned to A/S Alfred Benzon Gene products of plasmid DNA, such as proteins, are prepared in high yields by cultivating bacteria carrying a plasmid which shows a controlled constant plasmid copy number at one temperature and a much higher or totally uncontrolled copy number at a different temperature. The plasmid may be prepared by recombinant DNA technique using a cloning vector showing the temperature dependent plasmid copy number pattern.
4500640 PLASMIDS Ryoich Katsumata, Tetsuo Oka, Akira Furuya, Machida, Japan assigned to Kyowa Hakko Kogyo Co Ltd