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4652517 Methods for the in vitro detection and identification of unknown pathogens or genetic entities
PATENT ABSTRACTS
390
4652450 BACTERIAL FSH BINDING INHIBITOR Patrick M Sluss. Leo E Reichert Research Corporation
assigned
to
This invention relates to an FSH-binding inhibitor produced by microorganisms of the genus Serratia.
4652517 METHODS FOR THE IN VITRO DETECTION AND IDENTIFICATION OF UNKNOWN PATHOGENS OR GENETIC ENTITIES David R Scholl, Joseph Jollick assigned to Diagnostic Research Limited Partnership Methods for the detection and identification of unknown pathogens or genetic entities from samples suspected of containing the same involve the use of novel wicking techniques whereby sample DNA/RNA becomes readily available on a porous, inert, positively charged support in single stranded form for hybridization with a hybridization probe having a nucleotide sequence substantially complementary to a nucleotide sequence of the sample DNA/RNA. Such methods permit the detection and identification of unknown pathogens and genetic entities in a shorter period of time than that required by conventional methods. Lysis solutions for rapidly lysing bacterial cells without the aid of enzymes are also disclosed.
465252 1 METHOD FOR THE EARLY DIAGNOSIS OF BORDETELLA DISEASES AND KIT THEREFOR Dennis L Confer, John W Eaton assigned to Regents of the University of Minnesota A method for the early diagnosis of Bordetella diseases based on the detection of Bordetella adenylate cyclase and the ability of this enzyme to catalyze the formation of cyclic adenosine monophosphate (CAMP). A sample secretion is taken from a suspected victim of a Bordetella disease (the most common of which in humans is
whooping cough) and Incubated under conditions to promote the formatton of CAMP. which may then be measured as evidence of the presence of the disease. A sample of nasopharyngeal secretions IS maintained m a nutrtent medium pending assay. An adenylate cydase assay is made by incubating the sample with adenosme triphosphate (ATP) and the calcium binding protein calmodulin to generate CAMP. The CAMP is measured by any of several known available methods. To facilitate diagnosis. a kit is provided containing the materials necessary for collection of secretion specimens. for storage of specimens pending assay. and transport of specimens to the assay laboratory. and for performing the assay and determining the result.
4652522 CONTINUOUS LYMPHOCYTE CELL LINES, THEIR PRODUCTION AND USE Roger H Kennett. Zdenka L Jonak assigned to The University of Pennsylvania A method for producmg continuous B lymphocyte cell lines and monoclonal antibodies by such lines is provided. DNA isolated from neoplastic cells is introduced into stimulated lymphocytes. Individual cells that have been transformed by the added DNA and that produce antibodies are clonally expanded. Cultures of these continuous cells are employed to produce monoclonal antibodies.
4654313 METHODS FOR THE DETERMINATION OF BRAIN ANTIGENS Boyd Hartman University
assigned
to The Washington
A radioimmunoassay method for the determination of brain antigens or protein such as S-100 responsible for neurological disorders is disclosed. The method involves preparing a standard blood plasma or serum formulation containing substantially none of the protein being assayed and approximately the average degree of non-specific competitive binding found in the unknown blood sample and mixing known quantities of the protein being assayed with the standard blood formulation to provide a reference standard. The unknown blood sam-
390
4652450 BACTERIAL FSH BINDING INHIBITOR Patrick M Sluss. Leo E Reichert Research Corporation
assigned
to
This invention relates to an FSH-binding inhibitor produced by microorganisms of the genus Serratia.
4652517 METHODS FOR THE IN VITRO DETECTION AND IDENTIFICATION OF UNKNOWN PATHOGENS OR GENETIC ENTITIES David R Scholl, Joseph Jollick assigned to Diagnostic Research Limited Partnership Methods for the detection and identification of unknown pathogens or genetic entities from samples suspected of containing the same involve the use of novel wicking techniques whereby sample DNA/RNA becomes readily available on a porous, inert, positively charged support in single stranded form for hybridization with a hybridization probe having a nucleotide sequence substantially complementary to a nucleotide sequence of the sample DNA/RNA. Such methods permit the detection and identification of unknown pathogens and genetic entities in a shorter period of time than that required by conventional methods. Lysis solutions for rapidly lysing bacterial cells without the aid of enzymes are also disclosed.
465252 1 METHOD FOR THE EARLY DIAGNOSIS OF BORDETELLA DISEASES AND KIT THEREFOR Dennis L Confer, John W Eaton assigned to Regents of the University of Minnesota A method for the early diagnosis of Bordetella diseases based on the detection of Bordetella adenylate cyclase and the ability of this enzyme to catalyze the formation of cyclic adenosine monophosphate (CAMP). A sample secretion is taken from a suspected victim of a Bordetella disease (the most common of which in humans is
whooping cough) and Incubated under conditions to promote the formatton of CAMP. which may then be measured as evidence of the presence of the disease. A sample of nasopharyngeal secretions IS maintained m a nutrtent medium pending assay. An adenylate cydase assay is made by incubating the sample with adenosme triphosphate (ATP) and the calcium binding protein calmodulin to generate CAMP. The CAMP is measured by any of several known available methods. To facilitate diagnosis. a kit is provided containing the materials necessary for collection of secretion specimens. for storage of specimens pending assay. and transport of specimens to the assay laboratory. and for performing the assay and determining the result.
4652522 CONTINUOUS LYMPHOCYTE CELL LINES, THEIR PRODUCTION AND USE Roger H Kennett. Zdenka L Jonak assigned to The University of Pennsylvania A method for producmg continuous B lymphocyte cell lines and monoclonal antibodies by such lines is provided. DNA isolated from neoplastic cells is introduced into stimulated lymphocytes. Individual cells that have been transformed by the added DNA and that produce antibodies are clonally expanded. Cultures of these continuous cells are employed to produce monoclonal antibodies.
4654313 METHODS FOR THE DETERMINATION OF BRAIN ANTIGENS Boyd Hartman University
assigned
to The Washington
A radioimmunoassay method for the determination of brain antigens or protein such as S-100 responsible for neurological disorders is disclosed. The method involves preparing a standard blood plasma or serum formulation containing substantially none of the protein being assayed and approximately the average degree of non-specific competitive binding found in the unknown blood sample and mixing known quantities of the protein being assayed with the standard blood formulation to provide a reference standard. The unknown blood sam-