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4840730 Chromatography system using horizontal flow columns
40o
PATENT ABSTRACTS
electrophoresis or like gel onto a support sheet by means of electroblotting is disclosed herein along with its method of operation. This apparatus utilizes a pair of plate-shaped electrode assemblies, each of which in the disclosed embodiment is a multilayered assembly including an electrode layer of electrically conductive material and a layer of thermally conductive material which serves as a heat sink. These electrode assemblies are adjustably supported in spaced-apart but confronting relationship to one another with the gel and support sheet sandwiched therebetween. At the same time, the electrode layers are connected to a source of power for producing an electric field across the gel and support sheet in order to transfer the specimen from the gel to the sheet by means ofelectroblotting. In the specific embodiment disclosed, the spacing between the electrode assemblies can be adjustably decreased at a number of different points in order to ensure that they remain parallel to one another in their adjusted positions whereby to squeeze the gel and support sheet uniformly along the extend of the gel. 4840730 CHROMATOGRAPHY SYSTEM USING HORIZONTAL FLOW COLUMNS Vini Saxena assigned to Sepragen Corporation A system for chromatography using horizontal (radial) flow columns is disclosed. The system provides rapid separation of complex biological or organic mixtures at low operating pressures, The system may be used in a variety of applications, such as in the analysis of the constituents of a mixture, or in large pilot or plant scale operations where the isolation of one or more purified components of a mixture or fluid is required for further testing or for commercial purposes. The system is designed to accommodate separation via a variety of routes including ionexchange, affinity, hydrophobic, chiral, hydroxylapatite, reverse phased as well as rapid desalting techniques. 4840756 RADIATION PROCESS FOR PREPARATION OF ELECTROPHORESlS GEL MATERIAL Richard C Ebersole, Robert P Foss assigned to E I Du Pont Nemours and Company
Describes a process for controlling the polymerization and cross-linked density of electrophoretic gel products useful for separation of bioorganic molecules, which process does not use initiators common to processes of the art. Electron beam polymerized gels afford the desired advantages of being ultra thin and having a high electrophoretic resolution with programmable porosity profiles.
4840784 USE OF PYRYLIUM AND THIAPYRYLIUM COMPOUNDS AS BIOL OGICAL S T AINS David S Frank, Robert Belly assigned to Eastman Kodak Company Disclosed herein is an improvement in a method for distinguishing cells in a biological sample by staining with a dye, wherein the improvement comprises employing as the dye a compound of the formula See Patent for Chemical Structure wherein G is O or S; R1, R3, and R5 are independently selected from the group consisting of hydrogen, alkyl, aryl, aralkyl, amino, styryl, bis(diaryl)vinylene, and See Patent for Chemical Structure wherein R is hydrogen or alkyl; Z represents the elements necessary to complete a basic heterocyclic ring system of thetype used in cyanine dyes; n is 0 or 1; R2 is hydrogen or, taken together with either R 1 or R3, represents the elements needed to complete an aromatic or a carbocyclic ring system; R4 is hydrogen or, taken together with either R3 or R5, represents the elements needed to complete an aromatic or a carbocyclic ring system; and X- is an anion.
4840892 POLYNUCLEOTIDE HYBRIDIZATION
PROBES
Craig W Adams, Jeffry J Leary, Martin Rosenberg assigned to Smithkline Beckman Corporation Polynucleotide hybridization probes are labeled in non-probe regions but not in probe regions by selectively protecting probe regions prior to modification of the polynucleotide.
PATENT ABSTRACTS
electrophoresis or like gel onto a support sheet by means of electroblotting is disclosed herein along with its method of operation. This apparatus utilizes a pair of plate-shaped electrode assemblies, each of which in the disclosed embodiment is a multilayered assembly including an electrode layer of electrically conductive material and a layer of thermally conductive material which serves as a heat sink. These electrode assemblies are adjustably supported in spaced-apart but confronting relationship to one another with the gel and support sheet sandwiched therebetween. At the same time, the electrode layers are connected to a source of power for producing an electric field across the gel and support sheet in order to transfer the specimen from the gel to the sheet by means ofelectroblotting. In the specific embodiment disclosed, the spacing between the electrode assemblies can be adjustably decreased at a number of different points in order to ensure that they remain parallel to one another in their adjusted positions whereby to squeeze the gel and support sheet uniformly along the extend of the gel. 4840730 CHROMATOGRAPHY SYSTEM USING HORIZONTAL FLOW COLUMNS Vini Saxena assigned to Sepragen Corporation A system for chromatography using horizontal (radial) flow columns is disclosed. The system provides rapid separation of complex biological or organic mixtures at low operating pressures, The system may be used in a variety of applications, such as in the analysis of the constituents of a mixture, or in large pilot or plant scale operations where the isolation of one or more purified components of a mixture or fluid is required for further testing or for commercial purposes. The system is designed to accommodate separation via a variety of routes including ionexchange, affinity, hydrophobic, chiral, hydroxylapatite, reverse phased as well as rapid desalting techniques. 4840756 RADIATION PROCESS FOR PREPARATION OF ELECTROPHORESlS GEL MATERIAL Richard C Ebersole, Robert P Foss assigned to E I Du Pont Nemours and Company
Describes a process for controlling the polymerization and cross-linked density of electrophoretic gel products useful for separation of bioorganic molecules, which process does not use initiators common to processes of the art. Electron beam polymerized gels afford the desired advantages of being ultra thin and having a high electrophoretic resolution with programmable porosity profiles.
4840784 USE OF PYRYLIUM AND THIAPYRYLIUM COMPOUNDS AS BIOL OGICAL S T AINS David S Frank, Robert Belly assigned to Eastman Kodak Company Disclosed herein is an improvement in a method for distinguishing cells in a biological sample by staining with a dye, wherein the improvement comprises employing as the dye a compound of the formula See Patent for Chemical Structure wherein G is O or S; R1, R3, and R5 are independently selected from the group consisting of hydrogen, alkyl, aryl, aralkyl, amino, styryl, bis(diaryl)vinylene, and See Patent for Chemical Structure wherein R is hydrogen or alkyl; Z represents the elements necessary to complete a basic heterocyclic ring system of thetype used in cyanine dyes; n is 0 or 1; R2 is hydrogen or, taken together with either R 1 or R3, represents the elements needed to complete an aromatic or a carbocyclic ring system; R4 is hydrogen or, taken together with either R3 or R5, represents the elements needed to complete an aromatic or a carbocyclic ring system; and X- is an anion.
4840892 POLYNUCLEOTIDE HYBRIDIZATION
PROBES
Craig W Adams, Jeffry J Leary, Martin Rosenberg assigned to Smithkline Beckman Corporation Polynucleotide hybridization probes are labeled in non-probe regions but not in probe regions by selectively protecting probe regions prior to modification of the polynucleotide.