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4545986 Timothy grass antigen specific anti-idiotypic antibodies
PATENT ABSTRACTS
175
4~M39
4~5~5
PRODUCTION AND USE OF MONOCLONAL ANTIBODIES TO PHOSPHOTYROSINECONTAINING PROTEINS
PSEUDOMONAS EXOTOXIN CONJUGATE IMMUNOTOXINS
A Raymond Frackelton, Herman N Eisen, Alonzo H Ross assigned to Massachusetts Institute of Technology A hybridoma cell line is disclosed that secretes monoclonal antibodies which serve as a high titer, reproducible, biological reagent useful in biological/medical research for isolating and identifying phosphotyrosine-containing proteins. In addition, the antibodies have potential uses in diagnosis of a variety of diseases, including certain cancers. The antibodies, which have demonstrated affinity for a variety of molecules containing o-phosphotyrosine residues, were prepared using a synthetic analog, p-azobenzyl phosphonate (ABP) covalently linked to a carrier protein, as the antigen.
Ira Pastan. Mark C Willingham, David J Fitzgerald assigned to The United States of America as represented by the Secretary Dept of Health and Human Services A method of modifying Pseudomonas exotoxin (PE) with methyl-4-mercaptobutyrimidate is disclosed so that after conjugating the exotoxin to a monoclonal antibody (ab) such as the antibody to the transferrin receptor, the PE-ab conjugate becomes a highly potent immunotoxin suitable for use against human tumor cells. This same method has been used to conjugate PE to epidermal growth factor (EGF) to create a highly potent growth factor-toxin conjugate for use against cells having large numbers of EGF receptors. Also disclosed are the immunotoxin conjugates for Pseudomonas exotoxin coupled to anti-TFR (antibody to the transferrin receptor) and anti-TAC (antibody to the human T-cell growth factor receptor) and to EGF.
4544545 LIPOSOMES CONTAINING MODIFIED CHOLESTEROL FOR ORGAN TARGETING Patrick J Ryan, Michael A Davis, Donald L Melchior assigned to Trustees University of Massachusetts Phospholipid liposomes are provided having an outer layer including a cholesterol derivative such as a cholesterol ester and an aqueous medium confined by the layer which includes a tracer agent, a cytoxic agent or a therapeutic agent. The liposomes are adapted for specific organ targeting.
4544634 METHOD OF PRODUCING ACYCLOVIR Thomas A Krenitsky assigned to Burroughs Wellcome Co The novel compound 6-deoxyacyclovir is enzymatically converted to acyclovir by xanthine oxidase/dehydrogenase or aldehyde oxidase in vivo, i.e. within the body of the animal being treated. The enzymatic conversion may also be effected ex vivo (i.e. in vitro) as a method of syntbesizing acyclovir.
4545986 TIMOTHY GRASS ANTIGEN SPECIFIC ANTI-IDIOTYPIC ANTIBODIES Arthur Malley assigned to Research Corporation This invention provides a number of immunological reagents useful for the suppression of allergic reactions induced by Timothy-grass pollen allergen (or cross-reacting allergens thereof) and for methods of obtaining said reagents. The reagents include: (1) an anti-idiotypic antibody (anti-lgEid) directed to the allergen (Timothy grass antigen) binding site of an igE immunoglobulin; (2) an anti-idiotypic antibody directed to the allergenic T-cell helper factor (THF) derived from Timothy grass pollen antigen stimulated lymphocytes; (3) unique methods for generating antigen-specific T-suppressor cells (TS): (4) a unique method for producing antigenspecific suppressor T cell factors (TSF); (5) a method for fractionation of TSF; and (6) a specific T cell factor, TSF2. The invention further provides pharmaceutical compositions comprising the above anti-idiotypic antibodies or suppressor factor derived from Ts cells.
175
4~M39
4~5~5
PRODUCTION AND USE OF MONOCLONAL ANTIBODIES TO PHOSPHOTYROSINECONTAINING PROTEINS
PSEUDOMONAS EXOTOXIN CONJUGATE IMMUNOTOXINS
A Raymond Frackelton, Herman N Eisen, Alonzo H Ross assigned to Massachusetts Institute of Technology A hybridoma cell line is disclosed that secretes monoclonal antibodies which serve as a high titer, reproducible, biological reagent useful in biological/medical research for isolating and identifying phosphotyrosine-containing proteins. In addition, the antibodies have potential uses in diagnosis of a variety of diseases, including certain cancers. The antibodies, which have demonstrated affinity for a variety of molecules containing o-phosphotyrosine residues, were prepared using a synthetic analog, p-azobenzyl phosphonate (ABP) covalently linked to a carrier protein, as the antigen.
Ira Pastan. Mark C Willingham, David J Fitzgerald assigned to The United States of America as represented by the Secretary Dept of Health and Human Services A method of modifying Pseudomonas exotoxin (PE) with methyl-4-mercaptobutyrimidate is disclosed so that after conjugating the exotoxin to a monoclonal antibody (ab) such as the antibody to the transferrin receptor, the PE-ab conjugate becomes a highly potent immunotoxin suitable for use against human tumor cells. This same method has been used to conjugate PE to epidermal growth factor (EGF) to create a highly potent growth factor-toxin conjugate for use against cells having large numbers of EGF receptors. Also disclosed are the immunotoxin conjugates for Pseudomonas exotoxin coupled to anti-TFR (antibody to the transferrin receptor) and anti-TAC (antibody to the human T-cell growth factor receptor) and to EGF.
4544545 LIPOSOMES CONTAINING MODIFIED CHOLESTEROL FOR ORGAN TARGETING Patrick J Ryan, Michael A Davis, Donald L Melchior assigned to Trustees University of Massachusetts Phospholipid liposomes are provided having an outer layer including a cholesterol derivative such as a cholesterol ester and an aqueous medium confined by the layer which includes a tracer agent, a cytoxic agent or a therapeutic agent. The liposomes are adapted for specific organ targeting.
4544634 METHOD OF PRODUCING ACYCLOVIR Thomas A Krenitsky assigned to Burroughs Wellcome Co The novel compound 6-deoxyacyclovir is enzymatically converted to acyclovir by xanthine oxidase/dehydrogenase or aldehyde oxidase in vivo, i.e. within the body of the animal being treated. The enzymatic conversion may also be effected ex vivo (i.e. in vitro) as a method of syntbesizing acyclovir.
4545986 TIMOTHY GRASS ANTIGEN SPECIFIC ANTI-IDIOTYPIC ANTIBODIES Arthur Malley assigned to Research Corporation This invention provides a number of immunological reagents useful for the suppression of allergic reactions induced by Timothy-grass pollen allergen (or cross-reacting allergens thereof) and for methods of obtaining said reagents. The reagents include: (1) an anti-idiotypic antibody (anti-lgEid) directed to the allergen (Timothy grass antigen) binding site of an igE immunoglobulin; (2) an anti-idiotypic antibody directed to the allergenic T-cell helper factor (THF) derived from Timothy grass pollen antigen stimulated lymphocytes; (3) unique methods for generating antigen-specific T-suppressor cells (TS): (4) a unique method for producing antigenspecific suppressor T cell factors (TSF); (5) a method for fractionation of TSF; and (6) a specific T cell factor, TSF2. The invention further provides pharmaceutical compositions comprising the above anti-idiotypic antibodies or suppressor factor derived from Ts cells.