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4656131 Method for producing interferons
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PATENT ABSTRACTS pie is then mixed with a radioactively labeled sample of the protein being assayed and an antibody capable of immunoreactivity with the protein, the mixture is incubated, the antibody bound protein is separated from the unbound protein and the relative amounts of antibody bound and unbound protein are determined. Then, the antibody mixing, incubation and separation steps are separately repeated with an unknown blood sample from which the protein being assayed has been substantially removed to provide information for the calculation of a corrected protein level. The amount of the protein being assayed is then determined from the reference standard and the separate determinations made with the unknown blood samples both with and without the protein being assayed present therein.
4656033 ISOLATED, SOLUBLE IMMUNOGEN AGAINST SCHISTOSOMA MANSONI AND A METHOD OF VACCINATION EMPLOYING SAME Stephanie L James, Alan Sher assigned to The United States of America as represented by the Department of Health and Human Services The present invention discloses a method of vaccination against Schistosoma mansoni employing membrane-free, soluble immunogens. It has been found that the route of administering the vaccine and fortification thereof with a suitable adjuvant are quite critical in eliciting immunity in the host.
4656025 QUANTITATIVE SCREENING ASSAY OF TUMOR GLOBULIN FROM GASTRIC JUICE
4656131 METHOD FOR PRODUCING INTERFERONS
Emmanue Deutsch A quantitative screening protocol for the assay of tumor globulin which is separated from gastric juice of human patients at risk for gastric cancer comprising a purification procedure by column chromatography to recover tumor globulin from the gastric aspirate followed by a quantitative detection procedure in which the tumor globulin antigen from gastric juice which has been enriched in the separation process. The enriched tumor globulin is tested by ELISA quantitative assay using avidin/biotin/horseradish peroxidase. The preferred column is in the form of agarose beads covalently linked to both of diethylaminoethyl groups and Cibacron Blue F3GA. The protocol of the invention provides an improved screening of at risk patients to obtain early diagnosis of gastric cancer which is an improvement of applican£3 s prior U.S. Pat. No. 4,219,539 granted Aug. 26, 1980. The sensitivity of the diagnostic method of applic a n t + 3 s aforesaid U.S. Pat. No. 4,219,539 is adequate to detect 250 nanograms/ml of gastric cancer juice antigen per ml of sample. The present ELISA method detects -°.s little as 0.2 nanograms per ml of tumor lgG and suprisingly gastric cancer samples are at a higher level than in patients having normal gastric juice, e.g. varying from about 4 to about 33 nanograms of IgG per mg of protein which is concentrated by column chromatography. + RE
Kazuaki Kitano, Shiger Fujimoto, Sakai, Japan assigned to Takeda Chemical Industries Ltd A method for producing high yields of interferons which comprises cultivating a microorganism carrying an expression vector with a structural gene of interferon inserted therein in a chemically defined medium containing Lglutamic acid and an iron ion source and preferably further containing an zinc ion source or zinc and copper ion sources, and recovering interferon from the culture.
4656253 MONOCLONAL ANTIBODIES AGAINST ALVEOLAR SURFACTANT PROTEIN Joh Lewicki assigned to California Biotechology Inc Monoclonal antibodies specific for a protein associated with a lung surfactant complex, decreased levels of which are related to respiratory distress syndrome, are disclosed. The antibodies are useful for prediction and diagnosis of respiratory problems in newborns.
PATENT ABSTRACTS pie is then mixed with a radioactively labeled sample of the protein being assayed and an antibody capable of immunoreactivity with the protein, the mixture is incubated, the antibody bound protein is separated from the unbound protein and the relative amounts of antibody bound and unbound protein are determined. Then, the antibody mixing, incubation and separation steps are separately repeated with an unknown blood sample from which the protein being assayed has been substantially removed to provide information for the calculation of a corrected protein level. The amount of the protein being assayed is then determined from the reference standard and the separate determinations made with the unknown blood samples both with and without the protein being assayed present therein.
4656033 ISOLATED, SOLUBLE IMMUNOGEN AGAINST SCHISTOSOMA MANSONI AND A METHOD OF VACCINATION EMPLOYING SAME Stephanie L James, Alan Sher assigned to The United States of America as represented by the Department of Health and Human Services The present invention discloses a method of vaccination against Schistosoma mansoni employing membrane-free, soluble immunogens. It has been found that the route of administering the vaccine and fortification thereof with a suitable adjuvant are quite critical in eliciting immunity in the host.
4656025 QUANTITATIVE SCREENING ASSAY OF TUMOR GLOBULIN FROM GASTRIC JUICE
4656131 METHOD FOR PRODUCING INTERFERONS
Emmanue Deutsch A quantitative screening protocol for the assay of tumor globulin which is separated from gastric juice of human patients at risk for gastric cancer comprising a purification procedure by column chromatography to recover tumor globulin from the gastric aspirate followed by a quantitative detection procedure in which the tumor globulin antigen from gastric juice which has been enriched in the separation process. The enriched tumor globulin is tested by ELISA quantitative assay using avidin/biotin/horseradish peroxidase. The preferred column is in the form of agarose beads covalently linked to both of diethylaminoethyl groups and Cibacron Blue F3GA. The protocol of the invention provides an improved screening of at risk patients to obtain early diagnosis of gastric cancer which is an improvement of applican£3 s prior U.S. Pat. No. 4,219,539 granted Aug. 26, 1980. The sensitivity of the diagnostic method of applic a n t + 3 s aforesaid U.S. Pat. No. 4,219,539 is adequate to detect 250 nanograms/ml of gastric cancer juice antigen per ml of sample. The present ELISA method detects -°.s little as 0.2 nanograms per ml of tumor lgG and suprisingly gastric cancer samples are at a higher level than in patients having normal gastric juice, e.g. varying from about 4 to about 33 nanograms of IgG per mg of protein which is concentrated by column chromatography. + RE
Kazuaki Kitano, Shiger Fujimoto, Sakai, Japan assigned to Takeda Chemical Industries Ltd A method for producing high yields of interferons which comprises cultivating a microorganism carrying an expression vector with a structural gene of interferon inserted therein in a chemically defined medium containing Lglutamic acid and an iron ion source and preferably further containing an zinc ion source or zinc and copper ion sources, and recovering interferon from the culture.
4656253 MONOCLONAL ANTIBODIES AGAINST ALVEOLAR SURFACTANT PROTEIN Joh Lewicki assigned to California Biotechology Inc Monoclonal antibodies specific for a protein associated with a lung surfactant complex, decreased levels of which are related to respiratory distress syndrome, are disclosed. The antibodies are useful for prediction and diagnosis of respiratory problems in newborns.