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4867884 Separation of cyclodextrins by affinity chromatography
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PATENT ABSTRACTS 4867855
METHOD AND APPARATUS FOR SEPARATING COMPLEX MIXTURES OF BIO-ORGANIC MATERIALS William G Burton assigned to Bionovus Inc A method and apparatus is described for separating complex mixtures of components, particularly bio-organic molecules, preferably on the basis of two independent physical characteristics. A sample initially is resolved in a first stage to yield a fluid stream containing the components of the sample longitudinally separated. The fluid stream then is aligned incident to the inlet face of a gel slab positioned in an electric field, having a field strength sufficient to force the separated and charged components from the flowing stream into the gel slab substantially simultaneously with exposure of said charged components to the electric field. Gel electrophoresis of the sample can then proceed in a known manner, 4867884 SEPARATION OF CYCLODEXTRINS BY AFFINITY CHROMATOGRAPHY Jacob A Rendleman assigned to The United States of America as represented by the Secretary of Agriculture Mixtures containing cyclodextrins are fractionated by affinity chromatography on matrices bearing hydrophobic ligands. The size and structure of the hydrophobic ligands can be altered to change the relative affinity of the ligands for different cyclodextrins and thus influence chromatographic fractionation,
strips away the HPLC solvent as it emerges from the end of the HPLC column and leaves a residue suitable for mass-spectral analysis. The interface includes a portable, stand-alone apparatus having a plural stage vacuum station, a continuous ribbon or belt, a drive train magnetically coupled to an external drive motor, a calibrated HPLC delivery system, a heated probe tip and means located adjacent the probe tip for direct ionization of the residue on the belt. The interface is also capable ofbeing readily adapted to fit any mass spectrometer. 4868103
ANALYTE DETECTION MEANS
OF ENERGY
BY TRANSFER
Jannis Stavrianopoulos, Elaza Rabbani, Samuel B Abrams, James G Wetmur assigned to Enzo Biochem Inc A method is disclosed to detect the presence of an analyte. The method involves forming a complex comprising the analyte and a binding entity. The binding entity comprises a first partner of an energy transfer system. The complex is then contacted with a reporting entity to form a unit. The reporting entity comprises a second partner of the energy transfer system. The first partner and the second partner are within Furster's radius of each other in the formed unit. The unit is irradiated with energy which can only be absorbed by one of said partners, namely, the energy donor, which then emits fluorescent energy. Some of this energy is absorbed by the other of said partners, namely, the energy acceptor, which also emits fluorescent energy. However, the fluorescent energy of the energy acceptor is of longer wavelength and in addition may be of substantially greater duration than the fluorescent energy of the energy donor. The detection of fluorescence at the longer wavelength or after a given time interval verifies the presence of the analyte.
4867947 4868104 INTERFACE FOR LIQUID CHROMATOGRAPH-MASS SPECTROMETER
HOMOGENEOUS ASSAY FOR SPECIFIC POLYNUCLEOTIDES
Brian D Andresen, Eric R Fought assigned to Sepragen Corporation
Nurit Kurn, Chander Bahl, Edwin F Ullman assigned to Syntex (U S A ) Inc
A moving belt interface for real-time, highperformance liquid chromatograph (HPLC)/mass spectrometer (MS) analysis which
A method for determining the presence of a polynucleotide analyte in a sample suspected of containing the analyte is disclosed. The method
PATENT ABSTRACTS 4867855
METHOD AND APPARATUS FOR SEPARATING COMPLEX MIXTURES OF BIO-ORGANIC MATERIALS William G Burton assigned to Bionovus Inc A method and apparatus is described for separating complex mixtures of components, particularly bio-organic molecules, preferably on the basis of two independent physical characteristics. A sample initially is resolved in a first stage to yield a fluid stream containing the components of the sample longitudinally separated. The fluid stream then is aligned incident to the inlet face of a gel slab positioned in an electric field, having a field strength sufficient to force the separated and charged components from the flowing stream into the gel slab substantially simultaneously with exposure of said charged components to the electric field. Gel electrophoresis of the sample can then proceed in a known manner, 4867884 SEPARATION OF CYCLODEXTRINS BY AFFINITY CHROMATOGRAPHY Jacob A Rendleman assigned to The United States of America as represented by the Secretary of Agriculture Mixtures containing cyclodextrins are fractionated by affinity chromatography on matrices bearing hydrophobic ligands. The size and structure of the hydrophobic ligands can be altered to change the relative affinity of the ligands for different cyclodextrins and thus influence chromatographic fractionation,
strips away the HPLC solvent as it emerges from the end of the HPLC column and leaves a residue suitable for mass-spectral analysis. The interface includes a portable, stand-alone apparatus having a plural stage vacuum station, a continuous ribbon or belt, a drive train magnetically coupled to an external drive motor, a calibrated HPLC delivery system, a heated probe tip and means located adjacent the probe tip for direct ionization of the residue on the belt. The interface is also capable ofbeing readily adapted to fit any mass spectrometer. 4868103
ANALYTE DETECTION MEANS
OF ENERGY
BY TRANSFER
Jannis Stavrianopoulos, Elaza Rabbani, Samuel B Abrams, James G Wetmur assigned to Enzo Biochem Inc A method is disclosed to detect the presence of an analyte. The method involves forming a complex comprising the analyte and a binding entity. The binding entity comprises a first partner of an energy transfer system. The complex is then contacted with a reporting entity to form a unit. The reporting entity comprises a second partner of the energy transfer system. The first partner and the second partner are within Furster's radius of each other in the formed unit. The unit is irradiated with energy which can only be absorbed by one of said partners, namely, the energy donor, which then emits fluorescent energy. Some of this energy is absorbed by the other of said partners, namely, the energy acceptor, which also emits fluorescent energy. However, the fluorescent energy of the energy acceptor is of longer wavelength and in addition may be of substantially greater duration than the fluorescent energy of the energy donor. The detection of fluorescence at the longer wavelength or after a given time interval verifies the presence of the analyte.
4867947 4868104 INTERFACE FOR LIQUID CHROMATOGRAPH-MASS SPECTROMETER
HOMOGENEOUS ASSAY FOR SPECIFIC POLYNUCLEOTIDES
Brian D Andresen, Eric R Fought assigned to Sepragen Corporation
Nurit Kurn, Chander Bahl, Edwin F Ullman assigned to Syntex (U S A ) Inc
A moving belt interface for real-time, highperformance liquid chromatograph (HPLC)/mass spectrometer (MS) analysis which
A method for determining the presence of a polynucleotide analyte in a sample suspected of containing the analyte is disclosed. The method