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4865984 Dynamic continuous flow enzyme reactor
PATENT ABSTRACTS
628
Methods and compositions for increased stability of enzymes in oxidant dry bleach. Enzyme stability is adversely affected by increased temperature, humidity, and the presence of strong oxidants, such as peracids. The instant invention provides enzyme stability in the presence of oxidant bleaches by coating or encapsulating the enzyme, while providing enzyme solubility suitable for use in bleach mixtures upon introduction to an aqueous medium. Particularly, alkali and neutral materials act as protection agents, which neutralize oxidant species before they contact and denature the enzyme. Other standard bleaching composition adjuncts such as builders, fillers, buffers, brighteners, fragrances, and the like may be included in the enzyme-containing oxidant bleach composition in addition to the discrete coated enzyme granules.
4863728 MONOCLONAL ANTI-ALPHAAMYLASE ANTIBODY WHICH NON-SPECIFICALLY INHIBITS THE ENZYME ACTIVITY OF HUMAN ALPHA-AMYLASE Martin Gerber, Kurt W Naujoks, Weilheim Unterhausen, Federal Republic Of Germany assigned to Boehringer Mannheim GmbH The present invention provides a monoclonal anti-alpha-amylase antibody which nonspecifically inhibits the enzyme activity of human alpha-amylase by more than 90~o. The present invention also provides a process for obtaining this monoclonal antibody and a medicament containing it.
4865972 ANTIBODY-BASED BIOASSAY FOR ENZYME-INDUCING CHEMICALS Kenneth W Hunter assigned to Westinghouse Electric Corp An antibody-based bioassay for enzymeinducing chemicals is disclosed. The percent induction of an enzyme or class of related enzymes in living cells by an enzyme-inducing chemical in a sample is determined before and after the addition to the sample of an antibody to the chem-
ical. The concentration of the chemical before and after the addition of the antibody is determined by comparison to the percent induction by known concentrations of the chemical. This permits the determination of the concentration of the chemical in the sample.
4865984 DYNAMIC CONTINUOUS FLOW ENZYME REACTOR Yale Nemerson, Vincen Turitto assigned to Mount Sinai School of Medicine of the City University of New York A dynamic continuous flow enzyme reactor and method for carrying out and analyzing phospholipid-dependent enzyme reactions are provided. The enzyme reactor is a tubular member preferably a capillary tube, coated on its inner surface with a planar phospholipid bilayer membrane, optionally and preferably containing an enzyme or enzyme cofactor. The reactor is connectable at one end to a means for delivering fluid flow reagents to the reactor and is connectable at a second end to a means for analyzing an effluent exiting from the reactor. Preferably the enzyme cofactor in the phospholipid membrane is purified tissue factor and the fluid reagents are inactive blood clotting factors which become activated upon interaction with the phospholipid membrane through a tissue factor-mediated reaction.
4866087 CARBOXYALKYL UREA COMPOUNDS AND DERIVATIVES THEREOF USEFUL AS ANGIOTENSIN CONVERTING ENZYME INHIBITORS AND AS ANTIHYPERTENSIVES William J Greenlee, David Hangauer, Arthur A Patchett assigned to Merck & Co Inc Carboxyalkyl urea compounds and derivatives thereof are disclosed which are useful as angiotensin converting enzyme (ACE) inhibitors and as antihypertensives. These compounds and derivatives are represented by the general formula: See Patent for Chemical Structure An illustrative specie falling within this general formula is: See Patent for Tabular Presentation
628
Methods and compositions for increased stability of enzymes in oxidant dry bleach. Enzyme stability is adversely affected by increased temperature, humidity, and the presence of strong oxidants, such as peracids. The instant invention provides enzyme stability in the presence of oxidant bleaches by coating or encapsulating the enzyme, while providing enzyme solubility suitable for use in bleach mixtures upon introduction to an aqueous medium. Particularly, alkali and neutral materials act as protection agents, which neutralize oxidant species before they contact and denature the enzyme. Other standard bleaching composition adjuncts such as builders, fillers, buffers, brighteners, fragrances, and the like may be included in the enzyme-containing oxidant bleach composition in addition to the discrete coated enzyme granules.
4863728 MONOCLONAL ANTI-ALPHAAMYLASE ANTIBODY WHICH NON-SPECIFICALLY INHIBITS THE ENZYME ACTIVITY OF HUMAN ALPHA-AMYLASE Martin Gerber, Kurt W Naujoks, Weilheim Unterhausen, Federal Republic Of Germany assigned to Boehringer Mannheim GmbH The present invention provides a monoclonal anti-alpha-amylase antibody which nonspecifically inhibits the enzyme activity of human alpha-amylase by more than 90~o. The present invention also provides a process for obtaining this monoclonal antibody and a medicament containing it.
4865972 ANTIBODY-BASED BIOASSAY FOR ENZYME-INDUCING CHEMICALS Kenneth W Hunter assigned to Westinghouse Electric Corp An antibody-based bioassay for enzymeinducing chemicals is disclosed. The percent induction of an enzyme or class of related enzymes in living cells by an enzyme-inducing chemical in a sample is determined before and after the addition to the sample of an antibody to the chem-
ical. The concentration of the chemical before and after the addition of the antibody is determined by comparison to the percent induction by known concentrations of the chemical. This permits the determination of the concentration of the chemical in the sample.
4865984 DYNAMIC CONTINUOUS FLOW ENZYME REACTOR Yale Nemerson, Vincen Turitto assigned to Mount Sinai School of Medicine of the City University of New York A dynamic continuous flow enzyme reactor and method for carrying out and analyzing phospholipid-dependent enzyme reactions are provided. The enzyme reactor is a tubular member preferably a capillary tube, coated on its inner surface with a planar phospholipid bilayer membrane, optionally and preferably containing an enzyme or enzyme cofactor. The reactor is connectable at one end to a means for delivering fluid flow reagents to the reactor and is connectable at a second end to a means for analyzing an effluent exiting from the reactor. Preferably the enzyme cofactor in the phospholipid membrane is purified tissue factor and the fluid reagents are inactive blood clotting factors which become activated upon interaction with the phospholipid membrane through a tissue factor-mediated reaction.
4866087 CARBOXYALKYL UREA COMPOUNDS AND DERIVATIVES THEREOF USEFUL AS ANGIOTENSIN CONVERTING ENZYME INHIBITORS AND AS ANTIHYPERTENSIVES William J Greenlee, David Hangauer, Arthur A Patchett assigned to Merck & Co Inc Carboxyalkyl urea compounds and derivatives thereof are disclosed which are useful as angiotensin converting enzyme (ACE) inhibitors and as antihypertensives. These compounds and derivatives are represented by the general formula: See Patent for Chemical Structure An illustrative specie falling within this general formula is: See Patent for Tabular Presentation