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4562250 Steroidal glycosides produced by Yucca tissue culture
PATENT ABSTRACTS
344
A new antibacterially active agent has been isolated from Streptomyces clavuligerus. This new compound which is designated clavulanic acid has the formula (I): See Patent for Chemical Structure (I) In addition to being a broad spectrum antibiotic of medium potency, clavulanic acid and its salts and esters have the ability to enhance the effectiveness of beta lactam antibiotics against many beta-lactamase producing bacteria. 4560662 STREPTOMYCES ANTIBIOTICUS A T C C 31771 A N D P R O C E S S F O R OBTAINING SAME
in vitro is a source of gp90 antigen for generation of monoclonal antibodies which will be useful in analyzing the gp90 antigen for those epitopes which may be of diagnostic value in immunoassay of melanoma.
45622~ STEROIDAL GLYCOSIDES PRODUCED BY YUCCA TISSUE CULTURE E John Staba, Jean J MacCarthy assigned to Regents of the University of Minnesota
45621~
Methods for the production of steroids (sapogenins) and steroidal glycosides (saponins) from cell, root-cell and shoot tissue cultures of the Yucca plant. The cultures were established and maintained in a suitable nutrient plant cell medium supplemented with growth regulators. The desired products are extracted from the cultures using conventional extraction techniques. Greater total production of steroidal glycosides occurred in root-cell, cell, and shoot tissue cultures in that order. Although the cultures may be grown under conditions ranging from total darkness to total light, steroidal glycoside production was highest in root-cell cultures grown continuously in the light as compared to those grown in the dark. Root-cell culture senescence also enhanced the yield of total steroidal glycosides. Steroids are obtained from the steroidal glycosides by acid hydrolysis to remove the sugar moieties. The production of radiolabeled steroids and steroidal glycosides is disclosed.
MELANOMA TUMOR ANTIGEN AND AUTOLOGOUS ANTIBODY
4563303
Leonardo M Cappelletti, Roberto Spagnoli, Luciano Toscano assigned to Pierrel S p A The fermentation of a substrate selected among erythronolide B, erythronolide A and erythronolide A oxime with a novel mutant, Streptomyces antibioticus ATCC 31771, obtained from an industrial stock for the production of oleandomycin, said novel mutant being incapable of producing the same oleandomycin, permits novel macrolide antibiotics to be produced, having not only an activity range like that of erythromycin, but characterized by a greater stability in acidic environment, whereby for the administration of the antibiotic it is no longer necessary to have recourse to esters and/or salts highly toxic for the organism.
FranCisco X Real, M Jule Mattes, Alan N Houghton, Philip O Livingston, Kenneth Lloyd, Herbert Oettgen, Lloyd J Old assigned to SloanKettering Institute The present invention concerns novel immunoprecipitating autologous antibodies which recognize the Class I gp90 antigen on melanoma cells. These antibodies, optionally tagged with a chromophoric or radioactive label and immobilized on an inert support, may be used to recognize and isolate the gp90 antigen from melanoma cell extracts. Monoclonal antibodies to melanoma may be screened with the gp90 antigen for those which recognize epitopes other than the FD antigenic system. The cell line containing the gp90 antigen which has been cultured
METHOD FOR PURIFICATION OF FILAMENTOUS HEMAGGLUTININ Akihiro Ginnaga, Shin Sakuma, Tsukasa Nishihara, Tomitaka Tashiro, Sadao Susumi, Tetsuo Kawahara, Hiroshi Mizokami, Kumamoto, Japan assigned to Judicial Foundation The Chemosero-Therapeutic Research Institute Improved method for the purification of filamentous hemagglutinin (F-HA) on industrial scale which comprises contacting a culture of a microorganism of the genus Bordetella with a cellulose sulfate gel, a polysaccharide gel chemically bound with dextran sulfate, or a cross-
344
A new antibacterially active agent has been isolated from Streptomyces clavuligerus. This new compound which is designated clavulanic acid has the formula (I): See Patent for Chemical Structure (I) In addition to being a broad spectrum antibiotic of medium potency, clavulanic acid and its salts and esters have the ability to enhance the effectiveness of beta lactam antibiotics against many beta-lactamase producing bacteria. 4560662 STREPTOMYCES ANTIBIOTICUS A T C C 31771 A N D P R O C E S S F O R OBTAINING SAME
in vitro is a source of gp90 antigen for generation of monoclonal antibodies which will be useful in analyzing the gp90 antigen for those epitopes which may be of diagnostic value in immunoassay of melanoma.
45622~ STEROIDAL GLYCOSIDES PRODUCED BY YUCCA TISSUE CULTURE E John Staba, Jean J MacCarthy assigned to Regents of the University of Minnesota
45621~
Methods for the production of steroids (sapogenins) and steroidal glycosides (saponins) from cell, root-cell and shoot tissue cultures of the Yucca plant. The cultures were established and maintained in a suitable nutrient plant cell medium supplemented with growth regulators. The desired products are extracted from the cultures using conventional extraction techniques. Greater total production of steroidal glycosides occurred in root-cell, cell, and shoot tissue cultures in that order. Although the cultures may be grown under conditions ranging from total darkness to total light, steroidal glycoside production was highest in root-cell cultures grown continuously in the light as compared to those grown in the dark. Root-cell culture senescence also enhanced the yield of total steroidal glycosides. Steroids are obtained from the steroidal glycosides by acid hydrolysis to remove the sugar moieties. The production of radiolabeled steroids and steroidal glycosides is disclosed.
MELANOMA TUMOR ANTIGEN AND AUTOLOGOUS ANTIBODY
4563303
Leonardo M Cappelletti, Roberto Spagnoli, Luciano Toscano assigned to Pierrel S p A The fermentation of a substrate selected among erythronolide B, erythronolide A and erythronolide A oxime with a novel mutant, Streptomyces antibioticus ATCC 31771, obtained from an industrial stock for the production of oleandomycin, said novel mutant being incapable of producing the same oleandomycin, permits novel macrolide antibiotics to be produced, having not only an activity range like that of erythromycin, but characterized by a greater stability in acidic environment, whereby for the administration of the antibiotic it is no longer necessary to have recourse to esters and/or salts highly toxic for the organism.
FranCisco X Real, M Jule Mattes, Alan N Houghton, Philip O Livingston, Kenneth Lloyd, Herbert Oettgen, Lloyd J Old assigned to SloanKettering Institute The present invention concerns novel immunoprecipitating autologous antibodies which recognize the Class I gp90 antigen on melanoma cells. These antibodies, optionally tagged with a chromophoric or radioactive label and immobilized on an inert support, may be used to recognize and isolate the gp90 antigen from melanoma cell extracts. Monoclonal antibodies to melanoma may be screened with the gp90 antigen for those which recognize epitopes other than the FD antigenic system. The cell line containing the gp90 antigen which has been cultured
METHOD FOR PURIFICATION OF FILAMENTOUS HEMAGGLUTININ Akihiro Ginnaga, Shin Sakuma, Tsukasa Nishihara, Tomitaka Tashiro, Sadao Susumi, Tetsuo Kawahara, Hiroshi Mizokami, Kumamoto, Japan assigned to Judicial Foundation The Chemosero-Therapeutic Research Institute Improved method for the purification of filamentous hemagglutinin (F-HA) on industrial scale which comprises contacting a culture of a microorganism of the genus Bordetella with a cellulose sulfate gel, a polysaccharide gel chemically bound with dextran sulfate, or a cross-