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4693971 Highly sensitive enzyme assay method
PATENT ABSTRACTS the alcohol concentration in the fermenting broth at a desirable concentration on the basis of a difference between a measured alcohol concentration in the fermenting broth and a desirable alcohol concentration and the alcohol consumption rate calculated during each of the cycles by the control computer; and then the feeding apparatus is operated to control the feed rate of the alcohol solution responsive to a control signal generated by the control computer as a result of the aforementioned calculations, to thereby controllably feed alcohol solution as raw material to the fermenting broth.
4692417 IMMUNOLOGICAL IDENTIFICATION TEST FOR GROUP D STREPTOCOCCI USING ACHROMOPEPTIDASE Martyn F Webster, Basingstoke, United Kingdom assigned to Oxoid Limited The grouping of a sample of a streptococcus is identified by treating the sample with achromopeptidase prior to contacting the sample with a group-specific antibody bound to minute, insoluble carrier particles. A positive identification is indicated by agglutination of the carrier particles.
4~25~ RADIOACTIVE LABELING OF PROTEINS WITH NUCLEOSIDES OR NUCLEOTIDES Nanibhushan Dattagupta assigned to Molecular Diagnostics Inc A radioactively labeled protein comprising a protein, and a radioactive nucleoside or nucleotide, the protein being covalently linked to the nucleoside or nucleotide. Advantageously the linkage is through an NH2 group of the protein and through a carbonyl group of a ringopened sugar moiety of the nucleoside or nucleotide. The protein can be insulin, an immunoglobulin or protein A. The radioactive moiety may be a P, C, S, H, I or Hg atom. The labels can be used to indicate the presence and amount of the protein in a biological assay.
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469~ AGENT FOR QUANTITATIVE DETERMINATION OF MICROORGANISMS Tsunehiro Kitagawa, Nagasaki, Japan assigned to Dainippon Pharmaceutical Co Ltd An agent for quantitative determination of microorganisms, which comprises (a) an antibody prepared from a strain of the same species as the species of microorganisms to be determined, (b) an insolubilized cell component of a strain which can sufficiently bind to the antibody (a) but does not release and replace to other strains which have been bound with the antibody (a), and (c) a labelled second antibody, and a method for the quantitative determination of microorganisms. The agent and method of the present invention are useful for determinating simultaneously not only a strain from which the antibody is prepared but also other strains of the same species which are contained in the test sample.
4693970 IMMUNOASSAY USING COMPLEMENT Connell James 0£3, Randal A Hoke, Patrick D Mize assigned to Becton Dickinson and Company A method for immunoassay of an analyte in a fluid includes contacting the analyte with an antianalyte to give a bound fraction. The bound fraction activates the first component of complement whereby a substrate present in the fluid is modulated to provide a detectable signal. The signal may be detected to establish the presence or absence of the analyte in the fluid, or it may be quantitatively measured to determine the concentration of the analyte in the fluid. The invention includes a kit of materials useful in performing the method of the invention.
4693971 HIGHLY SENSITIVE ENZYME ASSAY METHOD Hideo Misaki, Shizuoka, Japan assigned to Toyo Jozo Kabushiki Kaisha
86
PATENT ABSTRACTS
A highly sensitive quantitative assay method for any one component which is L-glycero-3phosphate (G3P), dihydroxyacetone-3phosphate (DHAP), nicotinamide adenine dinucleotide (NAD) or reduced NAD, in a specimen to be assayed, comprising causing this component in the specimen to take part in the cycling reaction See Patent for Chemical Structure wherein GPO is glycerophosphate oxidase and GPDH is glycerophosphate dehydrogenase, and measuring a detectable change in the reaction system. There is thus provided a novel G3PGHAP cycling reaction using GPO, which consumes 0 2 and generates H202 and DHAP, with a substrate of G3P, and furthermore GPDH which consumes reduced NAD and generates N A D and G3P, with a substrate of DHAP. Examples of specimens are specimens which contain any one ofG3P, DHAP, NAD or reduced NAD, or which liberate or generate such a component. By proceeding at a rate of more than ten cycles per minute and measuring the amount of a detectable change in the reaction, the component in a specimen can easily and sensitively be detected with good accuracy.
4693983 REACTOR FOR CULTIVATING BIOLOGICAL MATERIAL SUCH AS IMMOBILIZED CELLS Graham A Davies, Ferd Mavituna, Macclesfield, United Kingdom assigned to National Research Development Corporation A reactor 10 for cultivating biological material is colonized with plant cells 3 in channels 2a of a support matrix. The biological material cannot pass out of the channels. Each colonized channel adjoins several non-colonized channels 2, viz. a nutrient supply channel, an extractant channel, a heat-transfer channel and a gas supply channel. The walls separating the different pairs of these channels are permeable to the relevant material only.
4695539 PROCESS FOR QUANTITATIVE DETERMINATION OF SUBSTRATE TREATED WITH OXIDASE
4693972 COMPOSITION AND METHOD FOR RAPID DETECTION OF MICROORGANISMS IN CLINICAL SAMPLES James Mansour, Thomas H Schulte, Vernon Neece assigned to Becton Dickinson and Company A method for rapid detection of microorganisms in a body fluid sample includes detecting the microorganisms after treatment of the sample with a lysing agent in order to dissolve sample components other than microorganisms, and staining with a fluorescent dye. The lysing agent may be part of a composition which includes a culture medium thereby providing simultaneous lysis and growth before staining. Detection is preferably accomplished by reliance on the fluorescence emitted by the dye having been properly excited by light energy. A composition suitable for use in the above-described method includes a growth medium and a lysing agent.
Yoshitsug Sakata, Yoshibonu Miyashita, Tadashi Hamanaka, Hiroyuki Kodera, Yutaka Miki, Kazuhiko Yamanishi, Toshire Hanada, Otsu, Japan assigned to Wako Pure Chemical Industries Ltd A substrate in a sample can be determined quantitatively by measuring colorimetrically superoxide ion generated by treating the sample with a specific oxidase corresponding to the substrate to be determined, said oxidase treatment and measuring of the generated superoxide ion being conducted by using a reagent composition comprising (a) an oxidase, (b) peroxidase, (c) a phenol and/or an amine, (d) a thiol compound, (e) a color producing reagent to be reduced, and if necessary (f) a chelating agent.
4695540 QUANTITATIVE DETERMINATION OF SUBSTRATE TREATED WITH OXIDASE Kazuhiko Yamanishi, Toshiro Hanada, Tokyo, Japan assigned to Wako Pure Chemical Industries Ltd
4692417 IMMUNOLOGICAL IDENTIFICATION TEST FOR GROUP D STREPTOCOCCI USING ACHROMOPEPTIDASE Martyn F Webster, Basingstoke, United Kingdom assigned to Oxoid Limited The grouping of a sample of a streptococcus is identified by treating the sample with achromopeptidase prior to contacting the sample with a group-specific antibody bound to minute, insoluble carrier particles. A positive identification is indicated by agglutination of the carrier particles.
4~25~ RADIOACTIVE LABELING OF PROTEINS WITH NUCLEOSIDES OR NUCLEOTIDES Nanibhushan Dattagupta assigned to Molecular Diagnostics Inc A radioactively labeled protein comprising a protein, and a radioactive nucleoside or nucleotide, the protein being covalently linked to the nucleoside or nucleotide. Advantageously the linkage is through an NH2 group of the protein and through a carbonyl group of a ringopened sugar moiety of the nucleoside or nucleotide. The protein can be insulin, an immunoglobulin or protein A. The radioactive moiety may be a P, C, S, H, I or Hg atom. The labels can be used to indicate the presence and amount of the protein in a biological assay.
85
469~ AGENT FOR QUANTITATIVE DETERMINATION OF MICROORGANISMS Tsunehiro Kitagawa, Nagasaki, Japan assigned to Dainippon Pharmaceutical Co Ltd An agent for quantitative determination of microorganisms, which comprises (a) an antibody prepared from a strain of the same species as the species of microorganisms to be determined, (b) an insolubilized cell component of a strain which can sufficiently bind to the antibody (a) but does not release and replace to other strains which have been bound with the antibody (a), and (c) a labelled second antibody, and a method for the quantitative determination of microorganisms. The agent and method of the present invention are useful for determinating simultaneously not only a strain from which the antibody is prepared but also other strains of the same species which are contained in the test sample.
4693970 IMMUNOASSAY USING COMPLEMENT Connell James 0£3, Randal A Hoke, Patrick D Mize assigned to Becton Dickinson and Company A method for immunoassay of an analyte in a fluid includes contacting the analyte with an antianalyte to give a bound fraction. The bound fraction activates the first component of complement whereby a substrate present in the fluid is modulated to provide a detectable signal. The signal may be detected to establish the presence or absence of the analyte in the fluid, or it may be quantitatively measured to determine the concentration of the analyte in the fluid. The invention includes a kit of materials useful in performing the method of the invention.
4693971 HIGHLY SENSITIVE ENZYME ASSAY METHOD Hideo Misaki, Shizuoka, Japan assigned to Toyo Jozo Kabushiki Kaisha
86
PATENT ABSTRACTS
A highly sensitive quantitative assay method for any one component which is L-glycero-3phosphate (G3P), dihydroxyacetone-3phosphate (DHAP), nicotinamide adenine dinucleotide (NAD) or reduced NAD, in a specimen to be assayed, comprising causing this component in the specimen to take part in the cycling reaction See Patent for Chemical Structure wherein GPO is glycerophosphate oxidase and GPDH is glycerophosphate dehydrogenase, and measuring a detectable change in the reaction system. There is thus provided a novel G3PGHAP cycling reaction using GPO, which consumes 0 2 and generates H202 and DHAP, with a substrate of G3P, and furthermore GPDH which consumes reduced NAD and generates N A D and G3P, with a substrate of DHAP. Examples of specimens are specimens which contain any one ofG3P, DHAP, NAD or reduced NAD, or which liberate or generate such a component. By proceeding at a rate of more than ten cycles per minute and measuring the amount of a detectable change in the reaction, the component in a specimen can easily and sensitively be detected with good accuracy.
4693983 REACTOR FOR CULTIVATING BIOLOGICAL MATERIAL SUCH AS IMMOBILIZED CELLS Graham A Davies, Ferd Mavituna, Macclesfield, United Kingdom assigned to National Research Development Corporation A reactor 10 for cultivating biological material is colonized with plant cells 3 in channels 2a of a support matrix. The biological material cannot pass out of the channels. Each colonized channel adjoins several non-colonized channels 2, viz. a nutrient supply channel, an extractant channel, a heat-transfer channel and a gas supply channel. The walls separating the different pairs of these channels are permeable to the relevant material only.
4695539 PROCESS FOR QUANTITATIVE DETERMINATION OF SUBSTRATE TREATED WITH OXIDASE
4693972 COMPOSITION AND METHOD FOR RAPID DETECTION OF MICROORGANISMS IN CLINICAL SAMPLES James Mansour, Thomas H Schulte, Vernon Neece assigned to Becton Dickinson and Company A method for rapid detection of microorganisms in a body fluid sample includes detecting the microorganisms after treatment of the sample with a lysing agent in order to dissolve sample components other than microorganisms, and staining with a fluorescent dye. The lysing agent may be part of a composition which includes a culture medium thereby providing simultaneous lysis and growth before staining. Detection is preferably accomplished by reliance on the fluorescence emitted by the dye having been properly excited by light energy. A composition suitable for use in the above-described method includes a growth medium and a lysing agent.
Yoshitsug Sakata, Yoshibonu Miyashita, Tadashi Hamanaka, Hiroyuki Kodera, Yutaka Miki, Kazuhiko Yamanishi, Toshire Hanada, Otsu, Japan assigned to Wako Pure Chemical Industries Ltd A substrate in a sample can be determined quantitatively by measuring colorimetrically superoxide ion generated by treating the sample with a specific oxidase corresponding to the substrate to be determined, said oxidase treatment and measuring of the generated superoxide ion being conducted by using a reagent composition comprising (a) an oxidase, (b) peroxidase, (c) a phenol and/or an amine, (d) a thiol compound, (e) a color producing reagent to be reduced, and if necessary (f) a chelating agent.
4695540 QUANTITATIVE DETERMINATION OF SUBSTRATE TREATED WITH OXIDASE Kazuhiko Yamanishi, Toshiro Hanada, Tokyo, Japan assigned to Wako Pure Chemical Industries Ltd