- Email: [email protected]
4757008 Enzyme immobilization in a macroporous non-ionic resin
Enzymes
and
Enzyme
Systems
4757012
4753881
ASCORBIC ACID INTERMEDIATES AND PROCESS ENZYMES
PURIFIED ENZYME AND PROCESS THEREFOR Wu-Kuang Yeh, Joe E Dotzlaf assigned to Eli Lilly and Company
David A Estell, Robert Lazarus, David R Light, Jeffrey Miller, William H Rastetter assigned to Genentech Inc
Deacetoxycephalosporin C synthetase is provided in purified form via chromatography of crude cell-free extracts over a weak anion exchange resin followed by gel filtration and hydroxylapatite chromatography, all carried out in the presence of glycerol, a C 1-C3 alkyl monohydric alcohol, e.g., ethanol, a sulfhydryl containing reducing agent, e.g., dithiothreitol, and ascorbate. The purified enzyme which possesses both expandase and hydroxylase activities can be further purified by chromatography over a strong anion exchange resin.
Recombinant plasmids containing the gene encoding 2,5-diketogluconic acid reductase are prepared and used to transform microorganisms. 2,5,DKG reductase is expressed by the microorganisms.
4758514 ASCORBIC ACID INTERMEDIATES AND PROCESS ENZYMES
4757008
David R Light, William H Rastetter assigned to Genentech Inc
ENZYME IMMOBILIZATION IN A MACROPOROUS NON-IONIC RESIN
2-keto-L-gluonic acid (2-KLG) is prepared from 2,5-diketo-D-gluconic acid (2,5-DKG) using a microorganism containing the enzyme 2,5-DKG reductase produced by an expression plasmid encoding a gene for the enzyme. The product, 2K L G is a precursor for the synthesis of ascorbic acid (vitamin C).
Lawrence F Reverman assigned to Miles Inc Enzyme-containing microbial cells are contacted with a macro-porous non-ionic resin under reduced pressure below atmospheric to cause the cells to become incorporated into pores of the resin. The resin is then contacted with a multi-functional, amine reactive material to immobilize the cells within the pores. Prior to contacting with the resin, cells may be disrupted to produce a system containing cell fragments, intact cells and solubilized enzyme. A preferred enzyme is glucose isomerase.
4760024 IMMOBILIZATION
OF ENZYMES
Oreste J Lantero assigned to Miles Inc 289
and
Enzyme
Systems
4757012
4753881
ASCORBIC ACID INTERMEDIATES AND PROCESS ENZYMES
PURIFIED ENZYME AND PROCESS THEREFOR Wu-Kuang Yeh, Joe E Dotzlaf assigned to Eli Lilly and Company
David A Estell, Robert Lazarus, David R Light, Jeffrey Miller, William H Rastetter assigned to Genentech Inc
Deacetoxycephalosporin C synthetase is provided in purified form via chromatography of crude cell-free extracts over a weak anion exchange resin followed by gel filtration and hydroxylapatite chromatography, all carried out in the presence of glycerol, a C 1-C3 alkyl monohydric alcohol, e.g., ethanol, a sulfhydryl containing reducing agent, e.g., dithiothreitol, and ascorbate. The purified enzyme which possesses both expandase and hydroxylase activities can be further purified by chromatography over a strong anion exchange resin.
Recombinant plasmids containing the gene encoding 2,5-diketogluconic acid reductase are prepared and used to transform microorganisms. 2,5,DKG reductase is expressed by the microorganisms.
4758514 ASCORBIC ACID INTERMEDIATES AND PROCESS ENZYMES
4757008
David R Light, William H Rastetter assigned to Genentech Inc
ENZYME IMMOBILIZATION IN A MACROPOROUS NON-IONIC RESIN
2-keto-L-gluonic acid (2-KLG) is prepared from 2,5-diketo-D-gluconic acid (2,5-DKG) using a microorganism containing the enzyme 2,5-DKG reductase produced by an expression plasmid encoding a gene for the enzyme. The product, 2K L G is a precursor for the synthesis of ascorbic acid (vitamin C).
Lawrence F Reverman assigned to Miles Inc Enzyme-containing microbial cells are contacted with a macro-porous non-ionic resin under reduced pressure below atmospheric to cause the cells to become incorporated into pores of the resin. The resin is then contacted with a multi-functional, amine reactive material to immobilize the cells within the pores. Prior to contacting with the resin, cells may be disrupted to produce a system containing cell fragments, intact cells and solubilized enzyme. A preferred enzyme is glucose isomerase.
4760024 IMMOBILIZATION
OF ENZYMES
Oreste J Lantero assigned to Miles Inc 289