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4675295 Process for producing subculturable lymphokine-producing human T cell hybridomas
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PATENT ABSTRACTS
4675295 PROCESS FOR PRODUCING SUBCULTURABLE LYMPHOKINE-PRODUCING HUMAN T CELL HYBRIDOMAS Toshiaki Osawa, Yoshiro Kobayashi, Makoto Asada, Masahiro Higuchi, Tokyo. Japan assigned to Denki Kagaku Kogyo Kabushiki Kaisha A process for preparing human T cell hybridomas which are subculturable and produce lymphokines comprising the steps of: (1) treating a human acute leukemia cell with a protein and/or RNA synthesis inhibitor: (2) independently activating a human T cell with a mitogen or antigen; (3) fusing the thus-treated human acute leukemia cell with the thusactivated human T cell in the presence of a fusion accelerator: and (4) isolating the thus-formed hybridoma. An in vivo process for producing lymphokines using the hybridomas is also described. 4675297 GENES ENCODING BOVINE PROLACTIN John D Baxter, Walter L Miller, Joseph A Martial assigned to The Regents of the University of California
logical specimens is described. The technique involves denaturation of cytoplasmic preparations, followed by dotting of up to 96 samples onto a single sheet of nitrocellulose, hybridization with a 32P-labeled cDNA plasmid, autoradiography, and scanning. By analyzing cytoplasmic preparations instead of purified RNA. manipulations of multiple sampies prior to analysis are minimized. Experiments with a clonal line of rat pituitary tumor (GH3) cells show that this technique can be employed to follow the induction by Ca2£30 + 0 + L of prolactin mRNA sequences, employing cytoplasm prepared from as little as 2.5+33 10+ HU 4 + L cells. The specificity of the technique for prolactin mRNA is shown by employing GC cells, a GH + HD 3 + L cell variant lacking detectable prolactin mRNA sequences. Experiments with cultured rat hemipituitaries show that the prolactin mRNA present in cytoplasm corresponding to as little as 1/100 of a pituitary can be readily detected. This technique is quite simple, can be quantified, and permits the +PG,2 simultaneous analysis of multiple samples while requiring very small amounts of material for analysis. Hence, it should be quite useful for example for studies with various experimental systems of the regulation of specific mRNA levels. 4678751 HYBRID HUMAN LEUKOCYTE INTERFERONS David Goeddel assigned to Genentech lnc
A DNA sequence encoding bovine prolactin and optionally including condons for the preceding 10 amino acids is used to construct expression systems to obtain recombinant production of these proteins.
4677054 METHOD FOR SIMPLE ANALYSIS OF RELATIVE NUCLEIC ACID LEVELS IN M U L T I P L E S M A L L S A M P L E S BY CYTOPLASMIC DOT HYBRIDIZATION Bruce A White, F Carter Bancroft assigne0 to Sloan-Kettering Institute for Cancer Research A simple technique for the simultaneous measurement of relative levels of a specific mRNA in numberous small samples of bio-
Disclosed herein are methods and means of microbially preparing novel human hybrid leukocyte interferons, useful in the treatment of viral and neoplastic diseases, by DNA recombination of parental interferon genes, taking advantage of common restriction endonuclease cleavage sites therein and in carrier expression plasmids.
4680176 DELETION MUTANT OF A HERPESVIRUS AND VACCINE CONTAINING SAID VIRUS Antonius J Berns, Arnold L Gielkens, Grave, Netherlands assigned to Centraal Diergeneeskundig Instituut The invention relates to live deletion mutants of a herpesvirus, especially of Pseudorabies virus.
PATENT ABSTRACTS
4675295 PROCESS FOR PRODUCING SUBCULTURABLE LYMPHOKINE-PRODUCING HUMAN T CELL HYBRIDOMAS Toshiaki Osawa, Yoshiro Kobayashi, Makoto Asada, Masahiro Higuchi, Tokyo. Japan assigned to Denki Kagaku Kogyo Kabushiki Kaisha A process for preparing human T cell hybridomas which are subculturable and produce lymphokines comprising the steps of: (1) treating a human acute leukemia cell with a protein and/or RNA synthesis inhibitor: (2) independently activating a human T cell with a mitogen or antigen; (3) fusing the thus-treated human acute leukemia cell with the thusactivated human T cell in the presence of a fusion accelerator: and (4) isolating the thus-formed hybridoma. An in vivo process for producing lymphokines using the hybridomas is also described. 4675297 GENES ENCODING BOVINE PROLACTIN John D Baxter, Walter L Miller, Joseph A Martial assigned to The Regents of the University of California
logical specimens is described. The technique involves denaturation of cytoplasmic preparations, followed by dotting of up to 96 samples onto a single sheet of nitrocellulose, hybridization with a 32P-labeled cDNA plasmid, autoradiography, and scanning. By analyzing cytoplasmic preparations instead of purified RNA. manipulations of multiple sampies prior to analysis are minimized. Experiments with a clonal line of rat pituitary tumor (GH3) cells show that this technique can be employed to follow the induction by Ca2£30 + 0 + L of prolactin mRNA sequences, employing cytoplasm prepared from as little as 2.5+33 10+ HU 4 + L cells. The specificity of the technique for prolactin mRNA is shown by employing GC cells, a GH + HD 3 + L cell variant lacking detectable prolactin mRNA sequences. Experiments with cultured rat hemipituitaries show that the prolactin mRNA present in cytoplasm corresponding to as little as 1/100 of a pituitary can be readily detected. This technique is quite simple, can be quantified, and permits the +PG,2 simultaneous analysis of multiple samples while requiring very small amounts of material for analysis. Hence, it should be quite useful for example for studies with various experimental systems of the regulation of specific mRNA levels. 4678751 HYBRID HUMAN LEUKOCYTE INTERFERONS David Goeddel assigned to Genentech lnc
A DNA sequence encoding bovine prolactin and optionally including condons for the preceding 10 amino acids is used to construct expression systems to obtain recombinant production of these proteins.
4677054 METHOD FOR SIMPLE ANALYSIS OF RELATIVE NUCLEIC ACID LEVELS IN M U L T I P L E S M A L L S A M P L E S BY CYTOPLASMIC DOT HYBRIDIZATION Bruce A White, F Carter Bancroft assigne0 to Sloan-Kettering Institute for Cancer Research A simple technique for the simultaneous measurement of relative levels of a specific mRNA in numberous small samples of bio-
Disclosed herein are methods and means of microbially preparing novel human hybrid leukocyte interferons, useful in the treatment of viral and neoplastic diseases, by DNA recombination of parental interferon genes, taking advantage of common restriction endonuclease cleavage sites therein and in carrier expression plasmids.
4680176 DELETION MUTANT OF A HERPESVIRUS AND VACCINE CONTAINING SAID VIRUS Antonius J Berns, Arnold L Gielkens, Grave, Netherlands assigned to Centraal Diergeneeskundig Instituut The invention relates to live deletion mutants of a herpesvirus, especially of Pseudorabies virus.