- Email: [email protected]
4668387 Deep submergence rotating biological contactor apparatus
336
PATENT ABSTRACTS
control element to be substantially the same as the temperature of the medium; the electrical circuit in operation produces heat and due to its intimate physical contact with the means for receiving the seeded culture growth medium supplements the heating of the medium by the heater and the supplementation reduces the total energy required to be supplied by the energy source to cultivate the microorganisms below that which would be required were the electrical circuitry not to be in intimate physical contact with the culture growth receiving means; and optical means are included to provide an external indication of the growth of the microorganisms within the interior of the assembly.
4666855 RAPID METHOD FOR DETERMINING THE ISOELECTRIC POINT FOR AMPHOTERIC MOLECULES Victor Yang, Robert S Langer assigned to Massachusetts Institute of Technology A rapid method for determining the isoelectric point for an amphoteric molecule of interest is provided which utilizes the pH-dependent binding atfinity of the molecule for an ionexchange material. The empirically derived pI values are within a range of 0.2 pH units or less of the reported values in the literature and may be more precisely determined by employing a narrower pH gradient as part of the procedure. The unique methodology allows isoelectric point determinations to be accomplished within one hour£3 s time, avoids carrier ampholyte interaction or artifact formation, and allows the determination to be performed at any desired temperature with decreased risk of denaturation.
4666864 GEL COMPOSITION FOR DETERMINING THE FIBRINOGEN CONTENT IN P L A S M A BY ELECTROIMMUNODIFFUSION Paolo Fabrizi, Alessandro Provvedi. Paolo Tarli, Monteriggioni, Italy assigned to Sclavo S pA A composition for determining the fibrinogen content in plasma by electroimmunodiffusion,
said composition essentially comprising heparin and agarose.
4668363 IMMUNOFIXATION ELECTROPHORESIS PROCESS Michael D Gebott, William A Gurske, Joan A Macy assigned to Beckman Instruments Inc A method comprising (a) applying a sample to at least two application areas on an electrophoretic gel; (b) electrophoresing the gel; (c) aligning a template onto the electrophoresed gel, the template having a template slot corresponding to each electrophoresed area; (d) applying a composition capable of fixing proteins in situ to at least one template slot and applying an antisera capable of reacting with one protein to at least one of the remaining template slots; (e) incubating the resultant product of step (d); (O removing the template from the incubated, electrophoresed gel; (g) washing the incubated electrophoresed gel of step (f); (h) drying the washed gel of step (g); (i) staining the dryed gel of step (h); (j) destaining the stained gel of step (i): (k) drying the destained gel of step (j); and (1) analyzing the dryed gel of step (k).
4668387 DEEP SUBMERGENCE ROTATING BIOLOGICAL CONTACTOR APPARATUS Richard L Davie, Donald Gass assigned to Envirex Inc A wastewater treatment apparatus is disclosed in which a rotating biological contactor rotatably mounted on a horizontal shaft is deeply submerged in a wastewater tank, so that a substantial portion of attached biomass does not come into contact with the ambient air. A plurality of gas conduits are disposed in the tank beneath the rotating contactor and are positioned to provide rotational energy to the contactor, to serve as the principal source of oxygen for the submerged biomass and to regulate the rotational velocity of the biological contactor so that the oxygen containing air bubbles are caused to rise into the central region of media rather than being swept along and confined to the outer radius of the media as it rotates.
PATENT ABSTRACTS
control element to be substantially the same as the temperature of the medium; the electrical circuit in operation produces heat and due to its intimate physical contact with the means for receiving the seeded culture growth medium supplements the heating of the medium by the heater and the supplementation reduces the total energy required to be supplied by the energy source to cultivate the microorganisms below that which would be required were the electrical circuitry not to be in intimate physical contact with the culture growth receiving means; and optical means are included to provide an external indication of the growth of the microorganisms within the interior of the assembly.
4666855 RAPID METHOD FOR DETERMINING THE ISOELECTRIC POINT FOR AMPHOTERIC MOLECULES Victor Yang, Robert S Langer assigned to Massachusetts Institute of Technology A rapid method for determining the isoelectric point for an amphoteric molecule of interest is provided which utilizes the pH-dependent binding atfinity of the molecule for an ionexchange material. The empirically derived pI values are within a range of 0.2 pH units or less of the reported values in the literature and may be more precisely determined by employing a narrower pH gradient as part of the procedure. The unique methodology allows isoelectric point determinations to be accomplished within one hour£3 s time, avoids carrier ampholyte interaction or artifact formation, and allows the determination to be performed at any desired temperature with decreased risk of denaturation.
4666864 GEL COMPOSITION FOR DETERMINING THE FIBRINOGEN CONTENT IN P L A S M A BY ELECTROIMMUNODIFFUSION Paolo Fabrizi, Alessandro Provvedi. Paolo Tarli, Monteriggioni, Italy assigned to Sclavo S pA A composition for determining the fibrinogen content in plasma by electroimmunodiffusion,
said composition essentially comprising heparin and agarose.
4668363 IMMUNOFIXATION ELECTROPHORESIS PROCESS Michael D Gebott, William A Gurske, Joan A Macy assigned to Beckman Instruments Inc A method comprising (a) applying a sample to at least two application areas on an electrophoretic gel; (b) electrophoresing the gel; (c) aligning a template onto the electrophoresed gel, the template having a template slot corresponding to each electrophoresed area; (d) applying a composition capable of fixing proteins in situ to at least one template slot and applying an antisera capable of reacting with one protein to at least one of the remaining template slots; (e) incubating the resultant product of step (d); (O removing the template from the incubated, electrophoresed gel; (g) washing the incubated electrophoresed gel of step (f); (h) drying the washed gel of step (g); (i) staining the dryed gel of step (h); (j) destaining the stained gel of step (i): (k) drying the destained gel of step (j); and (1) analyzing the dryed gel of step (k).
4668387 DEEP SUBMERGENCE ROTATING BIOLOGICAL CONTACTOR APPARATUS Richard L Davie, Donald Gass assigned to Envirex Inc A wastewater treatment apparatus is disclosed in which a rotating biological contactor rotatably mounted on a horizontal shaft is deeply submerged in a wastewater tank, so that a substantial portion of attached biomass does not come into contact with the ambient air. A plurality of gas conduits are disposed in the tank beneath the rotating contactor and are positioned to provide rotational energy to the contactor, to serve as the principal source of oxygen for the submerged biomass and to regulate the rotational velocity of the biological contactor so that the oxygen containing air bubbles are caused to rise into the central region of media rather than being swept along and confined to the outer radius of the media as it rotates.