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4894339 Immobilized enzyme membrane for a semiconductor sensor
PATENT ABSTRACTS
4894339 IMMOBILIZED ENZYME MEMBRANE FOR A SEMICONDUCTOR SENSOR Yoshio Hanazato, Satoru Shiono, Mamiko Nakako, Satoshi Yamada, Hon, Japan assigned to Seitaikinouriyou Kagakuhin Sinseizogijutsu Kenkyu Kumiai A membrane containing an immobilized enzyme for a semiconductor sensor is prepared containing a water soluble photosensitive resin including a high molecular weight polyvinyl pyrrolidone crosslinked to 2, 5-bis (4'-azide-2'sulfobenzal) cyclopentanone sodium salt, and an enzyme. Glutaraldehyde and bovine serum albumin, polyamino acid or polyamino amino acid copolymer may also be present to provide chemical crosslinking. The enzyme may be glucose oxidase, urease or lipase. The membrane can be directly formed on ion-sensitive protions of a pH-ion sensitive field effect transistor to form a semiconductor sensor by coating an aqueous solution of the resin and enzyme on the ion-sensitive portion, drying and irradiating with light such as ultraviolet light to provide photo crosslinking.
4894342 BIOREACTOR
SYSTEM
Perry W Guinn, Gary N Mills assigned to C D Vledical Inc An improved bioreactor system for culturing :ell products is disclosed in which novel system ;omponents are employed to reduce cost and in:rease reliability. The system includes a simple n-line heater for heating the nutrient fluid, Lhereby obviating the need for an expensive in:ubator. Gas bubbles formed during the heating 3f the nutrient fluid are removed by a novel gas Lrap that requires no operator intervention. A humidifier humidifies an aerating gas before it is passed through an oxygenator, thereby preventing water within the nutrient fluid from ~vaporating, with a consequent increase in nutrient fluid concentration. The operation of the humidifier is efficiently combined with that of a nutrient fluid heat exchanger so that a pump is not required to circulate the heat exchanger water bath. The system further includes a valve and pump arrangement whereby fresh nutrient fluid can periodically be introduced and spent
nutrient fluid periodically drained without operator intervention. Observation of the color of the nutrient fluid from a concealed location within the bioreactor enclosure is also made possible.
4894347 ERYTHROCYTE AGGLUTINATION ASSAY Carmel J Hillyard, Dennis Rylatt, Bruce E Kemp, Peter G Bundesen, Brisbane, Australia assigned to Agen Limited In an erythrocyte agglutination assay, the agglutination reagent comprises at least one erythrocyte binding molecule coupled to at least one specific analyte binding molecule wherein the erythrocyte binding molecule does not cause agglutination when incubated with erythrocytes in the absence of analyte. Preferably, the erythrocytes are endogenous to the blood sample to be tested, that is, a whole blood sample is assayed. Mixtures of conjugates and conjugates of analyte analogues with erythrocyte binding molecules may also be used as agglutination reagents. The reagents and their use in direct or indirect assays is disclosed.
4894348 FLUORESCEIN-CONJUGATED PROTEINS WITH ENHANCED FLUORESCENCE Robert Ronald, Phuc Nguyen, Gerald L Rowley A method for conducting assays for an analyte, usually in a biological sample, utilizes reagents which contain biological ligands linked to fluorescein or a derivative of fluorescein through a linkage to the fluorescein nucleus selected from acetamido and thioacetamido. In these reagents, the fluorescence efficiency is improved over that obtained in the commonly used FITC-labeled reagents.
4894440 METHOD OF ISOLATING MEGAKARYOCYTE STIMULATORY FACTOR Robert D Rosenberg assigned to Massachusetts Institute of Technology
4894339 IMMOBILIZED ENZYME MEMBRANE FOR A SEMICONDUCTOR SENSOR Yoshio Hanazato, Satoru Shiono, Mamiko Nakako, Satoshi Yamada, Hon, Japan assigned to Seitaikinouriyou Kagakuhin Sinseizogijutsu Kenkyu Kumiai A membrane containing an immobilized enzyme for a semiconductor sensor is prepared containing a water soluble photosensitive resin including a high molecular weight polyvinyl pyrrolidone crosslinked to 2, 5-bis (4'-azide-2'sulfobenzal) cyclopentanone sodium salt, and an enzyme. Glutaraldehyde and bovine serum albumin, polyamino acid or polyamino amino acid copolymer may also be present to provide chemical crosslinking. The enzyme may be glucose oxidase, urease or lipase. The membrane can be directly formed on ion-sensitive protions of a pH-ion sensitive field effect transistor to form a semiconductor sensor by coating an aqueous solution of the resin and enzyme on the ion-sensitive portion, drying and irradiating with light such as ultraviolet light to provide photo crosslinking.
4894342 BIOREACTOR
SYSTEM
Perry W Guinn, Gary N Mills assigned to C D Vledical Inc An improved bioreactor system for culturing :ell products is disclosed in which novel system ;omponents are employed to reduce cost and in:rease reliability. The system includes a simple n-line heater for heating the nutrient fluid, Lhereby obviating the need for an expensive in:ubator. Gas bubbles formed during the heating 3f the nutrient fluid are removed by a novel gas Lrap that requires no operator intervention. A humidifier humidifies an aerating gas before it is passed through an oxygenator, thereby preventing water within the nutrient fluid from ~vaporating, with a consequent increase in nutrient fluid concentration. The operation of the humidifier is efficiently combined with that of a nutrient fluid heat exchanger so that a pump is not required to circulate the heat exchanger water bath. The system further includes a valve and pump arrangement whereby fresh nutrient fluid can periodically be introduced and spent
nutrient fluid periodically drained without operator intervention. Observation of the color of the nutrient fluid from a concealed location within the bioreactor enclosure is also made possible.
4894347 ERYTHROCYTE AGGLUTINATION ASSAY Carmel J Hillyard, Dennis Rylatt, Bruce E Kemp, Peter G Bundesen, Brisbane, Australia assigned to Agen Limited In an erythrocyte agglutination assay, the agglutination reagent comprises at least one erythrocyte binding molecule coupled to at least one specific analyte binding molecule wherein the erythrocyte binding molecule does not cause agglutination when incubated with erythrocytes in the absence of analyte. Preferably, the erythrocytes are endogenous to the blood sample to be tested, that is, a whole blood sample is assayed. Mixtures of conjugates and conjugates of analyte analogues with erythrocyte binding molecules may also be used as agglutination reagents. The reagents and their use in direct or indirect assays is disclosed.
4894348 FLUORESCEIN-CONJUGATED PROTEINS WITH ENHANCED FLUORESCENCE Robert Ronald, Phuc Nguyen, Gerald L Rowley A method for conducting assays for an analyte, usually in a biological sample, utilizes reagents which contain biological ligands linked to fluorescein or a derivative of fluorescein through a linkage to the fluorescein nucleus selected from acetamido and thioacetamido. In these reagents, the fluorescence efficiency is improved over that obtained in the commonly used FITC-labeled reagents.
4894440 METHOD OF ISOLATING MEGAKARYOCYTE STIMULATORY FACTOR Robert D Rosenberg assigned to Massachusetts Institute of Technology