4894338 Yeast strains producing cellulolytic enzymes and methods and means for constructing them

PATENT ABSTRACTS Constructions and methods for mutagenesis of nucleic acids involve chemical mutagenesis of a cassette comprising a structural gene linked to a non-functional restorable fragment of a marker gene. Mutants are detected by screening for the presence of the reconstituted marker among the ligation products of the cassette to a vector containing the non-functional restorable remainder of the marker gene. Xylose isomerase mutants, characterized by a change from glu (GAG) to lys (AAG) at amino acid position 262 in the xylA protein of E. coli were obtained by partial marker cassette mutagenesis. These mutants enzymes exhibited twice the rate of isomerization of glucose to fructose exhibited by the wild type.

4894332 DNA SEQUENCES CODING FOR MYCOPLASMA HYPOPNEUMONIAE SURFACE ANTIGENS, METHODS OF USE TO MAKE THE CORRESPONDING PROTEINS

587

48~3~ YEAST STRAINS PRODUCING CELLULOLYTIC ENZYMES AND METHODS AND MEANS FOR CONSTRUCTING THEM Jonathan Knowles, PenttilaMerja, Tuula Teeri, Helena Nevalainen, Irma Salovuori, Paiv Lehtovaara-Helenius, Helsinki, Finland assigned to Oy Alko AB The full length DNA sequence encoding the mature cellobiohydrolase II(CHBII), as well as the DNA sequence encoding the CBHII signal sequence, have been isolated and cloned from the fungus Trichoderma reesei. Recombinant vectors comprising these sequences, and yeast hosts transformed with such vectors are disclosed. Also disclosed are methods for constructing the vectors and hosts according to the invention, as well as methods for expressing the disclosed sequences. CBHII is involved in hydrolysis of cellulose.

Heinz E Schaller, Mo-Quen Klinkert, Heidelberg, Federal Republic Of Germany assigned to Biogen Inc Surface antigens of swine mycoplasma, such antigens prepared by recombinant D N A methods, a swine mycoplasma vaccine, based on such antigens, methods of treating swine to prevent enzootic pneumonia using that vaccine, and diagnostic tests based on these antigens or antibodies raised against them for detecting the presence of swine mycoplasma infections in swine herds, and DNA sequences that code for such antigens are disclosed.

4894333 BOVINE

INTERLEUKIN-I£60

Douglas P Cerretti, Charles R Maliszewski, Michael A Schoenborn assigned to Immunex Corporation Cloning and expression of DNA segments encoding bovine IL- alpha, and processes for producing purified bovine IL-1 alpha as a product of recombinant cell culture, are disclosed.

4894M1 PRODUC~ON OF CYANIDE HYDRATASE Kenneth Richardson, Middlesbrough, United Kingdom assigned to Imperial Chemical Industries PLC A process tbr the production of the enzyme cyanide hydratase which comprises aerobically cultivating cells of the fungal strain Fusarium lateritium Nees CMI 300533 and variants and mutants derives therefrom in an aqueous culture containing appropriate nutrients. A method of treating a cyanide-containing material to degrade the cyanide therein using cyanide hydratase produced by the process of the invention and a process for the production of a nitrilase enzyme are also claimed. Fusarium lateritium Nees CMI 300533 is deposited at The Commonwealth Mycological Institute, Kew, Richmond, Surrey, England under the terms of the Budapest Treaty.