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4554249 Method for the quantitative determination of physiological components in biological fluids
PATENT ABSTRACTS 4550163
309 4551275
LIGAND ANALOGqRREVERSIBLE ENZYME INHIBITOR CONJUGATES
DESMETHYLIMIPRAMINE DERIVATIVES AND POLY(AMINO ACID) CONJUGATES
Houston F Voss, Jacob Plattner, Thomas Herrin assigned to Abbott Laboratories
Marcel R Pirio, Prithipal Singh assigned to Syntex (U S A ) Inc
The present invention encompasses a method for determining ligands in test samples comprising intermixing with the test sample a ligand analog-irreversible enzyme inhibitor conjugate and a binding protein bindable to the ligand and the ligand analog-irreversible enzyme inhibitor conjugate and wherein the amount of ligand analog-irreversible enzyme inhibitor conjugate bound by the binding protein is related to the amount of ligand in the test sample, said binding protein inactivating the irreversible enzyme inhibitor when bound to the iigand analog portion of the conjugate; intermixing an enzyme which is irreversibly inhibited by the ligand analogirreversible enzyme inhibitor conjugate unbound by the binding protein; and intermixing substrate to the enzyme and monitoring the enzyme substrate reaction. The invention also includes ligand analog-irreversible enzyme inhibitor conjugates useful as reagents in practicing the method. Methods and reagents of the present are particularly useful in determining drugs, hormones, and the like in biological fluids.
Desmethylimipramine functionalized compounds are provided for conjugation to antigenic compounds, particularly poly(amino acids) and enzymes. The antigenic conjugates are employed for the production of antibodies, which find particular use in immunoassays for the determination of desmethyIimipramine, while the enzyme conjugate finds use in an enzyme assay for the determination of desmethylimipramine.
4554249 METHOD FOR THE QUANTITATIVE DETERMINATION OF PHYSIOLOGICAL COMPONENTS IN BIOLOGICAL FLUIDS Akira Kosaka, Sawao Murao, Kenich Hirano, Noriak Tanaka, Kuniyosh Matsunaga, Seto, Japan assigned to Amano Pharmaceutical Company Limited
4551243 ANAEROBIC DIGESTER John H Martin assigned to Energy Cycle Inc To reduce the accumulation of undesirable solid material within an anaerobic digester, new slurry for digesting is injected into the digester at locations extending across the horizontal width at one end, parallel to a zone of floating solids in a concentration of solid material between ten and twelve percent, with a momentum of at least 0.1 pound-foot per second. Liquid is taken out of the digester across a trough extending across the width of the digester. Bubbles are broken above the liquid level of the digester by a sieve or surfactant or combination of the two.
A novel enzyme of bilirubin oxidase produced by a genus Myrothecium or genus Coprinus origin microorganism and a conventional enzyme of laccase are found, in the presence of a specific additive compound, e.g. a surface active agent, aromatic carboxylic acid, sulfa drug or protease, to oxidize both conjugated and unconjugated bilirubin in biological fluid to biliverdin without formation of hydrogen peroxide, such that in the case of conventional enzymatic methods of the quantitative determination of glucose, cholesterol, neutral fats, free fatty acids, phospholipids or uric acid all existing together with bilirubin in biological fluid, the usual interference with such determination, as otherwise caused.by bilirubin coexisting in such fluid, can be prevented by adding such a bilirubin oxidase or iaccase together with such a specific additive compound to the determinative reaction system.
309 4551275
LIGAND ANALOGqRREVERSIBLE ENZYME INHIBITOR CONJUGATES
DESMETHYLIMIPRAMINE DERIVATIVES AND POLY(AMINO ACID) CONJUGATES
Houston F Voss, Jacob Plattner, Thomas Herrin assigned to Abbott Laboratories
Marcel R Pirio, Prithipal Singh assigned to Syntex (U S A ) Inc
The present invention encompasses a method for determining ligands in test samples comprising intermixing with the test sample a ligand analog-irreversible enzyme inhibitor conjugate and a binding protein bindable to the ligand and the ligand analog-irreversible enzyme inhibitor conjugate and wherein the amount of ligand analog-irreversible enzyme inhibitor conjugate bound by the binding protein is related to the amount of ligand in the test sample, said binding protein inactivating the irreversible enzyme inhibitor when bound to the iigand analog portion of the conjugate; intermixing an enzyme which is irreversibly inhibited by the ligand analogirreversible enzyme inhibitor conjugate unbound by the binding protein; and intermixing substrate to the enzyme and monitoring the enzyme substrate reaction. The invention also includes ligand analog-irreversible enzyme inhibitor conjugates useful as reagents in practicing the method. Methods and reagents of the present are particularly useful in determining drugs, hormones, and the like in biological fluids.
Desmethylimipramine functionalized compounds are provided for conjugation to antigenic compounds, particularly poly(amino acids) and enzymes. The antigenic conjugates are employed for the production of antibodies, which find particular use in immunoassays for the determination of desmethyIimipramine, while the enzyme conjugate finds use in an enzyme assay for the determination of desmethylimipramine.
4554249 METHOD FOR THE QUANTITATIVE DETERMINATION OF PHYSIOLOGICAL COMPONENTS IN BIOLOGICAL FLUIDS Akira Kosaka, Sawao Murao, Kenich Hirano, Noriak Tanaka, Kuniyosh Matsunaga, Seto, Japan assigned to Amano Pharmaceutical Company Limited
4551243 ANAEROBIC DIGESTER John H Martin assigned to Energy Cycle Inc To reduce the accumulation of undesirable solid material within an anaerobic digester, new slurry for digesting is injected into the digester at locations extending across the horizontal width at one end, parallel to a zone of floating solids in a concentration of solid material between ten and twelve percent, with a momentum of at least 0.1 pound-foot per second. Liquid is taken out of the digester across a trough extending across the width of the digester. Bubbles are broken above the liquid level of the digester by a sieve or surfactant or combination of the two.
A novel enzyme of bilirubin oxidase produced by a genus Myrothecium or genus Coprinus origin microorganism and a conventional enzyme of laccase are found, in the presence of a specific additive compound, e.g. a surface active agent, aromatic carboxylic acid, sulfa drug or protease, to oxidize both conjugated and unconjugated bilirubin in biological fluid to biliverdin without formation of hydrogen peroxide, such that in the case of conventional enzymatic methods of the quantitative determination of glucose, cholesterol, neutral fats, free fatty acids, phospholipids or uric acid all existing together with bilirubin in biological fluid, the usual interference with such determination, as otherwise caused.by bilirubin coexisting in such fluid, can be prevented by adding such a bilirubin oxidase or iaccase together with such a specific additive compound to the determinative reaction system.