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4703005 Expression vector carrying a gene coding for a phosphate-binding protein, a method for preparing the same and a method for preparing the same and a method for producing a polypeptide using the same
PATENT ABSTRACTS
4703005 EXPRESSION VECTOR CARRYING A GENE CODING FOR A PHOSPHATE-BINDING PROTEIN, A METHOD FOR PREPARING THE SAME AND A METHOD FOR PREPARING THE SAME AND A METHOD FOR PRODUCING A POLYPEPTIDE USING THE SAME Atsu Nakata, Hideo Shinagawa, Toyonaka, Japan assigned to The Research Foundation for Microbial Diseases of Osaka University An expression vector carrying a gene coding for a phosphate-binding protein has been found to have a strong gene expression. The expression vector can be produced by transforming a bacterium belonging to Enterobacteriaceae with a recombinant vector carrying a D N A fragment containing a gene coding for a phosphatebinding protein to form transformants, selecting the transformants containing the desired recombinant vector from said transformants, and isolating the desired recombinant vector from the selected transformants. The expression vector is useful for producing polypeptides.
4703008 DNA SEQUENCES ENCODING ERYTHROPOIETIN Fu-Kuen Lin assigned to Kiren-Amgen Inc Disclosed are novel polypeptides possessing part or all of the primary structural conformation and one or more of the biological properties of mammalian erythropoietin ( EPO ) which are characterized in preferred forms by being the product of procaryotic or eucaryotic host expression of an exogenous DNA sequence. IIhistratively, genomic DNA, cDNA and manufactured DNA sequences coding for part or all of the sequence of amino acid residues of EPO or for analogs thereof are incorporated into autonomously replicating plasmid or viral vectors employed to transform or transfect suitable procaryotic or eucaryotic host cells such as bacteria, yeast or vertebrate cells in culture. Upon isolation from culture media or cellular lysates or fragments, products of expression of the D N A sequences display, e.g., the immunological properties and in vitro and in vivo biological activities of EPO of human or monkey species orig-
223
ins. Disclosed also are chemically synthesized polypeptides sharing the biochemical and immunological properties of EPO. Also disclosed are improved methods for the detection of specific single stranded polynucleotides in a beterologns cellular or viral sample prepared from, e.g., DNA present in a plasmid or viralborne cDNA or genomic D N A library.
4703009 RDNA CLONING VECTOR PVEI, DELETION AND HYBRID MUTANTS AND RECOMBINANT DERIVATIVES THEREOF PRODUCTS AND PROCESSES Tanya MacNeil, Patrice H Gibbons assigned to Merck & Co Inc Novel plasmid pVE1, deletion mutants thereof, recombinant derivatives thereof, which is the same as the genome or nucleic acid of such plasmids and derivatives of such genome, which are useful as recombinant DNA cloning vectors into host organisms, such as bacteria, for example, Streptomyces avermitilis; portions of such plasmid genome are additionally useful as adjuncts in recombinant DNA cloning procedures, for examples: 1. to permit the maintenance of cloned DNA in the host, either in an integrated state or as an autonomous element; 2. to serve as promoters for increasing expression of endogenous or foreign genes wherein said promoters are ligated to such genes or otherwise serve as promoters; and 3. to serve as regulatory elements for achieving control over endogenous and foreign gene expression; as cloning vectors, pVE1 its deletion mutants, and other derivatives serve for the amplification and transfer of DNA sequences (genes) coding for useful functions, such modified cloning vectors are introduced into the recipient organism by conjugation or transformation; wherein the hybrid DNA functions in an integrated mode and/or in a plasmid mode.
4703011 THYMIDINE KINASE DELETION MUTANTS OF BOVINE HERPESVIRUS-I Malon Kit, Saul Kit assigned to NovaGene Inc; Baylor College of Medici Bovine herpesvirus type 1 (infectious bovine rhinotracheitis virus) mutants which fail to pro-
4703005 EXPRESSION VECTOR CARRYING A GENE CODING FOR A PHOSPHATE-BINDING PROTEIN, A METHOD FOR PREPARING THE SAME AND A METHOD FOR PREPARING THE SAME AND A METHOD FOR PRODUCING A POLYPEPTIDE USING THE SAME Atsu Nakata, Hideo Shinagawa, Toyonaka, Japan assigned to The Research Foundation for Microbial Diseases of Osaka University An expression vector carrying a gene coding for a phosphate-binding protein has been found to have a strong gene expression. The expression vector can be produced by transforming a bacterium belonging to Enterobacteriaceae with a recombinant vector carrying a D N A fragment containing a gene coding for a phosphatebinding protein to form transformants, selecting the transformants containing the desired recombinant vector from said transformants, and isolating the desired recombinant vector from the selected transformants. The expression vector is useful for producing polypeptides.
4703008 DNA SEQUENCES ENCODING ERYTHROPOIETIN Fu-Kuen Lin assigned to Kiren-Amgen Inc Disclosed are novel polypeptides possessing part or all of the primary structural conformation and one or more of the biological properties of mammalian erythropoietin ( EPO ) which are characterized in preferred forms by being the product of procaryotic or eucaryotic host expression of an exogenous DNA sequence. IIhistratively, genomic DNA, cDNA and manufactured DNA sequences coding for part or all of the sequence of amino acid residues of EPO or for analogs thereof are incorporated into autonomously replicating plasmid or viral vectors employed to transform or transfect suitable procaryotic or eucaryotic host cells such as bacteria, yeast or vertebrate cells in culture. Upon isolation from culture media or cellular lysates or fragments, products of expression of the D N A sequences display, e.g., the immunological properties and in vitro and in vivo biological activities of EPO of human or monkey species orig-
223
ins. Disclosed also are chemically synthesized polypeptides sharing the biochemical and immunological properties of EPO. Also disclosed are improved methods for the detection of specific single stranded polynucleotides in a beterologns cellular or viral sample prepared from, e.g., DNA present in a plasmid or viralborne cDNA or genomic D N A library.
4703009 RDNA CLONING VECTOR PVEI, DELETION AND HYBRID MUTANTS AND RECOMBINANT DERIVATIVES THEREOF PRODUCTS AND PROCESSES Tanya MacNeil, Patrice H Gibbons assigned to Merck & Co Inc Novel plasmid pVE1, deletion mutants thereof, recombinant derivatives thereof, which is the same as the genome or nucleic acid of such plasmids and derivatives of such genome, which are useful as recombinant DNA cloning vectors into host organisms, such as bacteria, for example, Streptomyces avermitilis; portions of such plasmid genome are additionally useful as adjuncts in recombinant DNA cloning procedures, for examples: 1. to permit the maintenance of cloned DNA in the host, either in an integrated state or as an autonomous element; 2. to serve as promoters for increasing expression of endogenous or foreign genes wherein said promoters are ligated to such genes or otherwise serve as promoters; and 3. to serve as regulatory elements for achieving control over endogenous and foreign gene expression; as cloning vectors, pVE1 its deletion mutants, and other derivatives serve for the amplification and transfer of DNA sequences (genes) coding for useful functions, such modified cloning vectors are introduced into the recipient organism by conjugation or transformation; wherein the hybrid DNA functions in an integrated mode and/or in a plasmid mode.
4703011 THYMIDINE KINASE DELETION MUTANTS OF BOVINE HERPESVIRUS-I Malon Kit, Saul Kit assigned to NovaGene Inc; Baylor College of Medici Bovine herpesvirus type 1 (infectious bovine rhinotracheitis virus) mutants which fail to pro-