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4909941 High performance liquid chromatography mobile phase
PATENT ABSTRACTS
596
are provided. A hybridization probe constructed to contain thionucleotides and a detectable label is hybridized to a nucleotide sequence of interest in the absence of nonpolar solvents, reducing agents and volume exclusion agents. The thionucleotides serve to increase the mass of bound probe molecules, thereby amplifying the signal available to detect the presence of the nucleic acid sequence of interest. Certain cations interact specifically with the thiol moiety of phosphorothioate groups of nucelic acid molecules in a manner that can be exploited to amplify probe binding or convey a detectable label. Thus, the mass of bound probe molecules can be further increased by the presence of specified multivalent cations from the transition metal group, such as Cu2 + or Fe3 + , during the annealing step.
4908773 COMPUTER DESIGNED STABILIZED PROTEINS AND METHOD FOR PRODUCING SAME Michael Pantoliano, Robert C Ladner assigned to Genex Corporation Proteins, such as enzymes having enhanced stability are designed through the use of a computer method. The method identifies amino acid residues of a protein which may be replaced with a cysteine residue in order to promote the formation of a protein-stabilizing disulfide bond. The computer-designed, stabilized proteins can be produced using recombinant DNA technology.
4908312 4909941 PROCESS FOR PRODUCING PHENYLANANINE Akio Ozaki, Ryoichi Katsumata, Tetsuo Oka, Machida, Japan assigned to Kyowa Hakko Kogyo Co Ltd The invention relates to a bacterial process for producing phenylalanine. The process utilizes a Corynebacterium or Brevibacterium host which is transformed with a recombinant DNA. The recombinant D N A harbors a D N A containing a gene which encodes chorismate mutase or prephenate dehydratase. The transformed microorganism is then cultured in order to accumulate phenylalanine in the culture medium and the phenylalanine is recovered therefrom.
4908440 2',3'-DIDEOXY-2' FLUOROARABINOPYRIMIDINE NUCLEOSIDES Roman Z Sterzycki, Muzammil M Mansuri, John C Martin assigned to Bristol Myers Company There are disclosed novel 2',3'-dideoxy-2' fluoronucleosides and 2'-3'-dideoxy-2' 3"didehydro-2' fluoronucleosides and processes for their preparation. The compounds so produced exhibit therapeutically useful antiviral and, more particularly, anti-HIV effects.
HIGH PERFORMANCE CHROMATOGRAPHY PHASE
LIQUID MOBILE
Dick J Poll, David R K Harding, William S Hancock, Palmerston North, New Zealand There is described a mobile phase for use in the purification of proteins using reversed phase HPLC on a preparative scale. The mobile phase is an aqueous solution containing 0.005 to IM of a physiologicially acceptable acid of the general formula YCOOH where Y is a polar electron withdrawing group capable of hydrogen bonding silanol groups. The solution also contains up to 95% by volume of a physilogically acceptable organic solvent. A preparative method of purifying proteins such as HSA or BSA and recombinant deoxyribonucleic proteins using reversed phased PHLC is also described.
4910016 NOVEL BACILLUS THURINGIENSIS ISOLATE Frank H Gaertner, George G Soares, Jewel Payne assigned to Mycogen Corporation The subject invention concerns a novel and useful insecticide with activity against insect pests of the order Coleoptera and the order Lepidoptera. Pests in the order Coleoptera do heavy damage
596
are provided. A hybridization probe constructed to contain thionucleotides and a detectable label is hybridized to a nucleotide sequence of interest in the absence of nonpolar solvents, reducing agents and volume exclusion agents. The thionucleotides serve to increase the mass of bound probe molecules, thereby amplifying the signal available to detect the presence of the nucleic acid sequence of interest. Certain cations interact specifically with the thiol moiety of phosphorothioate groups of nucelic acid molecules in a manner that can be exploited to amplify probe binding or convey a detectable label. Thus, the mass of bound probe molecules can be further increased by the presence of specified multivalent cations from the transition metal group, such as Cu2 + or Fe3 + , during the annealing step.
4908773 COMPUTER DESIGNED STABILIZED PROTEINS AND METHOD FOR PRODUCING SAME Michael Pantoliano, Robert C Ladner assigned to Genex Corporation Proteins, such as enzymes having enhanced stability are designed through the use of a computer method. The method identifies amino acid residues of a protein which may be replaced with a cysteine residue in order to promote the formation of a protein-stabilizing disulfide bond. The computer-designed, stabilized proteins can be produced using recombinant DNA technology.
4908312 4909941 PROCESS FOR PRODUCING PHENYLANANINE Akio Ozaki, Ryoichi Katsumata, Tetsuo Oka, Machida, Japan assigned to Kyowa Hakko Kogyo Co Ltd The invention relates to a bacterial process for producing phenylalanine. The process utilizes a Corynebacterium or Brevibacterium host which is transformed with a recombinant DNA. The recombinant D N A harbors a D N A containing a gene which encodes chorismate mutase or prephenate dehydratase. The transformed microorganism is then cultured in order to accumulate phenylalanine in the culture medium and the phenylalanine is recovered therefrom.
4908440 2',3'-DIDEOXY-2' FLUOROARABINOPYRIMIDINE NUCLEOSIDES Roman Z Sterzycki, Muzammil M Mansuri, John C Martin assigned to Bristol Myers Company There are disclosed novel 2',3'-dideoxy-2' fluoronucleosides and 2'-3'-dideoxy-2' 3"didehydro-2' fluoronucleosides and processes for their preparation. The compounds so produced exhibit therapeutically useful antiviral and, more particularly, anti-HIV effects.
HIGH PERFORMANCE CHROMATOGRAPHY PHASE
LIQUID MOBILE
Dick J Poll, David R K Harding, William S Hancock, Palmerston North, New Zealand There is described a mobile phase for use in the purification of proteins using reversed phase HPLC on a preparative scale. The mobile phase is an aqueous solution containing 0.005 to IM of a physiologicially acceptable acid of the general formula YCOOH where Y is a polar electron withdrawing group capable of hydrogen bonding silanol groups. The solution also contains up to 95% by volume of a physilogically acceptable organic solvent. A preparative method of purifying proteins such as HSA or BSA and recombinant deoxyribonucleic proteins using reversed phased PHLC is also described.
4910016 NOVEL BACILLUS THURINGIENSIS ISOLATE Frank H Gaertner, George G Soares, Jewel Payne assigned to Mycogen Corporation The subject invention concerns a novel and useful insecticide with activity against insect pests of the order Coleoptera and the order Lepidoptera. Pests in the order Coleoptera do heavy damage