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4830726 Separation of large DNA molecules in alternating asymmetric electric fields
PATENT
430
4828999 BACTERIOPHAGE PREVENTION AND CONTROL OF HARMFUL PLANT BACTERIA Le Roy Jackson For preventing or controlling bacterial harm to plants, as by disease or ice nucleation, a bateriophage composition of matter containing one or more viral h mutants specific to amutant of the bacteria concerned is produced and applied to seed, soil or soil supplements, plants. or plant materials that have been exposed to or are contaiminated with or infected by bacerial disease, or to growing plants subject to ice nucleation or other bacterial harm. The invention is concerned with the composition and with the method of producing and using same.
4829098 IMMOBILIZED BIOMOLECULES AND METHOD OF MAKING SAME Allan S Hoffman, Liang C Dong assigned Washington Research Foundation
to
A method of biomolecule immobilization is described in which the biomolecule itself or, alternatively, a monomer-conjugated btomolecule, is grafted with free monomer onto a hydrophilic, solid-phase, polymeric substrate which has been pre-irradiated with ionizing radiation. The pre-irradiation step is carried out, preferably at -78 degrees C. in air, while the grafting step is carried out at 0 degrees C. in a substantially oxygen-free atmosphere. The technique is applicable to immobilization of a wide variety of biomolecules, such as enzymes, catalysts, hormones, lectins, drugs, vitamins, antibodies, antigens, nucleic acids, DNA and RNA segments, pesticides, dyes and fertilizers. The products may be used for therapeutic or diagnostic applications or bioseparations.
4830726 SEPARATION OF LARGE DNA MOLECULES IN ALTERNATING ASYMMETRIC ELECTRIC FIELDS Thomas D Stamato, Nicholas to The Wistar Institute
Denko assigned
ABSTRACTS
A method of separating DNA molecules by gel electrophoresis is disclosed which employs alternate application of high and low strength electric fields in opposite directions to a gel matrix containing DNA. The high strength field is maintained for a shorter interval than the low strength field. and the ratio of the product of the low strength field and its pulse interval to the product of the high strength field and its pulse interval is greater than 4: I and sufficient to produce a net migration of the DNA molecules in the direction of the low strength field.
4830962 RECOMBINANT DIPHTHERIA TOXIN FRAGMENTS David H Gelfand, Lawrence I Greenfield, Frances C Lawyer assigned to Cetus Corpora tion Recombinant vectors which are effective in expressing DNA sequences encoding specific fragments of diphtheria toxins at high levels in recombinant host cells are disclosed. Both a fragment consisting of the enzymatically active A chain of diphtheria toxin and a fragment consisting of both the A portion and a B portion partial sequence are constructed by use of this recombinant vector.
4830965 TRANSPOSABLE LINKERS TO TRANSFER GENETIC INFORMATION Saran A Narang, John Goodchild, Ahmad 1 Bukhari, Nepean, MA, Canada assigned to Canadian Patents and Development Ltd; Cold Spring Harbor Laborato Transposable linkers are DNA sequences recognized and employed by transposition proteins, including transposases, in the insertion of genetic information into the genetic architecture of an organism and in the movement of genetic information from one location to another in the genetic architecture of an organism. Such linkers, comprising the extreme ends of transposons, often referred to as left and right attachment sites, have been isolated from the intervening material in transposons to produce a basic building block comprising simply the extreme ends of the transposons fused together. Using restriction endonuclease recognition sites
430
4828999 BACTERIOPHAGE PREVENTION AND CONTROL OF HARMFUL PLANT BACTERIA Le Roy Jackson For preventing or controlling bacterial harm to plants, as by disease or ice nucleation, a bateriophage composition of matter containing one or more viral h mutants specific to amutant of the bacteria concerned is produced and applied to seed, soil or soil supplements, plants. or plant materials that have been exposed to or are contaiminated with or infected by bacerial disease, or to growing plants subject to ice nucleation or other bacterial harm. The invention is concerned with the composition and with the method of producing and using same.
4829098 IMMOBILIZED BIOMOLECULES AND METHOD OF MAKING SAME Allan S Hoffman, Liang C Dong assigned Washington Research Foundation
to
A method of biomolecule immobilization is described in which the biomolecule itself or, alternatively, a monomer-conjugated btomolecule, is grafted with free monomer onto a hydrophilic, solid-phase, polymeric substrate which has been pre-irradiated with ionizing radiation. The pre-irradiation step is carried out, preferably at -78 degrees C. in air, while the grafting step is carried out at 0 degrees C. in a substantially oxygen-free atmosphere. The technique is applicable to immobilization of a wide variety of biomolecules, such as enzymes, catalysts, hormones, lectins, drugs, vitamins, antibodies, antigens, nucleic acids, DNA and RNA segments, pesticides, dyes and fertilizers. The products may be used for therapeutic or diagnostic applications or bioseparations.
4830726 SEPARATION OF LARGE DNA MOLECULES IN ALTERNATING ASYMMETRIC ELECTRIC FIELDS Thomas D Stamato, Nicholas to The Wistar Institute
Denko assigned
ABSTRACTS
A method of separating DNA molecules by gel electrophoresis is disclosed which employs alternate application of high and low strength electric fields in opposite directions to a gel matrix containing DNA. The high strength field is maintained for a shorter interval than the low strength field. and the ratio of the product of the low strength field and its pulse interval to the product of the high strength field and its pulse interval is greater than 4: I and sufficient to produce a net migration of the DNA molecules in the direction of the low strength field.
4830962 RECOMBINANT DIPHTHERIA TOXIN FRAGMENTS David H Gelfand, Lawrence I Greenfield, Frances C Lawyer assigned to Cetus Corpora tion Recombinant vectors which are effective in expressing DNA sequences encoding specific fragments of diphtheria toxins at high levels in recombinant host cells are disclosed. Both a fragment consisting of the enzymatically active A chain of diphtheria toxin and a fragment consisting of both the A portion and a B portion partial sequence are constructed by use of this recombinant vector.
4830965 TRANSPOSABLE LINKERS TO TRANSFER GENETIC INFORMATION Saran A Narang, John Goodchild, Ahmad 1 Bukhari, Nepean, MA, Canada assigned to Canadian Patents and Development Ltd; Cold Spring Harbor Laborato Transposable linkers are DNA sequences recognized and employed by transposition proteins, including transposases, in the insertion of genetic information into the genetic architecture of an organism and in the movement of genetic information from one location to another in the genetic architecture of an organism. Such linkers, comprising the extreme ends of transposons, often referred to as left and right attachment sites, have been isolated from the intervening material in transposons to produce a basic building block comprising simply the extreme ends of the transposons fused together. Using restriction endonuclease recognition sites