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4532089 Method of preparing giant size liposomes
Healthcare Products
4530787
using these molecules, hosts, genes and polypeptides. The recombinant DNA molecules are characterized by structural genes that code for a polypeptide displaying a biological or immulogical activity of human interferon. In appropriate hosts these molecules permit the production and identification of genes and polypeptides displaying a biological or immunological activity of human interferon and their use in antiviral and antitumor or anticancer agents.
CONTROLLED OXIDATION OF MICROBIALLY PRODUCED CYSTEINE-CONTAINING PROTEINS Ze'e Shaked, Sidney Wolfe assigned to Cetus Corporation Method of oxidizing reduced cysteinecontaining microbially produced synthetic proteins, such as synthetic IFN- beta or synthetic IL-2, in a controlled manner so that the synthetic proteins have the same disulfide bridging as their native counterparts. The oxidation employs oiodosobenzoate as oxidizing agent and is carried out in an aqueous medium at a pH at least about one-half pH unit less than the pKa of the cysteines to be oxidized, a synthetic protein concentration of less than about 5 mg/ml, and an oxidizing agent:protein tool ratio that is at least stoichiometric, provided that the oxidizing agent is in excess in the terminal portion of the reaction.
4530908 DIAGNOSIS AND TREATMENT OF FLUKE INFECTIONS WITH MONOCLONAL ANTIBODIES Mette Strand assigned to The Johns Hopkins University Antibodies and antigens are disclosed which provide a method of detecting and a method of combating flukes. A diagnostic method for the determination of an active fluke infection in a warm-blooded animal is provided which comprises the testing of body serum or body fluids for the presence of fluke spine glycoprotein or anti-spine antibodies. Fused cell hybrids ATCC HB-8086, ATCC HB-8087 and ATCC HB-8088, as well as the antibodies produced by the fused cell hybrids, are also provided.
4530901 RECOMBINANT DNA MOLECULES AND THEIR USE IN PRODUCING HUMAN INTERFERON-LIKE POLYPEPTIDES
4532089
Charles Weissmann, Zurich, Switzerland assigned to Biogen N V
METHOD OF PREPARING SIZE LIPOSOMES
GIANT
Recombinant DNA molecules and hosts transformed with them which produce polypeptides displaying a biological or immunological activity of human interferon, the gene coding for these polypeptides and methods of making and
Robert C MacDonald assigned to Northwestern University Large size liposomes are produced from small unilamellar vesicles by incorporating a polar 332
333
PATENT ABSTRACTS compound in an aqueous dispersion of the vesicles, subjecting the dispersion to repeated freeze-thaw cycles, and thereafter dialyzing the vesicles against a hypoosmotic medium. The freeze-thaw cycles aggregate the vesicles and increase their internal concentration of polar compound. During dialysis the vesicles imbibe water, rupture, and form enlarged liposomes.
monocellular organisms that may be present and to eliminate larger synthetic erythrocytes. The synthetic erythrocyte preparation, if dried under vacuum to remove the major portion of the water from the encapsulated hemoglobin fraction, substantially transforms the erythrocytes but retains the integrity of the encapsulating lipid composition membranes. In the dried form, the sterile erythrocytes are storable for extended periods.
4532123 DUAL MICROCAPSULES AND PROCESS FOR THEIR PREPARATION David L Gardner assigned to Battelle Development Corporation Dual Microcapsules are disclosed. The outer membrane encapsulates a liquid having one or more smaller microcapsules (MiniMicrocapsules) suspended therein. The MiniMicrocapsules contain a conjugate or a reaction product of a Drug which diffuses into the liquid in which Mini-Microcapsules are suspended. The suspending liquid contains an enzyme which reacts with Drug complex or reaction product to regenerate or release the Drug. The drug diffuses through the outer membrane into a host.
4532130 PREPARATION OF SYNTHETIC FRYTHROCYTES Ljubomir Djordjevich, Anthony Ivankovich, William Gottschalk assigned to RushPresbyterian-St Luke's Medical Center A sterile preparation of,synthetic erythrocytes consisting of hemoglobin fraction encapsulated within water-immiscible amphiphylic membranes provides a total hemoglobin of at least about 12 gm percent at a hematocrit of 50~o. A lipid composition is prepared and dispersed by agitation in a sterile, stroma-free 30-45 gram percent hemoglobin fraction. The dispersion is pressurized to between about 400 to about 900 kg/cm2, and the pressure is substantially instantaneously released by passing the mixture to a lower pressure region through an orifice having an area of between about 0.1 and about 10.0 ram2, thereby forming the synthetic erythrocytes. The preparation is filtered through a filter which passes particles having a diameter less than about 0.22 micron to eliminate any
4532215 ISOLATION OF HEPATITIS VIRUS STRAIN HM-175
A
Richard J Daemer, Stephen Feinstone, Ian D Gust, Robert H Purcell assigned to The United States of America as represented by the Department of Health and Human Services Human hepatitis A virus (HAV), taken directly from human clinical specimens, can be isolated and serially passaged in primary African green monkey kidney (AGMK) cell cultures. This strain induced antibody to HAV in inoculated chimpanzees and is useful for vaccine.
4533497 N-ETHYLIDENE
AZETIDINONES
Douglas O Spry assigned to Eli Lilly and Company N-ethylidene azetidinones are new antibiotic compounds. They are produced from the reaction of a Grignard reagent with a cephalosporin substituted at the 3 position with a chlorn, oxy, amino or sulfide moieties. ~3~31 FERMENTATION PROCESS FOR PREPARATION OF ANTIBIOTIC BU-2517 Hiroshi Kawaguchi, Masataka Konishi, Koko Sugawara, Koji Tomita, Tokyo, Japan assigned to Bristol-Myers Company Disclosed is a process for preparation of the water-soluble peptide antibiotic designated Bu2517 by fermentation of Empedobacter sp. strain G393-B445 (ATCC 31962).
4530787
using these molecules, hosts, genes and polypeptides. The recombinant DNA molecules are characterized by structural genes that code for a polypeptide displaying a biological or immulogical activity of human interferon. In appropriate hosts these molecules permit the production and identification of genes and polypeptides displaying a biological or immunological activity of human interferon and their use in antiviral and antitumor or anticancer agents.
CONTROLLED OXIDATION OF MICROBIALLY PRODUCED CYSTEINE-CONTAINING PROTEINS Ze'e Shaked, Sidney Wolfe assigned to Cetus Corporation Method of oxidizing reduced cysteinecontaining microbially produced synthetic proteins, such as synthetic IFN- beta or synthetic IL-2, in a controlled manner so that the synthetic proteins have the same disulfide bridging as their native counterparts. The oxidation employs oiodosobenzoate as oxidizing agent and is carried out in an aqueous medium at a pH at least about one-half pH unit less than the pKa of the cysteines to be oxidized, a synthetic protein concentration of less than about 5 mg/ml, and an oxidizing agent:protein tool ratio that is at least stoichiometric, provided that the oxidizing agent is in excess in the terminal portion of the reaction.
4530908 DIAGNOSIS AND TREATMENT OF FLUKE INFECTIONS WITH MONOCLONAL ANTIBODIES Mette Strand assigned to The Johns Hopkins University Antibodies and antigens are disclosed which provide a method of detecting and a method of combating flukes. A diagnostic method for the determination of an active fluke infection in a warm-blooded animal is provided which comprises the testing of body serum or body fluids for the presence of fluke spine glycoprotein or anti-spine antibodies. Fused cell hybrids ATCC HB-8086, ATCC HB-8087 and ATCC HB-8088, as well as the antibodies produced by the fused cell hybrids, are also provided.
4530901 RECOMBINANT DNA MOLECULES AND THEIR USE IN PRODUCING HUMAN INTERFERON-LIKE POLYPEPTIDES
4532089
Charles Weissmann, Zurich, Switzerland assigned to Biogen N V
METHOD OF PREPARING SIZE LIPOSOMES
GIANT
Recombinant DNA molecules and hosts transformed with them which produce polypeptides displaying a biological or immunological activity of human interferon, the gene coding for these polypeptides and methods of making and
Robert C MacDonald assigned to Northwestern University Large size liposomes are produced from small unilamellar vesicles by incorporating a polar 332
333
PATENT ABSTRACTS compound in an aqueous dispersion of the vesicles, subjecting the dispersion to repeated freeze-thaw cycles, and thereafter dialyzing the vesicles against a hypoosmotic medium. The freeze-thaw cycles aggregate the vesicles and increase their internal concentration of polar compound. During dialysis the vesicles imbibe water, rupture, and form enlarged liposomes.
monocellular organisms that may be present and to eliminate larger synthetic erythrocytes. The synthetic erythrocyte preparation, if dried under vacuum to remove the major portion of the water from the encapsulated hemoglobin fraction, substantially transforms the erythrocytes but retains the integrity of the encapsulating lipid composition membranes. In the dried form, the sterile erythrocytes are storable for extended periods.
4532123 DUAL MICROCAPSULES AND PROCESS FOR THEIR PREPARATION David L Gardner assigned to Battelle Development Corporation Dual Microcapsules are disclosed. The outer membrane encapsulates a liquid having one or more smaller microcapsules (MiniMicrocapsules) suspended therein. The MiniMicrocapsules contain a conjugate or a reaction product of a Drug which diffuses into the liquid in which Mini-Microcapsules are suspended. The suspending liquid contains an enzyme which reacts with Drug complex or reaction product to regenerate or release the Drug. The drug diffuses through the outer membrane into a host.
4532130 PREPARATION OF SYNTHETIC FRYTHROCYTES Ljubomir Djordjevich, Anthony Ivankovich, William Gottschalk assigned to RushPresbyterian-St Luke's Medical Center A sterile preparation of,synthetic erythrocytes consisting of hemoglobin fraction encapsulated within water-immiscible amphiphylic membranes provides a total hemoglobin of at least about 12 gm percent at a hematocrit of 50~o. A lipid composition is prepared and dispersed by agitation in a sterile, stroma-free 30-45 gram percent hemoglobin fraction. The dispersion is pressurized to between about 400 to about 900 kg/cm2, and the pressure is substantially instantaneously released by passing the mixture to a lower pressure region through an orifice having an area of between about 0.1 and about 10.0 ram2, thereby forming the synthetic erythrocytes. The preparation is filtered through a filter which passes particles having a diameter less than about 0.22 micron to eliminate any
4532215 ISOLATION OF HEPATITIS VIRUS STRAIN HM-175
A
Richard J Daemer, Stephen Feinstone, Ian D Gust, Robert H Purcell assigned to The United States of America as represented by the Department of Health and Human Services Human hepatitis A virus (HAV), taken directly from human clinical specimens, can be isolated and serially passaged in primary African green monkey kidney (AGMK) cell cultures. This strain induced antibody to HAV in inoculated chimpanzees and is useful for vaccine.
4533497 N-ETHYLIDENE
AZETIDINONES
Douglas O Spry assigned to Eli Lilly and Company N-ethylidene azetidinones are new antibiotic compounds. They are produced from the reaction of a Grignard reagent with a cephalosporin substituted at the 3 position with a chlorn, oxy, amino or sulfide moieties. ~3~31 FERMENTATION PROCESS FOR PREPARATION OF ANTIBIOTIC BU-2517 Hiroshi Kawaguchi, Masataka Konishi, Koko Sugawara, Koji Tomita, Tokyo, Japan assigned to Bristol-Myers Company Disclosed is a process for preparation of the water-soluble peptide antibiotic designated Bu2517 by fermentation of Empedobacter sp. strain G393-B445 (ATCC 31962).