4870023 Recombinant baculovirus occlusion bodies in vaccines and biological insecticides

PATENT ABSTRACTS employing a hybridization process, which tomprises: (1) a step of transferring a portion of genetic clones cultured on a culture medium onto a filter to fix them thereunto; (2) a step of hybridizing the genes of said clones fixed onto said filter with radioactively labeled probes; (3) a step of obtaining two dimensional information on the location of the radioactively labeled substances on the filter which comprises placing said filter having been subjected to the hybridization and a stimulable phosphor sheet in layers for a given period of time to cause said stimulable phosphor sheet to absorb at least a portion of radiation energy emitted by the radioactively labeled substances on the filter, exciting said sheet with an electromagnetic wave to release the radiation energy stored in said sheet as stimulated emission, and detecting the stimulated emission to obtain a locational information on the radioactively labeled substances on the filter; and (4) a step recovering the clones on the culture medium according to the obtained locational information, 4865968 DNA SEQUENCING Leslie E Orgel, James W Patrick assigned to The Salk Institute for Biological Studies A first mixture is prepared that contains labeled chain fragments which each has a common end adjacent to a primary nucleotide and a termination at a position from the primary through an nth nucleotide, the first mixture containing nucleotide chain fragments of each length from termination at the primary through termination of the nth nucleotide. A second mixture is prepared that contains labeled chain fragments beginning at the common end and terminating at positions from the first through the nth nucleotide, the second mixture containing chain fragments of each length terminating wherever either a first or a second of the four nucleotides occurs. A third mixture is prepared that contains labeled chain fragments beginning at the cornmon end and terminating at a position from the first through the nth nucleotide, the third mixture containing chain fragments of each length terminating wherever the first or a third of the four nucleotide sequences occurs. The chains are electrophoresed with the first mixture as the center lane. This three-lane system provides a unique band pattern fur each of the four nucleotides and permits the sequence to be read merely by directly comparing each of the flanking lanes with the fully stepped center lane. This system has important advantages in reducing reading

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errors, particularly when read with computerassisted scanning apparatus.

4870023 RECOMBINANT

BACULOVIRUS

OCCLUSION BODIES IN VACCINES AND BIOLOGICAL INSECTICIDES Malcolm J Fraser, Elliot Rosen, Victoria A Ploplis assigned to American Biogenetic Sciences Inc The present invention is directed to recombinant haculoviruses which encode fusion polyhedrin proteins capable of forming occlusion bodies containing foreign peptides. The recombinant baculoviruses of the invention are formed by insertion into or replacement of regions of the polyhedrin gene that are not essential for occlusion body formation, with foreign DNA fragments by recombinant DNA techniques. The recombinant occlusion bodies produced in accordance with the present invention have uses in vaccine formulations, immunoassays, immobilized enzyme reactions, as biological insecticides, and as expression vectors. 4880512 PULSED LIGHT SELECTIVE PHOTOLYSIS PROCESS FOR TREATMENT OF BIOLOGICAL MEDIA AND PRODUCTS MADE THEREBY Paul Cornelius, Robin Hochstrasser, Neville Kallenbach, HarveyRubin, GeorgeJTodaroassigned to Kollmorgen Corporation A novel irradiation process and products made thereby. The process treats biological media such as blood fractions, genetically engineered protein products and vaccine preparations. The process photolyzes nucleic acids in preference to proteins in the media, e.g., it inactivates DNAor RNA-containing pathogens while leaving the proteins substantially intact or functional. In general, the process comprises irradiating the medium with pulsed light of wavelength and flux selected so that (1) the nucleic acids in their ground state absorb radiation and thereby rise to an excited state or states, (2) the nucleic acids in their excited states absorb radiation and thereby rise to higher energy states and undergo photo-