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4713345 Fermentation methods and apparatus
92
PATENT ABSTRACTS
mechanism for stirring an egg white solution and an ion-exchange resin, having a strainer provided in a lower portion thereof, having liquid discharge pipes connected thereto at a position below the strainer, preferably at the bottom of the tank, and at the side wall above the strainer at a height such that the resin when left at rest will not overflow, respectively, and further having a resin discharge pipe connected thereto at the lower portion of the side wall, above and in the vicinity of the strainer; a positive displacement pump connected to the liquid discharge pipes for transferring the egg white solution after removal of lysozyme to a reservoir tank; and a centrifugal pump connected to the resin discharge pipe for transferring the mixture of the resin and water to a separate tank, whereby collection by adsorption can be made simple, convenient for use and also suitable for a smallquantity treatment.
4713328 MICROBIAL
ENZYME ASSAYS
Robert Yolken A method is disclosed for efficient, rapid diagnosis of bacterial infections by measuring for the presence of beta-lactamase using 14Cbenzylpenicillin as an enzyme substrate and isolating cleaved substrate with an ion exchange column.
4713344 HAND OPERATED PRESS FOR TISSUE EXTRACTION Albert Markhart assigned to Regents of the University of Minnesota
4713327 DETERMINATION OF TOTAL CREATINE KINASE OR AN ISOENZYME WITH A MULTILAYER ANALYTICAL ELEMENT John B Findlay, Annie Wu, Gary E Norton assigned to Eastman Kodak Company A multilayer analytical element for the determination ofcreatine kinase comprises a support having thereon, in order and in fluid contact, a registration layer and an isotropically porous spreading layer. The registration layer contains a binder material at a coverage of at least about 8 g/m2, which coverage improves the stability and keeping properties of the element in high humidity environments. This element is useful in an analytical method for determinin~ total creatine kinase or any one of its isoenzymes, e.g. creatine kinase-MB.
A hand operated press is used for extracting cell sap from biological tissue, such as from leaf tissue of plants, as well as from animal tissue as a removable barrel that holds a suitable screen in place in a well. The barrel houses a plunger that is of small diameter so that when the barrel has tissue added to it the plunger is inserted and a substantial pressure may be exerted manually to insure rupturing the cell membranes and releasing all of the cell liquid (in plants called sap) for analysis. In the preferred form the screen holds at least one standard size filter paper disk in place so that the liquid is first forced through the screen to retain large solid particles, and the filter paper disk is wetted for use in a standard analyzer for direct analysis of the desired characteristics. The press is portable, easy to use and disassemble for cleaning, and reduces the time required for determining characteristics of a cell, such as its osmotic potential, and also reduces the chance of the sap becoming altered by environmental factors.
4713345 FERMENTATION METHODS APPARATUS
AND
Steven L Ramsden assigned to Grain Processing Corporation
PATENT ABSTRACTS Fermentation reactions are conducted by introducing into a fermentation vessel a flocculating microorganism and a fermentable medium and continually withdrawing fermented broth from the vessel through a conduit which is disposed at an angle of at least 60 degrees but less than 90 degrees from the horizontal.
93 4713351
MONOCLONAL ANTIBODY DIRECTED TO AN ANTIGEN DERIVED FROM HUMAN OVARIAN TUMORS AND A RADIOIMMUNOASSAY USING THE ANTIBODY Suzanne Knauf assigned to The University of Rochester
4713349 TEMPLET FOR SIMULTANEOUS SCREENING OF SEVERAL ANTIBODIES AND METHOD OF USING THE SAME Andrew Levin assigned to Wisconsin Alumni Research Foundation A templet (10) is provided having a lower plate (11) which receives a carrier sheet having antigens immobilized thereon as transferred from an electrophoresis gel. An upper plate (12) having a plurality of parallel channels (22) formed therein, is placed over the lower plate such that the channels lie over the carrier sheet. The two plates (11, 12) are then pressed tightly together, as by engagement with screws (16) at the periphery of the plates. A bridge bar (28) extends over the central portion of the plate and pressure screws (30) are mounted to the bridge bar in position to engage and apply pressure to the top surface of the upper plate (12). Various antibody containing liquids are introduced into the various channels through holes (25) extending to the ends of the channels; the solutions are allowed to remain in contact with the antigens on the carrier sheet for a period of time, after which the liquid is withdrawn from the channels, and the plates separated. The entire carrier sheet is subsequently exposed to tracer antibodies labeled radioactively or otherwise. The carrier sheet so treated shows a series of parallel vertical stripes, in each of which those horizontal antigen bands reactive with the particular antibody applied to the stripe are indicated. An antigen, or mixture of antigens, may thus be screened with many different antibodies simultaneously, and the user may compare the relative positions of antibody-labeled antigen bands on a single cartier sheet.
A monoclonal antibody directed to an antigen, named NB/70K, derived from human ovarian carcinomas and a radioimmunoassay for the detection of ovarian carcinomas using said monoclonal antibody.
4714613 METHOD OF SUPPRESSING CELL GROWTH BY IMMUNOTHERAPY Daniel Shouval, David A Shafritz, Jack Wands, Jerusalem, NY, Israel assigned to The Albert Einstein College of Medicine of Yeshiva University; The General Hospital Corporati A selective method of suppressing the growth of cells which express a viral antigen on the surface thereof, which comprises administering to the cells a growth suppressing amount of a monoclonal antibody against said viral antigen, especially a method of suppressing the growth of hepatocytes or hepatoma cells persistently infected with HBsAg which comprises administering to the cells a growth suppressing or lethal amount of a complement fixing monoclonal IgM or IgG2a antibody against HBsAg.
4714672 IMMUNOASSAYS INVOLVING COMPLEMENT LYSING OF CHROMOPHORE CONTAINING MICROCAPSULES Kyuji Rokugawa, Yasuko Tamayama, Ootawara, Japan assigned to Kabushiki Kaisha Toshiba An immunoassay of the type which utilizes the hemolysis of microcapsules such as red blood cells. Microcapsules which can be lysed by corn-
PATENT ABSTRACTS
mechanism for stirring an egg white solution and an ion-exchange resin, having a strainer provided in a lower portion thereof, having liquid discharge pipes connected thereto at a position below the strainer, preferably at the bottom of the tank, and at the side wall above the strainer at a height such that the resin when left at rest will not overflow, respectively, and further having a resin discharge pipe connected thereto at the lower portion of the side wall, above and in the vicinity of the strainer; a positive displacement pump connected to the liquid discharge pipes for transferring the egg white solution after removal of lysozyme to a reservoir tank; and a centrifugal pump connected to the resin discharge pipe for transferring the mixture of the resin and water to a separate tank, whereby collection by adsorption can be made simple, convenient for use and also suitable for a smallquantity treatment.
4713328 MICROBIAL
ENZYME ASSAYS
Robert Yolken A method is disclosed for efficient, rapid diagnosis of bacterial infections by measuring for the presence of beta-lactamase using 14Cbenzylpenicillin as an enzyme substrate and isolating cleaved substrate with an ion exchange column.
4713344 HAND OPERATED PRESS FOR TISSUE EXTRACTION Albert Markhart assigned to Regents of the University of Minnesota
4713327 DETERMINATION OF TOTAL CREATINE KINASE OR AN ISOENZYME WITH A MULTILAYER ANALYTICAL ELEMENT John B Findlay, Annie Wu, Gary E Norton assigned to Eastman Kodak Company A multilayer analytical element for the determination ofcreatine kinase comprises a support having thereon, in order and in fluid contact, a registration layer and an isotropically porous spreading layer. The registration layer contains a binder material at a coverage of at least about 8 g/m2, which coverage improves the stability and keeping properties of the element in high humidity environments. This element is useful in an analytical method for determinin~ total creatine kinase or any one of its isoenzymes, e.g. creatine kinase-MB.
A hand operated press is used for extracting cell sap from biological tissue, such as from leaf tissue of plants, as well as from animal tissue as a removable barrel that holds a suitable screen in place in a well. The barrel houses a plunger that is of small diameter so that when the barrel has tissue added to it the plunger is inserted and a substantial pressure may be exerted manually to insure rupturing the cell membranes and releasing all of the cell liquid (in plants called sap) for analysis. In the preferred form the screen holds at least one standard size filter paper disk in place so that the liquid is first forced through the screen to retain large solid particles, and the filter paper disk is wetted for use in a standard analyzer for direct analysis of the desired characteristics. The press is portable, easy to use and disassemble for cleaning, and reduces the time required for determining characteristics of a cell, such as its osmotic potential, and also reduces the chance of the sap becoming altered by environmental factors.
4713345 FERMENTATION METHODS APPARATUS
AND
Steven L Ramsden assigned to Grain Processing Corporation
PATENT ABSTRACTS Fermentation reactions are conducted by introducing into a fermentation vessel a flocculating microorganism and a fermentable medium and continually withdrawing fermented broth from the vessel through a conduit which is disposed at an angle of at least 60 degrees but less than 90 degrees from the horizontal.
93 4713351
MONOCLONAL ANTIBODY DIRECTED TO AN ANTIGEN DERIVED FROM HUMAN OVARIAN TUMORS AND A RADIOIMMUNOASSAY USING THE ANTIBODY Suzanne Knauf assigned to The University of Rochester
4713349 TEMPLET FOR SIMULTANEOUS SCREENING OF SEVERAL ANTIBODIES AND METHOD OF USING THE SAME Andrew Levin assigned to Wisconsin Alumni Research Foundation A templet (10) is provided having a lower plate (11) which receives a carrier sheet having antigens immobilized thereon as transferred from an electrophoresis gel. An upper plate (12) having a plurality of parallel channels (22) formed therein, is placed over the lower plate such that the channels lie over the carrier sheet. The two plates (11, 12) are then pressed tightly together, as by engagement with screws (16) at the periphery of the plates. A bridge bar (28) extends over the central portion of the plate and pressure screws (30) are mounted to the bridge bar in position to engage and apply pressure to the top surface of the upper plate (12). Various antibody containing liquids are introduced into the various channels through holes (25) extending to the ends of the channels; the solutions are allowed to remain in contact with the antigens on the carrier sheet for a period of time, after which the liquid is withdrawn from the channels, and the plates separated. The entire carrier sheet is subsequently exposed to tracer antibodies labeled radioactively or otherwise. The carrier sheet so treated shows a series of parallel vertical stripes, in each of which those horizontal antigen bands reactive with the particular antibody applied to the stripe are indicated. An antigen, or mixture of antigens, may thus be screened with many different antibodies simultaneously, and the user may compare the relative positions of antibody-labeled antigen bands on a single cartier sheet.
A monoclonal antibody directed to an antigen, named NB/70K, derived from human ovarian carcinomas and a radioimmunoassay for the detection of ovarian carcinomas using said monoclonal antibody.
4714613 METHOD OF SUPPRESSING CELL GROWTH BY IMMUNOTHERAPY Daniel Shouval, David A Shafritz, Jack Wands, Jerusalem, NY, Israel assigned to The Albert Einstein College of Medicine of Yeshiva University; The General Hospital Corporati A selective method of suppressing the growth of cells which express a viral antigen on the surface thereof, which comprises administering to the cells a growth suppressing amount of a monoclonal antibody against said viral antigen, especially a method of suppressing the growth of hepatocytes or hepatoma cells persistently infected with HBsAg which comprises administering to the cells a growth suppressing or lethal amount of a complement fixing monoclonal IgM or IgG2a antibody against HBsAg.
4714672 IMMUNOASSAYS INVOLVING COMPLEMENT LYSING OF CHROMOPHORE CONTAINING MICROCAPSULES Kyuji Rokugawa, Yasuko Tamayama, Ootawara, Japan assigned to Kabushiki Kaisha Toshiba An immunoassay of the type which utilizes the hemolysis of microcapsules such as red blood cells. Microcapsules which can be lysed by corn-