4668620 Reducing background interference activity in enzyme-label immunoassays

4668620 Reducing background interference activity in enzyme-label immunoassays

PATENT ABSTRACTS nicotinamide adenine dinucleotide phosphate in the presence of malate dehydrogenase to obtain the corresponding reduced product. The ...

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PATENT ABSTRACTS nicotinamide adenine dinucleotide phosphate in the presence of malate dehydrogenase to obtain the corresponding reduced product. The reaction is preferably carried out under reduced pressure, while removing carbon dioxide, formed during the reaction, from the reaction system.

4665016 CHROMOGENIC PROCESS PREPARATION

COMPOUNDS, A FOR THEIR AND THEIR USE

Helmut Heber, Reinhard Eberle, Volker Teetz, Marburg, Federal Republic Of Germany assigned to Behringwerke AktiengeseUschaft Chromogenic compounds of the formula I See Patent for Chemical Structure (I) and a process for their preparation are described, X denoting a hydrogen atom, a protective group customarily used in peptide chemistry or a group which irreversibly blocks the terminal amino group, A and P being identical or different and denoting an alpha-, beta- or gamma-amino acid which is composed of 2 to 15 carbon atoms with up to 3 nitrogen atoms, 2 sulfur atoms and 6 oxygen atoms, the side-chain of which can be substituted, and P also denoting a dipeptide formed from amino acids of these types, B denoting arginine, homoarginine, lysine, homolysine, ornithine or histidine and R denoting a substituent in the 2- or 3-position, The compounds can be used as substrates for the direction and the quantitative determination of hydrolytic enzymes of enzyme class 3.4.21.

4665028 METHOD FOR PRODUCTION OF AN IMMOBILIZED ENZYME PREPARATION BY MEANS OF A CROSSLINKING AGENT

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4665034 DEVICE FOR PERFORMING QUALITATIVE ENZYME IMMUNOASSAYS Howard M Chandler, Mississauga, Canada assigned to Allelix Inc The invention relates to a device and method for performing qualitative enzyme immunoassays. The device comprises at least one tube having antibody, antigen or hapten attached to an internal surface thereof. A first syringe is connectable to a first end of the tube or tubes and supplies a flow of test liquid therethrough. A second syringe having a two piston arrangement is connected to the tube so as to allow a first wash solution followed by an enzyme conjugate solution to be flowed therethrough. Finally a third syringe, also having a two piston configuration, is connected to the tube so as to allow a second wash solution followed by an enzyme substrate and metabolite indicator solution to be flowed therethrough.

4666832 PYRUVATE

OXIDASE

Erich Elstner, Karl-Heinz Schleifer, Friedrich Gotz, Barbara Sedewitz, Albert Roder, Hans Mollering, Han Seidel, Gr Federal Republic Of Germana assigned to Boehringer Mannheim GmbH The present invention provides a new pyruvate oxidase which decarboxylates pyruvate with the formation of hydrogen peroxide, characterized in that it is active without the addition of FAD, TPP and divalent metal ions. The present invention also provides a process for preparing this new enzyme and a reagent containing it.

Shmue Amotz. Mdenmara assigned to Novo lndustri A/S

4668620

A method for production of an immobilized enzyme by means of a crosslinking agent wherein the following components are brought together in aqueous medium: (a) an enzyme preparation: (b) a crosslinking agent: and (c) an inert water soluble salt in a relatively high concentration. The salt hinders solubility of the enzyme in the medium, yet the enzyme is fully accessible to the crosslinking agent. High enzyme activity recovery may be obtained.

REDUCING BACKGROUND INTERFERENCE ACTIVITY IN ENZYME-LABEL IMMUNOASSAYS Richar Armenta, fan Gibbons, John Olson assigned to Syntex (U S A ) lnc Background interference which disrupts the measurement of an analyte in enzyme immuno-