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4912035 Method for minimizing interference by reductants when detecting cells in biological fluids
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PATENT ABSTRACTS tors comprising administering to the mammal a receptor probe or a weight control agent for inhibiting weight gain. The receptor probe or weight control agent may comprise certain azine, thiosemicarbazone, or N, N'-disubstituted thiourea derivatives of nonpeptide opioid antagonists.
4910297 ALPHA-AMYLASE
INHIBITOR
Urszula Zawistowska, Winnipeg, Canada assigned to ABI Biotechnology Inc A novel procedure for the preparation of a protein which is an inhibitor of alpha-amylase II is described. The protein may be prepared by extracting barley meal with a Tris-HC1 buffer and purifying the crude inhibitor thus obtained by a chromatographic procedure. Alternatively, the protein may be prepared by recombinant DNA techniques. The protein can be applied as an additive to sprout-damaged wheat flour which can then be used to provide improved quality bread.
4912036 RAPID DIFFERENTIATION OF BACTERIA USING CYCLIC POLYPEPTIDE ANTIBIOTICS Peggy W Cichanowicz, Robert T Belly assigned to Eastman Kodak Company Rapid differentiation between viable grampositive and gram-negative bacteria is accomplished with certain polypeptide antibiotics which are used in combination with a compound which is normally reducible by the bacteria. The antibiotics selectively inhibit the reduction of the reducible compound by gram-positive bacteria but do not substantially affect the reducing capacity of the gram-negative bacteria. The particular antibiotics useful are cyclic polypeptides which affect the function of the cytoplasmic membrane of bacteria. A particular polypeptide antibiotic, polymixin B, will distinguish Proteus bacteria from other gram-negative genera.
4912037 CONTAINER FOR MICROORGANISM CULTURE MEDIA Jean Lemonnier, Le Vesinet, France assigned to Millipore S A
4912035 METHOD FOR MINIMIZING INTERFERENCE BY REDUCTANTS WHEN DETECTING CELLS IN BIOLOGICAL FLUIDS Robert T Belly, Sheryl S Sullivan, Eric R Schmittou assigned to Eastman Kodak Company A method for the determination of cells in a sample is disclosed. The sample is typically urine and the method is useful in removing interfering reductants from the sample. The method comprises the steps of: (1) separating the cells from the sample, (2) washing the separated cells with: (a) an iron(III) chelate solution and (b) a nonionic surfactant solution and (3) contacting the washed cells with a redox reagent so as to produce a detectable change due to the presence of the cells.
A container for one or more micro-organism culture mediums has a substantially cylindrical or frustoconical casing open at both ends. A transverse perforated support grid is joined to an inside wall of the casing at its circular edge. A base is fitted to a lower end of the casing and a lid is fitted in a liquid-tight manner onto the upper end of the casing. A culture medium at a raised temperature can thus be poured into the container while it is resting upside down on its lid with the base removed.
4912041 DETERMINATION
OF AMYLASE
Mark Batchelor, Stephen Williams, Mokina Green, Reading, United Kingdom assigned to Medisense Inc A method of assay for the presence of alphaamylase in a sample employs a derivatized alphaoligosaccharide which can be hydrolyzed by an enzyme mix including alpha-amylase to yield an
PATENT ABSTRACTS tors comprising administering to the mammal a receptor probe or a weight control agent for inhibiting weight gain. The receptor probe or weight control agent may comprise certain azine, thiosemicarbazone, or N, N'-disubstituted thiourea derivatives of nonpeptide opioid antagonists.
4910297 ALPHA-AMYLASE
INHIBITOR
Urszula Zawistowska, Winnipeg, Canada assigned to ABI Biotechnology Inc A novel procedure for the preparation of a protein which is an inhibitor of alpha-amylase II is described. The protein may be prepared by extracting barley meal with a Tris-HC1 buffer and purifying the crude inhibitor thus obtained by a chromatographic procedure. Alternatively, the protein may be prepared by recombinant DNA techniques. The protein can be applied as an additive to sprout-damaged wheat flour which can then be used to provide improved quality bread.
4912036 RAPID DIFFERENTIATION OF BACTERIA USING CYCLIC POLYPEPTIDE ANTIBIOTICS Peggy W Cichanowicz, Robert T Belly assigned to Eastman Kodak Company Rapid differentiation between viable grampositive and gram-negative bacteria is accomplished with certain polypeptide antibiotics which are used in combination with a compound which is normally reducible by the bacteria. The antibiotics selectively inhibit the reduction of the reducible compound by gram-positive bacteria but do not substantially affect the reducing capacity of the gram-negative bacteria. The particular antibiotics useful are cyclic polypeptides which affect the function of the cytoplasmic membrane of bacteria. A particular polypeptide antibiotic, polymixin B, will distinguish Proteus bacteria from other gram-negative genera.
4912037 CONTAINER FOR MICROORGANISM CULTURE MEDIA Jean Lemonnier, Le Vesinet, France assigned to Millipore S A
4912035 METHOD FOR MINIMIZING INTERFERENCE BY REDUCTANTS WHEN DETECTING CELLS IN BIOLOGICAL FLUIDS Robert T Belly, Sheryl S Sullivan, Eric R Schmittou assigned to Eastman Kodak Company A method for the determination of cells in a sample is disclosed. The sample is typically urine and the method is useful in removing interfering reductants from the sample. The method comprises the steps of: (1) separating the cells from the sample, (2) washing the separated cells with: (a) an iron(III) chelate solution and (b) a nonionic surfactant solution and (3) contacting the washed cells with a redox reagent so as to produce a detectable change due to the presence of the cells.
A container for one or more micro-organism culture mediums has a substantially cylindrical or frustoconical casing open at both ends. A transverse perforated support grid is joined to an inside wall of the casing at its circular edge. A base is fitted to a lower end of the casing and a lid is fitted in a liquid-tight manner onto the upper end of the casing. A culture medium at a raised temperature can thus be poured into the container while it is resting upside down on its lid with the base removed.
4912041 DETERMINATION
OF AMYLASE
Mark Batchelor, Stephen Williams, Mokina Green, Reading, United Kingdom assigned to Medisense Inc A method of assay for the presence of alphaamylase in a sample employs a derivatized alphaoligosaccharide which can be hydrolyzed by an enzyme mix including alpha-amylase to yield an