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4654303 Construction of novel mutant microorganisms
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PATENT ABSTRACTS human T cells and cutaneous T lymphoma cells. The hybrid is formed by fusing splenocytes from immunized CAF1 mice with P3X63Ag8UI myeloma cells. Diagnostic and therapeutic uses of the monoclonal antibody are also disclosed.
4654303 CONSTRUCTION OF NOVEL MUTANT MICROORGANISMS Scott Hagedorn assigned to Celanese Corporation
4654298 METHOD FOR DETECTION OF PERITONEAL INFLAMMATION OR INFECTION James L Babb, Timothy G Bloomster, Jon A Rudbach assigned to Abbott Laboratories Peritoneal inflammation or infection can be detected in a patient by assaying a peritoneal lavage sample from the patient for lysosomal enzymes. This is done by combining the lavage sample with a leukocyte lysing agent and a chromogenic or fluorogenic enzyme substrate specific for lysosomal enzymes. The lysosomal enzymes can then be measured, and the inflammation or infection detected, by measuring the color or fluorescence developed in the sample by action of the enzymes on the substrate.
This invention provides novel mutant strains of microorganisms (e.g., Pseudomonas putida Biotype A) which are capable of converting substrates such as toluene, p-xylene, catechol and 4methylcatechol to 2-hydroxymuconic semialdehyde or substituted analog of 2hydroxymuconic semialdehyde quantitatively by the meta (catechol 2,3-oxygenase) pathway. No active 2-hydroxymuconic semialdehydemetabolizing enzymes are induced in the microorganism, thereby permitting a 2hydroxymuconic semialdehyde type metabolite to be produced and accumulated in a bioconversion medium containing the microorganism. 4657383 OPTICAL STORED
ASSAY METHOD FOR HUMAN PLATELETS
Brian J Bellhouse, lslip, Oxfordshire, United Kingdom
4654301 PROCESS FOR DETECTING LDHK ISOZYME ACTIVITY IN HUMAN SERUM FOR USE AS A DIAGNOSTIC AID AND FOR MONITORING RESPONSE TO CANCER THERAPY Garth R Anderson, Kenneth F Manly, Arnold Mittelman assigned to Health Research lnc (Roswell Park Division) An improved assay procedure for quantitatively detecting LDHk isozyme in human serum is provided which utilizes previously known electrophoretic gel separation procedures combined with an improved staining method wherein the staining solution has a constant temperature of about 37£20 + 0 C, and a pH of about 8.0, and is contacted with the gel matrix for at least 3 hours in the absence of oxygen and light. This improved assay procedure may be used as an adjunct to other procedures for diagnosing the presence of primary cancer, as a post-operative indicator of metastatic cancer and in monitoring the success of cancer therapy. + RE + RE. + RE
The viability of a pack of stored blood platelets is monitored by gripping the pack between two plates (6) and (7) which are closed to pinch together the walls of the bag (10) along an Lshaped seal (14), leaving a channel (17), squeezing a part 05) of the bag by means of a reciprocating plunger (19), so that the platelets continually flow to and fro between the part (l 5) and the part (16), through the channel (17), and passing a beam of light from an LED (27) through the channel (17). to a photoresistor (29). The AC signal from the photoresistor corresponds to the fluctuations in the intensity of the light passing through the channel 07) and the amplitude of this signal is representative of the viability, and hence the clinical acceptability, of the platelets. 4657760 METHODS AND COMPOSITIONS USING MONOCLONAL ANTIBODY TO HUMAN T CELLS Patrick C Kung, Gideo Goldstein assigned to Ortho Pharmaceutical Corporation
PATENT ABSTRACTS human T cells and cutaneous T lymphoma cells. The hybrid is formed by fusing splenocytes from immunized CAF1 mice with P3X63Ag8UI myeloma cells. Diagnostic and therapeutic uses of the monoclonal antibody are also disclosed.
4654303 CONSTRUCTION OF NOVEL MUTANT MICROORGANISMS Scott Hagedorn assigned to Celanese Corporation
4654298 METHOD FOR DETECTION OF PERITONEAL INFLAMMATION OR INFECTION James L Babb, Timothy G Bloomster, Jon A Rudbach assigned to Abbott Laboratories Peritoneal inflammation or infection can be detected in a patient by assaying a peritoneal lavage sample from the patient for lysosomal enzymes. This is done by combining the lavage sample with a leukocyte lysing agent and a chromogenic or fluorogenic enzyme substrate specific for lysosomal enzymes. The lysosomal enzymes can then be measured, and the inflammation or infection detected, by measuring the color or fluorescence developed in the sample by action of the enzymes on the substrate.
This invention provides novel mutant strains of microorganisms (e.g., Pseudomonas putida Biotype A) which are capable of converting substrates such as toluene, p-xylene, catechol and 4methylcatechol to 2-hydroxymuconic semialdehyde or substituted analog of 2hydroxymuconic semialdehyde quantitatively by the meta (catechol 2,3-oxygenase) pathway. No active 2-hydroxymuconic semialdehydemetabolizing enzymes are induced in the microorganism, thereby permitting a 2hydroxymuconic semialdehyde type metabolite to be produced and accumulated in a bioconversion medium containing the microorganism. 4657383 OPTICAL STORED
ASSAY METHOD FOR HUMAN PLATELETS
Brian J Bellhouse, lslip, Oxfordshire, United Kingdom
4654301 PROCESS FOR DETECTING LDHK ISOZYME ACTIVITY IN HUMAN SERUM FOR USE AS A DIAGNOSTIC AID AND FOR MONITORING RESPONSE TO CANCER THERAPY Garth R Anderson, Kenneth F Manly, Arnold Mittelman assigned to Health Research lnc (Roswell Park Division) An improved assay procedure for quantitatively detecting LDHk isozyme in human serum is provided which utilizes previously known electrophoretic gel separation procedures combined with an improved staining method wherein the staining solution has a constant temperature of about 37£20 + 0 C, and a pH of about 8.0, and is contacted with the gel matrix for at least 3 hours in the absence of oxygen and light. This improved assay procedure may be used as an adjunct to other procedures for diagnosing the presence of primary cancer, as a post-operative indicator of metastatic cancer and in monitoring the success of cancer therapy. + RE + RE. + RE
The viability of a pack of stored blood platelets is monitored by gripping the pack between two plates (6) and (7) which are closed to pinch together the walls of the bag (10) along an Lshaped seal (14), leaving a channel (17), squeezing a part 05) of the bag by means of a reciprocating plunger (19), so that the platelets continually flow to and fro between the part (l 5) and the part (16), through the channel (17), and passing a beam of light from an LED (27) through the channel (17). to a photoresistor (29). The AC signal from the photoresistor corresponds to the fluctuations in the intensity of the light passing through the channel 07) and the amplitude of this signal is representative of the viability, and hence the clinical acceptability, of the platelets. 4657760 METHODS AND COMPOSITIONS USING MONOCLONAL ANTIBODY TO HUMAN T CELLS Patrick C Kung, Gideo Goldstein assigned to Ortho Pharmaceutical Corporation