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4711843 Method and vector organism for controlled accumulation of cloned heterologous gene products in bacillus subtilis
PATENT ABSTRACTS 4710566 PURIFICATION OF THE MESSENGER RNA CAP-BINDING PROTEIN
231
produced by the method of the invention are useful for increasing the secretion of heterologous gene products produced by transformed host organisms. The invention further discloses a method for producing recombinant DNA sequences in vivo.
John W Kozarich, Robert E Rhoads assigned to Yale University The synthesis of a new affinity medium, the paminophenylgamma-ester of 7methylguanosine-5'-triphosphate coupled to Sepharose, for use in affinity chromatography is claimed. An improved method in terms of speed and purity for the isolation of messenger ribonucleic acid cap-binding protein is claimed. The synthesis of the p-aminophenyl- gamma-ester of 7-methylguanosine-5'-triphosphate is described. 4711843 METHOD AND VECTOR ORGANISM FOR CONTROLLED ACCUMULATION OF CLONED HETEROLOGOUS GENE PRODUCTS IN BACILLUS SUBTILIS Shing Chang assigned to Cetus Corporation A method and a cloning vector are described for the controlled accumulation of cloned heterologous gene products in Bacillus subtilis. The cloning vector is capable of being replicated in B. subtilis and includes the heterologous gene located and oriented such as to be under the control of an operator, promoter, and ribosomal binding site sequence. The gene codes for a protein which is under the control of a transport mechanism by which the protein is secreted by the B. subtilis. The gene product is recovered from the growth medium for the B. subtilis. The cloning vector is also capable of similar use in other bacteria such as E. coli. 4711844 MODIFIED
SIGNAL PEPTIDES
Shing Chang assigned to Cetus Corporation The invention discloses a method for producing modified signal peptides sequences derived from wild-type signal peptide sequences of the type that are capable of forming membrane-bound lipoproteins. Modified signal peptide sequences
~11845 PORTABLE TEMPERATURESENSITIVE CONTROL CASSETTE David H Gelfand, Frances C Lawyer assigned to Cetus Corporation Two types of convenient portable control cassettes for the expression of protein encoding sequences in proearyotes are disclosed. Both cassettes comprise the PL promoter from lambda phage, which is controllable by means of a temperature sensitive repressor, operably linked to the ribosome binding site for N-gene (NRBS). In one embodiment, this cassette is bordered by restriction sites upstream of the PL promoter and immediately downstream from the NRBS permitting the insertion of a desired sequence containing its own start codon downstream from the cassette. The other embodiment contains an ATG start codon within the cassette and has a restriction site immediately 3' of the start codon.
4711847 PREPARATION
OF SECRETIN
Wolfgang Konig, Joachim Engels, Eugen Uhlmann, Waldemar Wetekam, Hofbeim am Taunus, Federal Republic Of Germany assigned to 501 Hoechst Aktiengesellschaft Secretin, which cannot be prepared directly by genetic engineering because of its carboxylic acid carboxyl-terminus, can be obtained by preparing secretylglycine by genetic engineering and then obtaining secretin therefrom by enzymatic conversion of the terminal glycine radical. The gene for the secretylglycine is synthesized chemically from smaller single-stranded units which are linked enzymatically to give the complete gene, incorporated into a suitable vector and amplified therein, after which the peptide is isolated directly or as a fusion protein and, after cyanogen bromide cleavage, is converted enzymatically into secretin.
231
produced by the method of the invention are useful for increasing the secretion of heterologous gene products produced by transformed host organisms. The invention further discloses a method for producing recombinant DNA sequences in vivo.
John W Kozarich, Robert E Rhoads assigned to Yale University The synthesis of a new affinity medium, the paminophenylgamma-ester of 7methylguanosine-5'-triphosphate coupled to Sepharose, for use in affinity chromatography is claimed. An improved method in terms of speed and purity for the isolation of messenger ribonucleic acid cap-binding protein is claimed. The synthesis of the p-aminophenyl- gamma-ester of 7-methylguanosine-5'-triphosphate is described. 4711843 METHOD AND VECTOR ORGANISM FOR CONTROLLED ACCUMULATION OF CLONED HETEROLOGOUS GENE PRODUCTS IN BACILLUS SUBTILIS Shing Chang assigned to Cetus Corporation A method and a cloning vector are described for the controlled accumulation of cloned heterologous gene products in Bacillus subtilis. The cloning vector is capable of being replicated in B. subtilis and includes the heterologous gene located and oriented such as to be under the control of an operator, promoter, and ribosomal binding site sequence. The gene codes for a protein which is under the control of a transport mechanism by which the protein is secreted by the B. subtilis. The gene product is recovered from the growth medium for the B. subtilis. The cloning vector is also capable of similar use in other bacteria such as E. coli. 4711844 MODIFIED
SIGNAL PEPTIDES
Shing Chang assigned to Cetus Corporation The invention discloses a method for producing modified signal peptides sequences derived from wild-type signal peptide sequences of the type that are capable of forming membrane-bound lipoproteins. Modified signal peptide sequences
~11845 PORTABLE TEMPERATURESENSITIVE CONTROL CASSETTE David H Gelfand, Frances C Lawyer assigned to Cetus Corporation Two types of convenient portable control cassettes for the expression of protein encoding sequences in proearyotes are disclosed. Both cassettes comprise the PL promoter from lambda phage, which is controllable by means of a temperature sensitive repressor, operably linked to the ribosome binding site for N-gene (NRBS). In one embodiment, this cassette is bordered by restriction sites upstream of the PL promoter and immediately downstream from the NRBS permitting the insertion of a desired sequence containing its own start codon downstream from the cassette. The other embodiment contains an ATG start codon within the cassette and has a restriction site immediately 3' of the start codon.
4711847 PREPARATION
OF SECRETIN
Wolfgang Konig, Joachim Engels, Eugen Uhlmann, Waldemar Wetekam, Hofbeim am Taunus, Federal Republic Of Germany assigned to 501 Hoechst Aktiengesellschaft Secretin, which cannot be prepared directly by genetic engineering because of its carboxylic acid carboxyl-terminus, can be obtained by preparing secretylglycine by genetic engineering and then obtaining secretin therefrom by enzymatic conversion of the terminal glycine radical. The gene for the secretylglycine is synthesized chemically from smaller single-stranded units which are linked enzymatically to give the complete gene, incorporated into a suitable vector and amplified therein, after which the peptide is isolated directly or as a fusion protein and, after cyanogen bromide cleavage, is converted enzymatically into secretin.