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4517288 Solid phase system for ligand assay
PATENT ABSTRACTS
246
zyme. at least one buffer substance and at least one indicator substance and optionally contains adjuvants, wherein the reagent contains a definite amount of the enzyme substrate in an insufficient amount with regard to the substrate determination capacity of the system for the determination of this substrate. The present invention also provides a process for the enzymatic determination of an enzyme substrate in the presence of disturbing substances which can react with the substrate itself or with an intermediate or end product of the indicator reaction. wherein, before the addition of the sample to be investigated, a definite amount of the substrate is first reacted with the reagent until reaction is complete, whereafter the sample to be determined is added.
mula: See Patent f o r Tabular Presentation PS wherein R 1 is a peptide fragment consisting of I to 14 amino acid residues including Gly in the 14position of the peptide Alal-Pro2-Pro3-Pro4Ser5-Leu6-Pro7-Ser8-Pro9-Ser 10-Argl ILeul2-Prol3-Glyl4 is used, a high reproducibility of the result of the enzyme immunoassay is obtained.
4517288
Samue Schatkowsky, William Pepper assigned to Spiral System Instruments Inc
SOLID PHASE SYSTEM LIGAND ASSAY
4517292 METHOD AND APPARATUS FOR TESTING MICROBIAL INTERACTION WITH GROWTH AFFECTING SUBSTANCES
FOR
Joseph L Giegel, Mary M Brotherton assigned to American Hospital Supply Corp A method for conducting a ligand assay in an inert porous medium wherein a binding material is immunologically immobilized within the medium, which includes the steps of immunologically immobilizing a binding material within a finite zone of the medium, applying an analyte to the zone containing the immobilized binding material, applying a labeled indicator to the zone which becomes immobilized within the zone in an amount which can be correlated to the amount of analyte in the zone, applying a solvent to substantially the center of the zone to chromatographically separate the unbound labeled indicator from the zone. and measuring the amount of labeled indicator remaining in the zone.
4517290 METHOD FOR ENZYME IMMUNOASSAY AND PEPT1DEENZYME CONJUGATE AND ANTIBODY THEREFOR Susum lwasa, Isamu Yoshida, Koichi Kondo. Tsuzuki. Japan assigned to Takeda Chemical Industries Ltd In an enzyme immunoassay, when a specific antibody produced by contacting a peptide essential to the formation of a specific antibody to a peptide antigen, a freeze-dried material of betaD-galactosidase-enzyme conjugate or a peptideenzyme conjugate prepared by coupling a labeling enzyme with a peptide of the general for-
A method and apparatus for calculating the growth interacting substance potency and growth ratio for the intersections of a scanning spot with a track of visible microbial colonies on an interaction plate. The potency is a measure of the weight of a growth interacting substance which is deposited on the interaction plate per unit volume of the growth culture medium. The growth ratio is a measure of the growth interacting substance effect on growth of colonies of microbes on the interaction plate along the path of interaction of the scanning spot with visible microbial colonies.
4517338 MULTIPLE REACTOR SYSTEM AND METHOD FOR POLYNUCLEOTIDE SYNTHESIS Mickey S Urdea, Brian D Warner assigned to Chiron Corporation A reactor system and method for synthesizing or degrading polynucleotides and other linear polymers includes a tubular reactor connected to a reagent manifold. The polynucleotide is immobilized on a loosely packed solid-phase support material in the tubular reactor, and reagents are sequentially introduced into the tubular reactor. After each reagent is introduced, the tubular reactor is isolated from the reagent manifold and the reagent agitated by alternately pressurizing the opposite ends of the tubular reactor. The method provides rapid and efficient synthesis of polynucleotides. By connecting two or more tubular reactors to the reagent manifold, a plurality of polynucleotides having different sequences may be synthesized simultaneously.
246
zyme. at least one buffer substance and at least one indicator substance and optionally contains adjuvants, wherein the reagent contains a definite amount of the enzyme substrate in an insufficient amount with regard to the substrate determination capacity of the system for the determination of this substrate. The present invention also provides a process for the enzymatic determination of an enzyme substrate in the presence of disturbing substances which can react with the substrate itself or with an intermediate or end product of the indicator reaction. wherein, before the addition of the sample to be investigated, a definite amount of the substrate is first reacted with the reagent until reaction is complete, whereafter the sample to be determined is added.
mula: See Patent f o r Tabular Presentation PS wherein R 1 is a peptide fragment consisting of I to 14 amino acid residues including Gly in the 14position of the peptide Alal-Pro2-Pro3-Pro4Ser5-Leu6-Pro7-Ser8-Pro9-Ser 10-Argl ILeul2-Prol3-Glyl4 is used, a high reproducibility of the result of the enzyme immunoassay is obtained.
4517288
Samue Schatkowsky, William Pepper assigned to Spiral System Instruments Inc
SOLID PHASE SYSTEM LIGAND ASSAY
4517292 METHOD AND APPARATUS FOR TESTING MICROBIAL INTERACTION WITH GROWTH AFFECTING SUBSTANCES
FOR
Joseph L Giegel, Mary M Brotherton assigned to American Hospital Supply Corp A method for conducting a ligand assay in an inert porous medium wherein a binding material is immunologically immobilized within the medium, which includes the steps of immunologically immobilizing a binding material within a finite zone of the medium, applying an analyte to the zone containing the immobilized binding material, applying a labeled indicator to the zone which becomes immobilized within the zone in an amount which can be correlated to the amount of analyte in the zone, applying a solvent to substantially the center of the zone to chromatographically separate the unbound labeled indicator from the zone. and measuring the amount of labeled indicator remaining in the zone.
4517290 METHOD FOR ENZYME IMMUNOASSAY AND PEPT1DEENZYME CONJUGATE AND ANTIBODY THEREFOR Susum lwasa, Isamu Yoshida, Koichi Kondo. Tsuzuki. Japan assigned to Takeda Chemical Industries Ltd In an enzyme immunoassay, when a specific antibody produced by contacting a peptide essential to the formation of a specific antibody to a peptide antigen, a freeze-dried material of betaD-galactosidase-enzyme conjugate or a peptideenzyme conjugate prepared by coupling a labeling enzyme with a peptide of the general for-
A method and apparatus for calculating the growth interacting substance potency and growth ratio for the intersections of a scanning spot with a track of visible microbial colonies on an interaction plate. The potency is a measure of the weight of a growth interacting substance which is deposited on the interaction plate per unit volume of the growth culture medium. The growth ratio is a measure of the growth interacting substance effect on growth of colonies of microbes on the interaction plate along the path of interaction of the scanning spot with visible microbial colonies.
4517338 MULTIPLE REACTOR SYSTEM AND METHOD FOR POLYNUCLEOTIDE SYNTHESIS Mickey S Urdea, Brian D Warner assigned to Chiron Corporation A reactor system and method for synthesizing or degrading polynucleotides and other linear polymers includes a tubular reactor connected to a reagent manifold. The polynucleotide is immobilized on a loosely packed solid-phase support material in the tubular reactor, and reagents are sequentially introduced into the tubular reactor. After each reagent is introduced, the tubular reactor is isolated from the reagent manifold and the reagent agitated by alternately pressurizing the opposite ends of the tubular reactor. The method provides rapid and efficient synthesis of polynucleotides. By connecting two or more tubular reactors to the reagent manifold, a plurality of polynucleotides having different sequences may be synthesized simultaneously.