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48 Hours
CHAPTER
5
48 HOURS Sagittal Sections
Figure 5.1 1
4 3
2
Figure 5.2
5
1
4 3
2
Figure 5.3
1
6 4 3
2
1. myotome
2. yolk
3. lens
Atlas of Zebrafish Development. 10.1016/B978-0-12-374016-8.00005-4 © 2012 Elsevier Inc. All rights reserved.
4. optic cup
5. neuromasts
6. yolk extension
33
34
ATLAS OF ZEBRAFISH DEVELOPMENT
Figure 5.4 3
2
1
4 7
6
5
Figure 5.5 2
1
3
4 7
6
5
Figure 5.6 8
2
1
3
9
4 7 6
5
1. pectoral fin bud
2. myotome
3. notochord
4. yolk extension
5. yolk
6. lens
7. optic cup
8. gill arches
9. spinal cord
48 hours
35
Figure 5.7 2
1
3 4
7
6
5
8
9 11
10
Figure 5.8
1
12
14 13
2 3
8
7
6
9 15
11
10
Figure 5.9 14 13 2 12 1
3
7
6
8
9 15
11
10
1. optic tectum 2. medulla oblongata 3. otic vesicle
4. gill arches 5. pectoral fin bud 6. myotome
7. notochord 8. spinal cord 9. yolk extension
10. yolk 11. optic cup 12. tegmentum
13. cerebellum 14. fourth ventricle 15. olfactory epithelium
36
ATLAS OF ZEBRAFISH DEVELOPMENT
Figure 5.10 3
4
5
6
7
2
1
9
8
10 14
13
12
11
Figure 5.11
1
2
5
3 4
15
9
8
7
10 14
13
11
Figure 5.12
1
2
3 4
5
15
9
8
7
10 14 13
17
16 11
1. optic tectum 2. tegmentum 3. cerebellum
4. fourth ventricle 5. medulla oblongata 6. otic vesicle
7. myotome 8. notochord 9. spinal cord
10. yolk extension 11. yolk 12. optic cup
13. anterior commissure 14. forebrain ventricle 15. vertical myoseptum
16. heart 17. hypothalamus
48 hours
37
Figure 5.13 3
4
5
2
6
1
13
7
8
12 11 10
9
Figure 5.14 5
4
14
2
8
13 12 11 10 9
Figure 5.15 4 1
5
2
14 6
7
8
13 12 11
10 9
1. optic tectum 2. tegmentum 3. cerebellum
4. fourth ventricle 5. medulla oblongata 6. notochord
7. spinal cord 8. myotome 9. yolk
10. heart 11. hypothalamus 12. anterior commissure
13. forebrain ventricle 14. intestine
38
ATLAS OF ZEBRAFISH DEVELOPMENT
Figure 5.16 3
2
4
1
5
10 9 8 7 6
Figure 5.17 3
2
4
1
5
10 9 8 7 6
Figure 5.18 3
12 2 1
4 5
11
10
9
8
7 6
1. tegmentum 2. fourth ventricle
3. medulla oblongata 4. liver
5. myotome 6. yolk
7. heart 8. hypothalamus
9. anterior commissure 10. forebrain ventricle
11. optic tectum 12. cerebellum
48 hours
39
Figure 5.19 3
5
4
6
2
1
7
12 11 10
9 8
Figure 5.20 2
3
4
5
7
1
12
6
11
8
Figure 5.21 3
5
1
12
13
8
1. optic tectum 2. tegmentum 3. cerebellum
4. fourth ventricle 5. medulla oblongata 6. liver
7. myotome 8. yolk 9. heart
10. hypothalamus 11. anterior commissure 12. forebrain ventricle
13. olfactory epithelium
40
ATLAS OF ZEBRAFISH DEVELOPMENT
Figure 5.22 2
3
1
5
6
4
Figure 5.23
2
1
3
7 8
5
6
4
Figure 5.24
2
1
3
7 8
5
4
1. optic tectum 2. cerebellum
3. medulla oblongata 4. yolk
5. optic cup 6. olfactory epithelium
7. otic vesicle 8. pectoral fin bud
48 hours
41
Figure 5.25
1 2
5 4
3
Figure 5.26
1 2
5
4
3
Figure 5.27
5
4
3
1. otic vesicle
2. pectoral fin bud
3. yolk
4. optic cup
5. lens
42
ATLAS OF ZEBRAFISH DEVELOPMENT
Coronal Sections Figure 5.28
1
2
Figure 5.29
2
1
3
4
Figure 5.30
1
3
6 5
4
1. medulla oblongata
2. median fin fold
3. cerebellum
4. fourth ventricle
5. spinal cord
6. myotome
48 hours
43
Figure 5.31
1
2
3
2
3
4
5
Figure 5.32
6
1
5
4
7
Figure 5.33
6
1
2
5
1. cerebellum
2. medulla oblongata
3
4
7
3. myotome
4. spinal cord
5. fourth ventricle
6. optic tectum
7. otic vesicle
44
ATLAS OF ZEBRAFISH DEVELOPMENT
Figure 5.34
3
2
1
8
4
5
6
7
Figure 5.35
4
3
2
1
8
9 5
7
Figure 5.36
1
2
8
1. optic tectum 2. cerebellum 3. medulla oblongata
4
3
5
9
7
4. pectoral fin bud 5. myotome 6. spinal cord
7. otic vesicle 8. fourth ventricle 9. notochord
48 hours
45
Figure 5.37
2
1
4
3
6
5
7
Figure 5.38
8
5
3
6
7 9
4
Figure 5.39
5
8
7
1. optic tectum 2. cerebellum
9
6
10
3. medulla oblongata 4. pectoral fin bud
5. myotome 6. notochord
7. forebrain ventricle 8. tegmentum
9. gill arches 10. liver
46
ATLAS OF ZEBRAFISH DEVELOPMENT
Figure 5.40
2
1
3
8
4
6 5
7
Figure 5.41
9
1
3
4 8
6 7
10
2
Figure 5.42
1
9 11 8
6 7
1. tegmentum 2. yolk 3. myotome
4. notochord 5. liver
10
2
6. gill arches 7. optic cup
8. forebrain ventricle 9. median fin fold
10. intestine 11. yolk extension
48 hours
47
Figure 5.43
1
2 3 7
5 6
4
Figure 5.44
4 8
1
3
7
2
5 6
9
Figure 5.45
4
1
8
3
2
7 6
1. tegmentum 2. median fin fold
9
10
3. yolk extension 4. yolk
5. gill arches 6. optic cup
7. forebrain ventricle 8. optic chiasm
9. lens 10. hypothalamus
48
ATLAS OF ZEBRAFISH DEVELOPMENT
Figure 5.46
3
1
2
4
5
6
10 9
87
Figure 5.47
3
1
2 4
10 9
8 7
5
6
Figure 5.48
3 1 2
4
6
10 9
1. tegmentum 2. optic chiasm
8 7
3. yolk 4. yolk extension
5. median fin fold 6. gill arches
7. hypothalamus 8. lens
9. optic cup 10. forebrain ventricle
48 hours
49
Figure 5.49
5 23
1
4 6
7
10 8
9
Figure 5.50
5 2
4
10 8
9
Figure 5.51
5
2
4
10 9
1. tegmentum 2. optic chiasm
3. lens 4. heart
5. yolk 6. yolk extension
7. gill arches 8. hypothalamus
9. optic cup 10. forebrain ventricle
50
ATLAS OF ZEBRAFISH DEVELOPMENT
Figure 5.52
4
2
3
1
6 5
Figure 5.53
4
7
3
1 5
Figure 5.54
4
3
1
1. olfactory bulb 2. optic chiasm
3. heart 4. yolk
5. optic cup
6. forebrain ventricle
7. olfactory pit
48 hours
51
Transverse Sections Figure 5.55
Figure 5.56
4 3 2
1
Figure 5.57
Figure 5.58
5
4
11
5
3
7 10
7
8 9
6
Figure 5.59
Figure 5.60 5
13
13
5
16 15
12 14
1. 2. 3. 4.
forebrain ventricle tectal ventricle tegmentum optic tectum
5. 6. 7. 8.
fourth ventricle olfactory bulb optic cup lens
9. 10. 11. 12.
anterior commissure hypothalamus cerebellum heart
13. 14. 15. 16.
medulla oblongata yolk gill arches otic vesicle
52
ATLAS OF ZEBRAFISH DEVELOPMENT
Figure 5.61
Figure 5.62 1
4
4
1
3 5
2
2
Figure 5.63
Figure 5.64 10
10
6 9
7
6
9
7
8
8
2
2
Figure 5.65
Figure 5.66
10 9
10
6
6
9 11
2
1. fourth ventricle 2. yolk 3. otic vesicle
2
4. medulla oblongata 5. gill arches 6. myotome
7. pectoral fin bud 8. liver 9. notochord
10. spinal cord 11. intestine
48 hours
53
Figure 5.67
Figure 5.68
4
4
1
3
2
2
Figure 5.69
Figure 5.70
4
4
1
3
5
Figure 5.71
Figure 5.72 6
6 4 3
4
1
3
1
5
5
2. yolk
1
3
2
1. myotome
1
3
3. notochord
4. spinal cord
5. yolk extension
6. median fin fold
54
ATLAS OF ZEBRAFISH DEVELOPMENT
Figure 5.73
Figure 5.74 1 5
1 5
2
4
3
3
Figure 5.75
Figure 5.76 1 5
1 5
2
4
2
4 3
3
Figure 5.77
Figure 5.78 1 5 4
1. median fin fold
2
4
2. myotome
1 5 4
2
3. yolk extension
2
4. notochord
5. spinal cord
5
48 HOURS Sagittal Sections
Figure 5.1 1
4 3
2
Figure 5.2
5
1
4 3
2
Figure 5.3
1
6 4 3
2
1. myotome
2. yolk
3. lens
Atlas of Zebrafish Development. 10.1016/B978-0-12-374016-8.00005-4 © 2012 Elsevier Inc. All rights reserved.
4. optic cup
5. neuromasts
6. yolk extension
33
34
ATLAS OF ZEBRAFISH DEVELOPMENT
Figure 5.4 3
2
1
4 7
6
5
Figure 5.5 2
1
3
4 7
6
5
Figure 5.6 8
2
1
3
9
4 7 6
5
1. pectoral fin bud
2. myotome
3. notochord
4. yolk extension
5. yolk
6. lens
7. optic cup
8. gill arches
9. spinal cord
48 hours
35
Figure 5.7 2
1
3 4
7
6
5
8
9 11
10
Figure 5.8
1
12
14 13
2 3
8
7
6
9 15
11
10
Figure 5.9 14 13 2 12 1
3
7
6
8
9 15
11
10
1. optic tectum 2. medulla oblongata 3. otic vesicle
4. gill arches 5. pectoral fin bud 6. myotome
7. notochord 8. spinal cord 9. yolk extension
10. yolk 11. optic cup 12. tegmentum
13. cerebellum 14. fourth ventricle 15. olfactory epithelium
36
ATLAS OF ZEBRAFISH DEVELOPMENT
Figure 5.10 3
4
5
6
7
2
1
9
8
10 14
13
12
11
Figure 5.11
1
2
5
3 4
15
9
8
7
10 14
13
11
Figure 5.12
1
2
3 4
5
15
9
8
7
10 14 13
17
16 11
1. optic tectum 2. tegmentum 3. cerebellum
4. fourth ventricle 5. medulla oblongata 6. otic vesicle
7. myotome 8. notochord 9. spinal cord
10. yolk extension 11. yolk 12. optic cup
13. anterior commissure 14. forebrain ventricle 15. vertical myoseptum
16. heart 17. hypothalamus
48 hours
37
Figure 5.13 3
4
5
2
6
1
13
7
8
12 11 10
9
Figure 5.14 5
4
14
2
8
13 12 11 10 9
Figure 5.15 4 1
5
2
14 6
7
8
13 12 11
10 9
1. optic tectum 2. tegmentum 3. cerebellum
4. fourth ventricle 5. medulla oblongata 6. notochord
7. spinal cord 8. myotome 9. yolk
10. heart 11. hypothalamus 12. anterior commissure
13. forebrain ventricle 14. intestine
38
ATLAS OF ZEBRAFISH DEVELOPMENT
Figure 5.16 3
2
4
1
5
10 9 8 7 6
Figure 5.17 3
2
4
1
5
10 9 8 7 6
Figure 5.18 3
12 2 1
4 5
11
10
9
8
7 6
1. tegmentum 2. fourth ventricle
3. medulla oblongata 4. liver
5. myotome 6. yolk
7. heart 8. hypothalamus
9. anterior commissure 10. forebrain ventricle
11. optic tectum 12. cerebellum
48 hours
39
Figure 5.19 3
5
4
6
2
1
7
12 11 10
9 8
Figure 5.20 2
3
4
5
7
1
12
6
11
8
Figure 5.21 3
5
1
12
13
8
1. optic tectum 2. tegmentum 3. cerebellum
4. fourth ventricle 5. medulla oblongata 6. liver
7. myotome 8. yolk 9. heart
10. hypothalamus 11. anterior commissure 12. forebrain ventricle
13. olfactory epithelium
40
ATLAS OF ZEBRAFISH DEVELOPMENT
Figure 5.22 2
3
1
5
6
4
Figure 5.23
2
1
3
7 8
5
6
4
Figure 5.24
2
1
3
7 8
5
4
1. optic tectum 2. cerebellum
3. medulla oblongata 4. yolk
5. optic cup 6. olfactory epithelium
7. otic vesicle 8. pectoral fin bud
48 hours
41
Figure 5.25
1 2
5 4
3
Figure 5.26
1 2
5
4
3
Figure 5.27
5
4
3
1. otic vesicle
2. pectoral fin bud
3. yolk
4. optic cup
5. lens
42
ATLAS OF ZEBRAFISH DEVELOPMENT
Coronal Sections Figure 5.28
1
2
Figure 5.29
2
1
3
4
Figure 5.30
1
3
6 5
4
1. medulla oblongata
2. median fin fold
3. cerebellum
4. fourth ventricle
5. spinal cord
6. myotome
48 hours
43
Figure 5.31
1
2
3
2
3
4
5
Figure 5.32
6
1
5
4
7
Figure 5.33
6
1
2
5
1. cerebellum
2. medulla oblongata
3
4
7
3. myotome
4. spinal cord
5. fourth ventricle
6. optic tectum
7. otic vesicle
44
ATLAS OF ZEBRAFISH DEVELOPMENT
Figure 5.34
3
2
1
8
4
5
6
7
Figure 5.35
4
3
2
1
8
9 5
7
Figure 5.36
1
2
8
1. optic tectum 2. cerebellum 3. medulla oblongata
4
3
5
9
7
4. pectoral fin bud 5. myotome 6. spinal cord
7. otic vesicle 8. fourth ventricle 9. notochord
48 hours
45
Figure 5.37
2
1
4
3
6
5
7
Figure 5.38
8
5
3
6
7 9
4
Figure 5.39
5
8
7
1. optic tectum 2. cerebellum
9
6
10
3. medulla oblongata 4. pectoral fin bud
5. myotome 6. notochord
7. forebrain ventricle 8. tegmentum
9. gill arches 10. liver
46
ATLAS OF ZEBRAFISH DEVELOPMENT
Figure 5.40
2
1
3
8
4
6 5
7
Figure 5.41
9
1
3
4 8
6 7
10
2
Figure 5.42
1
9 11 8
6 7
1. tegmentum 2. yolk 3. myotome
4. notochord 5. liver
10
2
6. gill arches 7. optic cup
8. forebrain ventricle 9. median fin fold
10. intestine 11. yolk extension
48 hours
47
Figure 5.43
1
2 3 7
5 6
4
Figure 5.44
4 8
1
3
7
2
5 6
9
Figure 5.45
4
1
8
3
2
7 6
1. tegmentum 2. median fin fold
9
10
3. yolk extension 4. yolk
5. gill arches 6. optic cup
7. forebrain ventricle 8. optic chiasm
9. lens 10. hypothalamus
48
ATLAS OF ZEBRAFISH DEVELOPMENT
Figure 5.46
3
1
2
4
5
6
10 9
87
Figure 5.47
3
1
2 4
10 9
8 7
5
6
Figure 5.48
3 1 2
4
6
10 9
1. tegmentum 2. optic chiasm
8 7
3. yolk 4. yolk extension
5. median fin fold 6. gill arches
7. hypothalamus 8. lens
9. optic cup 10. forebrain ventricle
48 hours
49
Figure 5.49
5 23
1
4 6
7
10 8
9
Figure 5.50
5 2
4
10 8
9
Figure 5.51
5
2
4
10 9
1. tegmentum 2. optic chiasm
3. lens 4. heart
5. yolk 6. yolk extension
7. gill arches 8. hypothalamus
9. optic cup 10. forebrain ventricle
50
ATLAS OF ZEBRAFISH DEVELOPMENT
Figure 5.52
4
2
3
1
6 5
Figure 5.53
4
7
3
1 5
Figure 5.54
4
3
1
1. olfactory bulb 2. optic chiasm
3. heart 4. yolk
5. optic cup
6. forebrain ventricle
7. olfactory pit
48 hours
51
Transverse Sections Figure 5.55
Figure 5.56
4 3 2
1
Figure 5.57
Figure 5.58
5
4
11
5
3
7 10
7
8 9
6
Figure 5.59
Figure 5.60 5
13
13
5
16 15
12 14
1. 2. 3. 4.
forebrain ventricle tectal ventricle tegmentum optic tectum
5. 6. 7. 8.
fourth ventricle olfactory bulb optic cup lens
9. 10. 11. 12.
anterior commissure hypothalamus cerebellum heart
13. 14. 15. 16.
medulla oblongata yolk gill arches otic vesicle
52
ATLAS OF ZEBRAFISH DEVELOPMENT
Figure 5.61
Figure 5.62 1
4
4
1
3 5
2
2
Figure 5.63
Figure 5.64 10
10
6 9
7
6
9
7
8
8
2
2
Figure 5.65
Figure 5.66
10 9
10
6
6
9 11
2
1. fourth ventricle 2. yolk 3. otic vesicle
2
4. medulla oblongata 5. gill arches 6. myotome
7. pectoral fin bud 8. liver 9. notochord
10. spinal cord 11. intestine
48 hours
53
Figure 5.67
Figure 5.68
4
4
1
3
2
2
Figure 5.69
Figure 5.70
4
4
1
3
5
Figure 5.71
Figure 5.72 6
6 4 3
4
1
3
1
5
5
2. yolk
1
3
2
1. myotome
1
3
3. notochord
4. spinal cord
5. yolk extension
6. median fin fold
54
ATLAS OF ZEBRAFISH DEVELOPMENT
Figure 5.73
Figure 5.74 1 5
1 5
2
4
3
3
Figure 5.75
Figure 5.76 1 5
1 5
2
4
2
4 3
3
Figure 5.77
Figure 5.78 1 5 4
1. median fin fold
2
4
2. myotome
1 5 4
2
3. yolk extension
2
4. notochord
5. spinal cord