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4560648 Homogeneous enzyme immunoassay for ferritin
PATENT ABSTRACTS
312 4559310
ASSAY METHODS AND SYSTEMS UTILIZING MAST CELL CLONES Harvey I Cantor, Gary Nabel assigned to Dana Farber Cancer Institute The present invention is directed to an in vitro assay, useful in determining the effectiveness of anti-allergy compounds and/or useful in measuring the degree of sensitivity of a patient to particular allergens. The present invention permits potential anti-allergy agents to be assayed in a number of ways. For example, the binding and dissociation rates of IgE to the mast cells in the presence and the absence of the substance being tested may be measured thereby giving a direct indication of that substance's ability to interfere with the IgE binding reaction. Another measure of a substance's potential as an anti-allergy agent is based upon the release of mediators or other compounds from the mast cells after sensitization by the allergen and exposure of the sensitized cells to the allergen. The present invention generally involves the following steps: (a) sensitizing cloned mast cells to an allergen; (b) exposing sensitized mast cells to the allergen in the presence of a test anti-allergy agent; and (c) measuring the reaction products of step (b) for an indication of test compound effect. 4560504 CARBOXYL ANCHORED IMMOBILIZED ANTIBODIES Edward C Arnold assigned to UOP lnc An immobilized antibody system can be made by reacting an aminated core support with an antibody in the presence of a condensing agent which promotes the formation of the amide linkage. The immobilized antibody system is highly resistant to leaching, may be made incompressible, sterilizable, and pyrogen-free. Such an immobilized antibody system is well suited for repeated use with minimal change in its physical and biochemical properties.
456O648 HOMOGENEOUS ENZYME IMMUNOASSAY FOR FERRITIN Richar Armenta assigned to Syntex (U S A ) Inc
Composition and method are provided for enzyme immunoassays for high molecular weight proteins, such as ferritin. An enzyme is conjugated to the high molecular weight protein through a specific linking group. Usually, on the average, about 2 to 10 enzyme molecules are bonded to each protein molecule. In an assay, the conjugate competes with an unknown sample for receptor and the resulting enzymatic activity is compared to a standard for a determination of the presence and amount of protein in the unknown.
4560649 ASSAYING FOR HLH OR HCG WITH IMMOBILIZED HORMONE RECEPTORS Brij B Saxena, Premil Rathnam assigned to Cornell Research Foundation A method to obtain a substantially pure and stable hLH-hCG receptor from gonadal cells is disclosed. The characteristics of the purified receptor are disclosed. The receptor, in addition to being used as receptor in an enzyme- or dyereceptor assay for hLH and/or hCG, can be used to purify hLH or hCG used in the preparation of standard dye-hormone or enzyme-hormone complexes. These complexes can be used in enzyme- or dye-immunoassays, as well as the receptor assay.
4560650 METHOD AND COMPOSITIONS FOR DETERMINATION OF GAMMA GLUTAMYL TRANSPEPTIDASE Henry W Bauer, Ravindra S Shukla assigned to Coulter Electronics Inc A two-reagent assay system for determination of gamma glutamyl transpeptidase enzyme activity in a biological fluid. Gamma glutamyl-pnitroanalide substrate for the enzymatic reaction is provided in a first reagent which is a dry mixture of the substrate with a normally solid acid which is water soluble and a second reagent includes a buffer and a component selected for reception of the glutamyl product of the enzymatic reaction.
312 4559310
ASSAY METHODS AND SYSTEMS UTILIZING MAST CELL CLONES Harvey I Cantor, Gary Nabel assigned to Dana Farber Cancer Institute The present invention is directed to an in vitro assay, useful in determining the effectiveness of anti-allergy compounds and/or useful in measuring the degree of sensitivity of a patient to particular allergens. The present invention permits potential anti-allergy agents to be assayed in a number of ways. For example, the binding and dissociation rates of IgE to the mast cells in the presence and the absence of the substance being tested may be measured thereby giving a direct indication of that substance's ability to interfere with the IgE binding reaction. Another measure of a substance's potential as an anti-allergy agent is based upon the release of mediators or other compounds from the mast cells after sensitization by the allergen and exposure of the sensitized cells to the allergen. The present invention generally involves the following steps: (a) sensitizing cloned mast cells to an allergen; (b) exposing sensitized mast cells to the allergen in the presence of a test anti-allergy agent; and (c) measuring the reaction products of step (b) for an indication of test compound effect. 4560504 CARBOXYL ANCHORED IMMOBILIZED ANTIBODIES Edward C Arnold assigned to UOP lnc An immobilized antibody system can be made by reacting an aminated core support with an antibody in the presence of a condensing agent which promotes the formation of the amide linkage. The immobilized antibody system is highly resistant to leaching, may be made incompressible, sterilizable, and pyrogen-free. Such an immobilized antibody system is well suited for repeated use with minimal change in its physical and biochemical properties.
456O648 HOMOGENEOUS ENZYME IMMUNOASSAY FOR FERRITIN Richar Armenta assigned to Syntex (U S A ) Inc
Composition and method are provided for enzyme immunoassays for high molecular weight proteins, such as ferritin. An enzyme is conjugated to the high molecular weight protein through a specific linking group. Usually, on the average, about 2 to 10 enzyme molecules are bonded to each protein molecule. In an assay, the conjugate competes with an unknown sample for receptor and the resulting enzymatic activity is compared to a standard for a determination of the presence and amount of protein in the unknown.
4560649 ASSAYING FOR HLH OR HCG WITH IMMOBILIZED HORMONE RECEPTORS Brij B Saxena, Premil Rathnam assigned to Cornell Research Foundation A method to obtain a substantially pure and stable hLH-hCG receptor from gonadal cells is disclosed. The characteristics of the purified receptor are disclosed. The receptor, in addition to being used as receptor in an enzyme- or dyereceptor assay for hLH and/or hCG, can be used to purify hLH or hCG used in the preparation of standard dye-hormone or enzyme-hormone complexes. These complexes can be used in enzyme- or dye-immunoassays, as well as the receptor assay.
4560650 METHOD AND COMPOSITIONS FOR DETERMINATION OF GAMMA GLUTAMYL TRANSPEPTIDASE Henry W Bauer, Ravindra S Shukla assigned to Coulter Electronics Inc A two-reagent assay system for determination of gamma glutamyl transpeptidase enzyme activity in a biological fluid. Gamma glutamyl-pnitroanalide substrate for the enzymatic reaction is provided in a first reagent which is a dry mixture of the substrate with a normally solid acid which is water soluble and a second reagent includes a buffer and a component selected for reception of the glutamyl product of the enzymatic reaction.