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4885151 Diagnostic aid for the scintigraphic visualization of malignant tumors comprising the substance obtained from enzymatic processing of murein using Hen's eggwhite lysozyme
PATENT ABSTRACTS
630
This invention relates to a process for screening microorganisms for the production of extracellular enzymes. Colonies growing on the surface of a solid medium capable of extracellular enzyme synthesis are identified by reacting the enzyme or enzyme product surrounding each cology with an analytically indicatable reagent which does not adversely effect the viability of said colonies.
in step (b). By using the process it is possible to stably and efficiently carry out conversion of AMP into ATP over a long period of time. Further, it is possible to carry out continuously and economically an enzymic reaction using ATP as an auxiliary factor with very good efficiency. Whereby it has become possible to practically operate the so-called bioreactor wherein synthetic reactions in the living body are carried out as industrial chemical reactions outside the living body.
4882274 METHOD FOR SOLUBILIZATION OF LOW-RANK COAL USING A CELL-FREE ENZYMATIC SYSTEM John Pyne, Dorothy Stewart, James Fredrickson, Martin S Cohen assigned to Electric Power Research Institute Inc A method is provided for isolating an extracellular product from white rot fungi. The extracellular product is useful for biosolubilizing lowrank coals to form water-soluble products.
4882276 PROCESS FOR PRODUCING PHYSIOLOGICALLY ACTIVE SUBSTANCE BY MULTIENZYME PROCESS Kazutomo Imahori, Hitoshi Kondo, Hiroshi Nakajima, Tatsuo lwasaki, Tokyo, Japan assigned to Imahori Kazutomo; Kenkyusho Rikagaku; Unitika L A process for converting AMP into ATP which comprises (a) using an enzyme which converts AMP into ADP and has been produced from microorganisms having an optimum growth temperature of 50 degrees C. to 85 degrees C. and an enzyme which converts ADP into ATP and has been produced from microorganisms having an optimum growth temperature of 50 degrees to 85 degrees C. is disclosed. In addition, there is disclosed a process for producing a physiologically active substance by a multienzyme process which comprises forming ATP from AMP by the step (a), (b) synthesizing a physiologically active substance with the resulting ATP, coverting AMP resulting from the reaction in step (b) into ATP by the reaction in step (a), and repeatedly utilizing the converted ATP for synthesis of the physiologically active substance
4883760 DEVICE FOR PERFORMING ENZYME IMMUNOASSAYS Donald R Heelies, Mississauga, Canada assigned to Adi Diagnostics Inc The device has a transparent capillary assay tube attached at its upper end to a lateral portion of the support structure of the device. The lower free end of the assay tube is engageable with an absorbent material upon manual downward deflection of the support structure. The assay tube is filled through a funnel shaped aperture in the lateral portion of the device, and the tube is drained by engaging the lower free end of the tube with the absorbent material. By attaching appropriate antibody, antigen or hapten to the inner surface of the tube, immunoassays may be performed in the tube.
4885151 DIAGNOSTIC AID FOR THE SCINTIGRAPHIC VISUALIZATION OF MALIGNANT TUMORS COMPRISING THE SUBSTANCE OBTAINED FROM ENZYMATIC PROCESSING OF MUREIN USING HEN'S EGGWHITE LYSOZYME Erich Oberhausen, Ludwig Kuhlmann, Gerhard Seibert, Axe Steinstrasser, Hans-Joachi Schroth, Karl-Heinz Bremer, Homburg/Saar, Federal Republic Of Germany assigned to Hoechst Aktiengesellschaft A diagnostic aid for the scintigraphic visualization of malignant tumors which contains a radionuclide which is bonded by means of a complex-forming agent to the substance obtained by the enzymatic processing of murein
PATENT ABSTRACTS with enzyme consisting essentially of hen's eggwhite lysozyme.
4885250 ENZYME IMMOBILIZATION AND BIOAFFINITY SEPARATIONS WITH PERFLUOROCARBON POLYMER-BASED SUPPORTS John William Eveleigh, Robert K Kobos assigned to E I Du Pont De Nemours and Company A bioaffinity separation method is provided along with a solid affinity support utilized in that method. Additionally, immobilized enzyme systems are provided for use as enzyme electrode systems. The support is based on an inert perfluorocarbon polymer carrier with ligands or binders attached to its surface. The ligand, binder or enzyme is preferably modified by attaching a perfluorocarbon anchor group, and the modified ligand, binder or enzyme is attached to the carrier through the anchor group. Methods for preparing such supports and their use in capturing target molecules from samples and in analytical applications are also provided.
4886672 PROCESS FOR THE LIQUEFACTION OF BEETS AND CHICORY ROOTS BY ENZYMATIC HYDROLYSIS AND LIQUID HYDROLYSATE OBTAINED Baynast de Septfontaines Regis J M P de, Francois E M E Brouard, Jean-Luc A G Baret, Yvon G A J Gicquiaux, Hans Olsen, Versailles, France assigned to Sucre Recherches et Developpement; Novo Industri A A process for the liquifaction of beets or chicory roots which includes washing and grinding the beets or chicory roots to provide a ground product; mixing the ground product with a mixture of enzymes that include SPS-ase, cellulase and cellobiase, as well as an acid so as to provide a pH of about 3 to 5.5; leaving the mixture for about I to 6 hours to accomplish a prehydrolysis of the ground product; grinding the prehydrolyzed ground product; allowing the prehydrolyzed ground product to hydrolyze for about 20 to ! 20 hours; and recovering the liquid hydrolyzed product.
63:
4886740 ENZYME-ELECTRODE WITH ORGANOSILANE MEMBRANE
SENSOR TREATED
Pankaj M Vadgama, Newcastle Upon Tyne, United Kingdom assigned to Imperial Chemical Industries PLC A membrane for an enzyme-electrode type sensor treated to increase the range oflinearity of the sensor response.
4886742 ENZYME IMMUNOASSAY FOR DETECTING HIV ANTIGENS IN HUMAN SERA Kenneth Kortright, David E Hofheinz, Meryl Forman, Song Lee, Paulette Smariga, Candie S Stoner assigned to Coulter Corporation A solid-phase immunoassay is provided for determination of HIV antigens in human physiological fluid. The immunoassay is characterized by the coating of a solid substrate with a unique monoclonal antibody which recognizes a common antigenic determinant of a group of HIV core antigens and no HIV envelope antigens of HIV. The test sample preferably also is subjected to a lysing reagent prior to the incubation for uniformly dispersing antigens which may be present in the test sample.
4888285 ENZYME IMMOBILIZATION ON A WATER-INSOLUBLE AMINO GROUP-CONTAINING CARRIER Yuusaku Nishimura, Masahiko Ishida, Ryoichi Haga, Hitachi, Japan assigned to Hitachi Ltd An immobilized enzyme having high activity and stability is obtained by immobilizing an enzyme on a water-insoluble amino groupcontaining carrier by use of a polyfunctional cross-linking agent such as glutaraldehyde in the presence of a phenolic carboxylic acid having one or more hydroxyl groups such as tannic acid. In addition to the phenolic carboxylic acid, a basic polysaccharide such as chitosan may also be present. The amino group-containing carrier may be aminated silica gel, aminated porous
630
This invention relates to a process for screening microorganisms for the production of extracellular enzymes. Colonies growing on the surface of a solid medium capable of extracellular enzyme synthesis are identified by reacting the enzyme or enzyme product surrounding each cology with an analytically indicatable reagent which does not adversely effect the viability of said colonies.
in step (b). By using the process it is possible to stably and efficiently carry out conversion of AMP into ATP over a long period of time. Further, it is possible to carry out continuously and economically an enzymic reaction using ATP as an auxiliary factor with very good efficiency. Whereby it has become possible to practically operate the so-called bioreactor wherein synthetic reactions in the living body are carried out as industrial chemical reactions outside the living body.
4882274 METHOD FOR SOLUBILIZATION OF LOW-RANK COAL USING A CELL-FREE ENZYMATIC SYSTEM John Pyne, Dorothy Stewart, James Fredrickson, Martin S Cohen assigned to Electric Power Research Institute Inc A method is provided for isolating an extracellular product from white rot fungi. The extracellular product is useful for biosolubilizing lowrank coals to form water-soluble products.
4882276 PROCESS FOR PRODUCING PHYSIOLOGICALLY ACTIVE SUBSTANCE BY MULTIENZYME PROCESS Kazutomo Imahori, Hitoshi Kondo, Hiroshi Nakajima, Tatsuo lwasaki, Tokyo, Japan assigned to Imahori Kazutomo; Kenkyusho Rikagaku; Unitika L A process for converting AMP into ATP which comprises (a) using an enzyme which converts AMP into ADP and has been produced from microorganisms having an optimum growth temperature of 50 degrees C. to 85 degrees C. and an enzyme which converts ADP into ATP and has been produced from microorganisms having an optimum growth temperature of 50 degrees to 85 degrees C. is disclosed. In addition, there is disclosed a process for producing a physiologically active substance by a multienzyme process which comprises forming ATP from AMP by the step (a), (b) synthesizing a physiologically active substance with the resulting ATP, coverting AMP resulting from the reaction in step (b) into ATP by the reaction in step (a), and repeatedly utilizing the converted ATP for synthesis of the physiologically active substance
4883760 DEVICE FOR PERFORMING ENZYME IMMUNOASSAYS Donald R Heelies, Mississauga, Canada assigned to Adi Diagnostics Inc The device has a transparent capillary assay tube attached at its upper end to a lateral portion of the support structure of the device. The lower free end of the assay tube is engageable with an absorbent material upon manual downward deflection of the support structure. The assay tube is filled through a funnel shaped aperture in the lateral portion of the device, and the tube is drained by engaging the lower free end of the tube with the absorbent material. By attaching appropriate antibody, antigen or hapten to the inner surface of the tube, immunoassays may be performed in the tube.
4885151 DIAGNOSTIC AID FOR THE SCINTIGRAPHIC VISUALIZATION OF MALIGNANT TUMORS COMPRISING THE SUBSTANCE OBTAINED FROM ENZYMATIC PROCESSING OF MUREIN USING HEN'S EGGWHITE LYSOZYME Erich Oberhausen, Ludwig Kuhlmann, Gerhard Seibert, Axe Steinstrasser, Hans-Joachi Schroth, Karl-Heinz Bremer, Homburg/Saar, Federal Republic Of Germany assigned to Hoechst Aktiengesellschaft A diagnostic aid for the scintigraphic visualization of malignant tumors which contains a radionuclide which is bonded by means of a complex-forming agent to the substance obtained by the enzymatic processing of murein
PATENT ABSTRACTS with enzyme consisting essentially of hen's eggwhite lysozyme.
4885250 ENZYME IMMOBILIZATION AND BIOAFFINITY SEPARATIONS WITH PERFLUOROCARBON POLYMER-BASED SUPPORTS John William Eveleigh, Robert K Kobos assigned to E I Du Pont De Nemours and Company A bioaffinity separation method is provided along with a solid affinity support utilized in that method. Additionally, immobilized enzyme systems are provided for use as enzyme electrode systems. The support is based on an inert perfluorocarbon polymer carrier with ligands or binders attached to its surface. The ligand, binder or enzyme is preferably modified by attaching a perfluorocarbon anchor group, and the modified ligand, binder or enzyme is attached to the carrier through the anchor group. Methods for preparing such supports and their use in capturing target molecules from samples and in analytical applications are also provided.
4886672 PROCESS FOR THE LIQUEFACTION OF BEETS AND CHICORY ROOTS BY ENZYMATIC HYDROLYSIS AND LIQUID HYDROLYSATE OBTAINED Baynast de Septfontaines Regis J M P de, Francois E M E Brouard, Jean-Luc A G Baret, Yvon G A J Gicquiaux, Hans Olsen, Versailles, France assigned to Sucre Recherches et Developpement; Novo Industri A A process for the liquifaction of beets or chicory roots which includes washing and grinding the beets or chicory roots to provide a ground product; mixing the ground product with a mixture of enzymes that include SPS-ase, cellulase and cellobiase, as well as an acid so as to provide a pH of about 3 to 5.5; leaving the mixture for about I to 6 hours to accomplish a prehydrolysis of the ground product; grinding the prehydrolyzed ground product; allowing the prehydrolyzed ground product to hydrolyze for about 20 to ! 20 hours; and recovering the liquid hydrolyzed product.
63:
4886740 ENZYME-ELECTRODE WITH ORGANOSILANE MEMBRANE
SENSOR TREATED
Pankaj M Vadgama, Newcastle Upon Tyne, United Kingdom assigned to Imperial Chemical Industries PLC A membrane for an enzyme-electrode type sensor treated to increase the range oflinearity of the sensor response.
4886742 ENZYME IMMUNOASSAY FOR DETECTING HIV ANTIGENS IN HUMAN SERA Kenneth Kortright, David E Hofheinz, Meryl Forman, Song Lee, Paulette Smariga, Candie S Stoner assigned to Coulter Corporation A solid-phase immunoassay is provided for determination of HIV antigens in human physiological fluid. The immunoassay is characterized by the coating of a solid substrate with a unique monoclonal antibody which recognizes a common antigenic determinant of a group of HIV core antigens and no HIV envelope antigens of HIV. The test sample preferably also is subjected to a lysing reagent prior to the incubation for uniformly dispersing antigens which may be present in the test sample.
4888285 ENZYME IMMOBILIZATION ON A WATER-INSOLUBLE AMINO GROUP-CONTAINING CARRIER Yuusaku Nishimura, Masahiko Ishida, Ryoichi Haga, Hitachi, Japan assigned to Hitachi Ltd An immobilized enzyme having high activity and stability is obtained by immobilizing an enzyme on a water-insoluble amino groupcontaining carrier by use of a polyfunctional cross-linking agent such as glutaraldehyde in the presence of a phenolic carboxylic acid having one or more hydroxyl groups such as tannic acid. In addition to the phenolic carboxylic acid, a basic polysaccharide such as chitosan may also be present. The amino group-containing carrier may be aminated silica gel, aminated porous