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4772553 Method for determination of enzyme activity
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PATENT ABSTRACTS alcohol as the substance which accelerates the enzymatic cleavage of the amino acid ester bond or peptide ester bond of component (a), characterized in that it contains a salt as the accelerating substance.
4760025 MODIFIED ENZYMES AND METHODS FOR MAKING SAME David Estell, James Wells assigned to Genencor Inc A cloned subtilisin gene has been modified at specific sites to cause amino acid substitutions at certain spots in the enzyme. The modified enzyme, preferably produced by Bacillus, is useful in combination with detergents.
4760067
are absorbed with virus-infected cell extracts to remove intertypic cross-reacting antibodies. 4764464 SEMISYNTHETIC ENZYMES: APOPROTEINS FROM HEME PROTEINS AS HYDROLASES Hay Zemel assigned to Allied-Signal Inc Proteins having a hydrophobic cavity proximate to which is a residue promoting hydrolysis of functional moieties are effective semisynthetic hydrolases. Heme proteins from which the heme has been removed usually have at least one imidazole residue proximate to the cavity, and thus act as quite effective esterases. Because the size and shape of the cavities of such proteins are capable of broad diversity a wide spectrum of substrates may be hydrolyzed by these semisynthetic enzymes.
ALLYLSULFOXIDE ENZYME INHIBITORS Raymond Firestone assigned to Merck & Co Inc Organic sulfoxides having a latent allyl group bound to the sulfur are enzyme inhibitors of the suicide or Kcat type. 4772553 4764459 ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR DETERMINING ANTI-BODIES AGAINST HERPES SIMPLEX VIRUS (HSV) TYPES 1 AND 2 IN HUMAN SERA Berge Hampar, Stephen Showalter, Martin Zweig assigned to The United States of America as represented by the Department of Health and Human Services A method and test kit for the serological diagnosis of human infection by herpes sipmlex virus type I (HSV-I) and herpes simplex virus type 2 (HSV-2) using immunoatfinity purified virus-coded glycoproteins as target antigens. A preferred embodiment of the method employs a variation of the enzyme-linked immunosorbent assay.(ELISA) whereby monoclonal antibodies are used to purify target antigens, and test sera
METHOD FOR DETERMINATION OF ENZYME ACTIVITY Setsuro Fujii, Satosh Sugiyama, Syouzou Sawai, Toyonaka, Japan assigned to Torii & Co Inc This invention relates to a method for determining an enzyme-, an enzyme inhibitor-, an enzyme activator-, or zymogen-activity, which comprises allowing the enzyme to react with a substrate of the formula See Patent for Chemical Structure (I) (wherein RI represents an amino acid of an oligopeptide combined in the form of ester through the C-terminal carboxyl group of said amino acid or oligop~eptide and R2 represents a hydrogen or bromine atom) to hydrolyze the substrate, then allowing the hydrolysis product of the formula See Patent for Chemical Structure to react with a FR-ITR salt (Fast Red-ITR salt (N, N'-diethyl-4methoxymetanilamide diazonium salt)) to form a pigment, and determining the pigment.
PATENT ABSTRACTS alcohol as the substance which accelerates the enzymatic cleavage of the amino acid ester bond or peptide ester bond of component (a), characterized in that it contains a salt as the accelerating substance.
4760025 MODIFIED ENZYMES AND METHODS FOR MAKING SAME David Estell, James Wells assigned to Genencor Inc A cloned subtilisin gene has been modified at specific sites to cause amino acid substitutions at certain spots in the enzyme. The modified enzyme, preferably produced by Bacillus, is useful in combination with detergents.
4760067
are absorbed with virus-infected cell extracts to remove intertypic cross-reacting antibodies. 4764464 SEMISYNTHETIC ENZYMES: APOPROTEINS FROM HEME PROTEINS AS HYDROLASES Hay Zemel assigned to Allied-Signal Inc Proteins having a hydrophobic cavity proximate to which is a residue promoting hydrolysis of functional moieties are effective semisynthetic hydrolases. Heme proteins from which the heme has been removed usually have at least one imidazole residue proximate to the cavity, and thus act as quite effective esterases. Because the size and shape of the cavities of such proteins are capable of broad diversity a wide spectrum of substrates may be hydrolyzed by these semisynthetic enzymes.
ALLYLSULFOXIDE ENZYME INHIBITORS Raymond Firestone assigned to Merck & Co Inc Organic sulfoxides having a latent allyl group bound to the sulfur are enzyme inhibitors of the suicide or Kcat type. 4772553 4764459 ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR DETERMINING ANTI-BODIES AGAINST HERPES SIMPLEX VIRUS (HSV) TYPES 1 AND 2 IN HUMAN SERA Berge Hampar, Stephen Showalter, Martin Zweig assigned to The United States of America as represented by the Department of Health and Human Services A method and test kit for the serological diagnosis of human infection by herpes sipmlex virus type I (HSV-I) and herpes simplex virus type 2 (HSV-2) using immunoatfinity purified virus-coded glycoproteins as target antigens. A preferred embodiment of the method employs a variation of the enzyme-linked immunosorbent assay.(ELISA) whereby monoclonal antibodies are used to purify target antigens, and test sera
METHOD FOR DETERMINATION OF ENZYME ACTIVITY Setsuro Fujii, Satosh Sugiyama, Syouzou Sawai, Toyonaka, Japan assigned to Torii & Co Inc This invention relates to a method for determining an enzyme-, an enzyme inhibitor-, an enzyme activator-, or zymogen-activity, which comprises allowing the enzyme to react with a substrate of the formula See Patent for Chemical Structure (I) (wherein RI represents an amino acid of an oligopeptide combined in the form of ester through the C-terminal carboxyl group of said amino acid or oligop~eptide and R2 represents a hydrogen or bromine atom) to hydrolyze the substrate, then allowing the hydrolysis product of the formula See Patent for Chemical Structure to react with a FR-ITR salt (Fast Red-ITR salt (N, N'-diethyl-4methoxymetanilamide diazonium salt)) to form a pigment, and determining the pigment.