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4840714 Electroblotting technique for transferring specimens from a polyacrylamide electrophoresis or like gel onto a membrane
PATENT ABSTRACTS 4839291 DISPOSABLE BIOLOGICAL INDICATOR TEST PACK FOR MONITORING STEAM AND ETHYLENE OXIDE STERILIZATION CYCLES Jon D Welsh, Denis G Dyke assigned to American Sterilizer Company A disposable test pack for monitoring the el'ficacy of steam or ethylene oxide sterilization cycles includes a fiber board housing for a bioligical indicator. The housing includes an outer tube and a shorter inner tube. The outer tubehas upper and lower portions which define a seam or gap at their adjoining open ends. The inner tube extends past the seam and telescopes into the upper and lower portions. The seam and the close tolerance between the inner tube and the upper and lower portions of the outer tube provide a tortuous path for the entry of sterilant into the interior of the housing. The tortuous path has a moisture absorbent surface and is dimensioned to promote intimate contact between the sterilant and the absorbent surface. The exterior of the housing can be lined with foil to prevent sterilant from permeating the housing, thereby preventing entry of the steam by any path other than through the seam. A hole covered by an optionally removable tab is provided at the end of the upper portion of the outer tube for use in monitoring ethylene oxide sterilization cycles:
4839292 CELL CULTURE FLASK UTILIZING A MEMBRANE BARRIER Joseph G Cremonese A cell culture flask includes a hollow housing and a gas permeable membrane disposed within the housing which divides the housing into a first chamber and a second chamber. A retaining ring secures the gas permeable membrane within the housing and forms a liquid-tight seal between the first chamber and the second chamber. A gas inlet in the housing communicates with the first chamber and supplies gas thereto. A media inlet in the housing communicates with the second chamber and provides cell growth media thereto,
399
4839419 METHOD FOR IMMOBILIZING DISSOLVED PROTEINS Dieter Kraemer, Hermann Plainer, Bruno Sproessier, Helmut Uhlig, Reiner Schnee, Mainz, Federal Republic Of Germany assigned to Rohm GmbH Methods for adsorbing a protein, for example an enzyme, onto an insoluble, solid, macroporous, small-particle support by washing said support with an aqueous solution of the protein conraining an electrolyte in an ionic strength of at least 0.15 mole/liter and cr6sslinking said protcin, before, during, or after such adsorption, with a coupling component present in aqueous electrolyte-containing solution. 4839439 PREPARATION OF POLYMER BASED ON POLYVINYLENE C A R B O N A T E AND/OR POLYHYDROXYMETHYLENE Otto Mauz, Liederbach, Federal Republic Of Germany assigned to Hoechst Aktiengesellcshaft The invention relates to modified polymers based on polyvinylene carbonate and/or polyhydroxymethylene, the modification being effected by particular alkoxylated compounds incorporated into the polymer. These polymers, for the preparation of which, according to the invention, particular dispersion stabilizers are used, are suitable, inter alia, as carriers for biologically active substances or for affinity chromatography.
4840714 ELECTROBLOTTING TECHNIQUE FOR TRANSFERRING SPECIMENS FROM A POLYACRYLAMIDE ELECTROPHORESIS OR LIKE GEL ONTO A MEMBRANE William J Littlehales assigned to American Bionetics Inc An apparatus especially suitable for transferring a particular specimen from a polyacrylamide
40o
PATENT ABSTRACTS
electrophoresis or like gel onto a support sheet by means of electroblotting is disclosed herein along with its method of operation. This apparatus utilizes a pair of plate-shaped electrode assemblies, each of which in the disclosed embodiment is a multilayered assembly including an electrode layer of electrically conductive material and a layer of thermally conductive material which serves as a heat sink. These electrode assemblies are adjustably supported in spaced-apart but confronting relationship to one another with the gel and support sheet sandwiched therebetween. At the same time, the electrode layers are connected to a source of power for producing an electric field across the gel and support sheet in order to transfer the specimen from the gel to the sheet by means ofelectroblotting. In the specific embodiment disclosed, the spacing between the electrode assemblies can be adjustably decreased at a number of different points in order to ensure that they remain parallel to one another in their adjusted positions whereby to squeeze the gel and support sheet uniformly along the extend of the gel. 4840730 CHROMATOGRAPHY SYSTEM USING HORIZONTAL FLOW COLUMNS Vini Saxena assigned to Sepragen Corporation A system for chromatography using horizontal (radial) flow columns is disclosed. The system provides rapid separation of complex biological or organic mixtures at low operating pressures, The system may be used in a variety of applications, such as in the analysis of the constituents of a mixture, or in large pilot or plant scale operations where the isolation of one or more purified components of a mixture or fluid is required for further testing or for commercial purposes. The system is designed to accommodate separation via a variety of routes including ionexchange, affinity, hydrophobic, chiral, hydroxylapatite, reverse phased as well as rapid desalting techniques. 4840756 RADIATION PROCESS FOR PREPARATION OF ELECTROPHORESlS GEL MATERIAL Richard C Ebersole, Robert P Foss assigned to E I Du Pont Nemours and Company
Describes a process for controlling the polymerization and cross-linked density of electrophoretic gel products useful for separation of bioorganic molecules, which process does not use initiators common to processes of the art. Electron beam polymerized gels afford the desired advantages of being ultra thin and having a high electrophoretic resolution with programmable porosity profiles.
4840784 USE OF PYRYLIUM AND THIAPYRYLIUM COMPOUNDS AS BIOL OGICAL S T AINS David S Frank, Robert Belly assigned to Eastman Kodak Company Disclosed herein is an improvement in a method for distinguishing cells in a biological sample by staining with a dye, wherein the improvement comprises employing as the dye a compound of the formula See Patent for Chemical Structure wherein G is O or S; R1, R3, and R5 are independently selected from the group consisting of hydrogen, alkyl, aryl, aralkyl, amino, styryl, bis(diaryl)vinylene, and See Patent for Chemical Structure wherein R is hydrogen or alkyl; Z represents the elements necessary to complete a basic heterocyclic ring system of thetype used in cyanine dyes; n is 0 or 1; R2 is hydrogen or, taken together with either R 1 or R3, represents the elements needed to complete an aromatic or a carbocyclic ring system; R4 is hydrogen or, taken together with either R3 or R5, represents the elements needed to complete an aromatic or a carbocyclic ring system; and X- is an anion.
4840892 POLYNUCLEOTIDE HYBRIDIZATION
PROBES
Craig W Adams, Jeffry J Leary, Martin Rosenberg assigned to Smithkline Beckman Corporation Polynucleotide hybridization probes are labeled in non-probe regions but not in probe regions by selectively protecting probe regions prior to modification of the polynucleotide.
4839292 CELL CULTURE FLASK UTILIZING A MEMBRANE BARRIER Joseph G Cremonese A cell culture flask includes a hollow housing and a gas permeable membrane disposed within the housing which divides the housing into a first chamber and a second chamber. A retaining ring secures the gas permeable membrane within the housing and forms a liquid-tight seal between the first chamber and the second chamber. A gas inlet in the housing communicates with the first chamber and supplies gas thereto. A media inlet in the housing communicates with the second chamber and provides cell growth media thereto,
399
4839419 METHOD FOR IMMOBILIZING DISSOLVED PROTEINS Dieter Kraemer, Hermann Plainer, Bruno Sproessier, Helmut Uhlig, Reiner Schnee, Mainz, Federal Republic Of Germany assigned to Rohm GmbH Methods for adsorbing a protein, for example an enzyme, onto an insoluble, solid, macroporous, small-particle support by washing said support with an aqueous solution of the protein conraining an electrolyte in an ionic strength of at least 0.15 mole/liter and cr6sslinking said protcin, before, during, or after such adsorption, with a coupling component present in aqueous electrolyte-containing solution. 4839439 PREPARATION OF POLYMER BASED ON POLYVINYLENE C A R B O N A T E AND/OR POLYHYDROXYMETHYLENE Otto Mauz, Liederbach, Federal Republic Of Germany assigned to Hoechst Aktiengesellcshaft The invention relates to modified polymers based on polyvinylene carbonate and/or polyhydroxymethylene, the modification being effected by particular alkoxylated compounds incorporated into the polymer. These polymers, for the preparation of which, according to the invention, particular dispersion stabilizers are used, are suitable, inter alia, as carriers for biologically active substances or for affinity chromatography.
4840714 ELECTROBLOTTING TECHNIQUE FOR TRANSFERRING SPECIMENS FROM A POLYACRYLAMIDE ELECTROPHORESIS OR LIKE GEL ONTO A MEMBRANE William J Littlehales assigned to American Bionetics Inc An apparatus especially suitable for transferring a particular specimen from a polyacrylamide
40o
PATENT ABSTRACTS
electrophoresis or like gel onto a support sheet by means of electroblotting is disclosed herein along with its method of operation. This apparatus utilizes a pair of plate-shaped electrode assemblies, each of which in the disclosed embodiment is a multilayered assembly including an electrode layer of electrically conductive material and a layer of thermally conductive material which serves as a heat sink. These electrode assemblies are adjustably supported in spaced-apart but confronting relationship to one another with the gel and support sheet sandwiched therebetween. At the same time, the electrode layers are connected to a source of power for producing an electric field across the gel and support sheet in order to transfer the specimen from the gel to the sheet by means ofelectroblotting. In the specific embodiment disclosed, the spacing between the electrode assemblies can be adjustably decreased at a number of different points in order to ensure that they remain parallel to one another in their adjusted positions whereby to squeeze the gel and support sheet uniformly along the extend of the gel. 4840730 CHROMATOGRAPHY SYSTEM USING HORIZONTAL FLOW COLUMNS Vini Saxena assigned to Sepragen Corporation A system for chromatography using horizontal (radial) flow columns is disclosed. The system provides rapid separation of complex biological or organic mixtures at low operating pressures, The system may be used in a variety of applications, such as in the analysis of the constituents of a mixture, or in large pilot or plant scale operations where the isolation of one or more purified components of a mixture or fluid is required for further testing or for commercial purposes. The system is designed to accommodate separation via a variety of routes including ionexchange, affinity, hydrophobic, chiral, hydroxylapatite, reverse phased as well as rapid desalting techniques. 4840756 RADIATION PROCESS FOR PREPARATION OF ELECTROPHORESlS GEL MATERIAL Richard C Ebersole, Robert P Foss assigned to E I Du Pont Nemours and Company
Describes a process for controlling the polymerization and cross-linked density of electrophoretic gel products useful for separation of bioorganic molecules, which process does not use initiators common to processes of the art. Electron beam polymerized gels afford the desired advantages of being ultra thin and having a high electrophoretic resolution with programmable porosity profiles.
4840784 USE OF PYRYLIUM AND THIAPYRYLIUM COMPOUNDS AS BIOL OGICAL S T AINS David S Frank, Robert Belly assigned to Eastman Kodak Company Disclosed herein is an improvement in a method for distinguishing cells in a biological sample by staining with a dye, wherein the improvement comprises employing as the dye a compound of the formula See Patent for Chemical Structure wherein G is O or S; R1, R3, and R5 are independently selected from the group consisting of hydrogen, alkyl, aryl, aralkyl, amino, styryl, bis(diaryl)vinylene, and See Patent for Chemical Structure wherein R is hydrogen or alkyl; Z represents the elements necessary to complete a basic heterocyclic ring system of thetype used in cyanine dyes; n is 0 or 1; R2 is hydrogen or, taken together with either R 1 or R3, represents the elements needed to complete an aromatic or a carbocyclic ring system; R4 is hydrogen or, taken together with either R3 or R5, represents the elements needed to complete an aromatic or a carbocyclic ring system; and X- is an anion.
4840892 POLYNUCLEOTIDE HYBRIDIZATION
PROBES
Craig W Adams, Jeffry J Leary, Martin Rosenberg assigned to Smithkline Beckman Corporation Polynucleotide hybridization probes are labeled in non-probe regions but not in probe regions by selectively protecting probe regions prior to modification of the polynucleotide.