4762707 New conjugates associating, by covalent bond, an enzyme with an antibody, and medicinal associations using the said conjugates

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PATENT ABSTRACTS

An enzyme producing microorganism or an enzyme produced thereby is immobilized by contacting the microorganism or enzyme with polyethylenimine and adding chitosan and glutaraldehyde to form a reaction product. In a preferred embodiment, the reaction product is extruded onto the revolving plate of a spheronizing device to form spherical enzyme aggregates having enhanced physical and biocatalytic properties. 4762707 NEW CONJUGATES ASSOCIATING, BY COVALENT BOND, AN ENZYME WITH AN ANTIBODY, AND MEDICINAL ASSOCIATIONS USING THE SAID CONJUGATES Franz Jansen, Pierre Gros, St Mathieu De Treviers, France assigned to Sanofi o(Societe Anonyme) The present invention relates to new immunoenzymic conjugates resulting from a chemical coupling, by covalent bond, of an antibody or a fragment of antibody, which has retained the capacity of recognizing the selected antigen, with an enzyme capable of producing ammonium ions from natural substrates which are well tolerated in higher animal organisms. The invention also relates to a process for the preparation of these conjugates and the medicinal associations of said conjugates with an immunotoxin.

4762788 PROCESS FOR PRODUCING CELLULOLYTIC ENZYMES Miche Warzywoda, FerreVeronique , Jacques Pourquie, Rueil Malmaison, France assigned to Institut Francais du Petrole This invention concerns a process of producing cellulolytic enzymes by fermentation with the Trichoderma reesei fungus. This process includes two steps: (a) a first step of subjecting to aerobian fermentation a culture medium comprising a strain of Trichoderma reesei, nutrition compounds and a small quantity of cellulose and sugar, (b) a second step ofcontining the aerobian fermentation by adding soluble sugar at a speed such that the sugar concentration in the fermentation zone remains under 0.3% by weight. The

cellulolytic enzymes obtained are used in the enzymatic hydrolysis of a lignocellulosic biomass. 4764466 METHOD FOR STABILIZING AN IMMOBILIZED FIBRINOLYTIC ENZYME Katsuhik Suyama, Yasunori Yabushita, Masanao Koyama, Kunihiko Takagi, Kyoto, Japan assigned to Unitika Ltd A fibrinolytic enzyme immobilized on a carrier is stabilized by treating the surface of the carrier on which the enzyme is immobilized with a basic amino acid. The amino acid is preferably histidine, arginine or lysine. Carriers may be inorganic materials, natural polymeric materials or synthetic polymeric materials, in a variety of forms. Fibrinolytic enzymes immobilized include plasmin, brinase, urokinase, streptokinase and tissue plasminogen activator. The stabilized immobilized fibrinolytic enzyme maintains activity better during storage or sterilization. 4769173 ENZYMATIC DETERGENT AND BLEACHING COMPOSITION Johannes M Cornelissen, Jan Klugkist, Cornelis A Lagerwaard, Ton Swarthoff, David Thom, Vlaardingen, Netherlands assigned to Lever Brothers Company The invention relates to the use of a certain class of lipases together with strong bleaching agents in detergent compositions. This class of lipases consists of fungal lipases cx Humicola lanuginosa or Thermomyces lanuginosus, and bacterial lipases which show a positive immunological cross-reaction with the antibody of the lipase produced by Chromobacter viscosum var. lipolyticum N R R L B-3673. The strong bleaching agents are stronger than the sodium perborate/TAED system, i.e. stronger than peracetic acid or they yield, on perhydrolysis, a peracid faster than the sodium perborate/TAED system. 4772686 ENZYME

INHIBITION

Michae Szelke, David Jones, Ruislip, United Kingdom assigned to Aktiebolaget Hassle