- Email: [email protected]
4950593 Improved method for assaying proteolytic enzymes
Enzymes and Enzyme Systems gents and proteinases. Therefore, they can be effectively utilized not only as an additive for clothing detergents, but also as a biomass and in other fields.
4945043 PROCESS AND REAGENT FOR THE DETERMINATION OF ALPHA-AMYLASE
4948726
Marti Gerber, Weilheim Unterhausen, Federal Republic Of Germany assigned to Boehringer Mannheim GmbH
ENZYME IMMUNOASSAY BASED ON MEMBRANE SEPARATION OF ANTIGEN-ANTIBODY COMPLEXES
The present invention provides a process for the determination of alpha-amylase by cleavage of a substrate in the presence of one or more auxiliary enzymes and measurement of a cleavage product, wherein the reaction is carried out in the presence of monoclonal antibodies against alpha-amylase. The present invention also provides a reagent for the determination of alphaamylase containing a substrate, one or more auxiliary enzymes and a system for the measurement of a cleavage product, wherein it also contains at least one monoclonal antibody against alpha-amylase.
Claude C Longofia An enzyme immunoassay based on membrane separation of antigen-antibody complexes wherein human or animal body fluid specimens containing an antigen are mixed with an enzymeconjugated antibody specific for the antigen under test. Following incubation the antigenantibody-conjugate mixture is passed through a filter membrane having an electrostatic charge providing an affinity for retaining antigenantibody-conjugate complexes while not retaining free antibody-conjugate. Following washing of the filter membrane to remove free antibody-conjugate remaining thereon an enzyme substrate-chromogen reagent solution is applied to the filter membrane, which reacts with filter-bound antibody-conjugate and develops a visible or fluorogenic color indicative of the presence of antibody-conjugate.
4945053 NOVEL ALKALINE CELLULASES AND A MICROORGANISM FOR PRODUCING THE SAME Susum Ito, Tomokazu Sato, Katsuya Ozaki, Shitsuw Shikata, Kikuhiko Okamoto, Shigeo Inoue, Kenzo Koike, Yuichi Ota, Akira Takei, Utsunomiya, Japan assigned to Kao Corporation
4950593
Novel alkaline cellulase K, CMCase I and CMCase II are obtained by isolation from a culture product of Bacillus sp KSM-635. These enzymes stably work in a wide range including an alkaline side, and their activity is shown even at low temperatures. Further, they have a strong resistance to surface active agents, chelating a-
IMPROVED METHOD FOR ASSAYING PROTEOLYTIC ENZYMES Michael Matta, Raymond E O'Bear assigned to Vitek Systems Inc
297
298
PATENT ABSTRACTS
A reagent for proteolytic enzyme assays has the general formula See Patent f o r Chemical Structure where RCO- is an enzyme reactive acyl, such as an amino acid, peptide or substituted amino acid or peptide. The reagent may be hydrolysed by proteolytic enzymes and developed to form a distinctive color. The reagent may be formed by reacting RCOOH with N-hydroxysuccinimide to form the acyl N-hydroxysuccinimide ester. The ester may then be reacted to form the reagent.
The present invention provides a stabilized sarcosine oxidase preparation which contains creatineamidinohydrolasecovalently bound to a water-soluble polysaccharide.
4952493 PEPTIDE SUBSTRATES FOR DETECTING VIRUS-SPECIFIED PROTEASE ACTIVITY Charles A Kettner, Bruce D Korant assigned to E I du Pont de Nemours and Company
4950596 STABILIZATION OF INTRACELLULAR ENZYMES Roberta C Cheng, Norman Moll, Robert A Houtchens, Karen M McCoy assigned to The Dow Chemical Company The subject invention concerns a process for stabilizing intact or ruptured microbial cells having glucose isomerase associated therewith. Specifically exemplified is a process for stabilizingglucose isomerase producing cells of a microorganism belonging to the genus Ampullariella. In the invention process the whole or ruptured microbial cells are contacted with a partially carboxyalkylated- or partially phosphonoalkylated-cationic polyelectrolyte, for example, a partially carboxymethylated polyethyleneimine to flocculate and stabilize the cells. The flocculated ceils are further stabilized by encapsulation with a partially carboxyalkylatedor partially phosphonoalkylated-cationic polyelectrolyte. The encapsulation can be done prior to or after the flocculated cells are crosslinked. The net effect is manifested by a dramatic increase in the half-life of the glucose isomerase.
4950609 STABILIZED SARCOSINE OXIDASE PREPARATION Wilhelm Tischer, Manfred Gloger, Josef Heinle, Peissenberg, Federal Republic Of Germany assigned to Boehringer Mannheim GMBH
A method for preparing selected peptide substrates for detecting the activity of virusspecified proteases is provided. Specific tetrapeptide substrates are disclosed which are conjugates of protease-cleavable indicator groups and peptide sequences resembling picornavirus protease cleavage recognition sites.
4952505 FERMENTATION OF T R I C H O D E R M A REESEI AND A P P A R A T U S THEREFOR Michael Cho assigned to Florida State University A process for producing enzyme cellulases by the fermentation of Trichoderma reesei in an aqueous nutrient medium containing assimilable sources of cellulose, nitrogen, phosphate, magnesium and iron in the presence of an oxygen containing atmosphere and a fermentation apparatus for the aerobic fermentation of microorganisms in a liquid medium at a pressure in excess of about 7 atmospheres. The method comprises fermenting the Trichoderma reesei at a temperature of between about 26 degrees C. and 31 degrees C. while maintaining the oxygen containing atmosphere at a pressure of about 1 atmosphere until the Trichoderma reesei enter the late stationary growth phase, thereafter, gradually and steadily increasing the pressure of the oxygen containing atmosphere until it is in excess of about 7 atmospheres and culturing the Trichoderma reesei at said increased pressure and at a temperature of between about 40 degrees C. and about 60 degrees C., thereby resulting in the production of enzyme cellulases by the Trichoderma reesei, and recovering the enzyme cellulases.
4945043 PROCESS AND REAGENT FOR THE DETERMINATION OF ALPHA-AMYLASE
4948726
Marti Gerber, Weilheim Unterhausen, Federal Republic Of Germany assigned to Boehringer Mannheim GmbH
ENZYME IMMUNOASSAY BASED ON MEMBRANE SEPARATION OF ANTIGEN-ANTIBODY COMPLEXES
The present invention provides a process for the determination of alpha-amylase by cleavage of a substrate in the presence of one or more auxiliary enzymes and measurement of a cleavage product, wherein the reaction is carried out in the presence of monoclonal antibodies against alpha-amylase. The present invention also provides a reagent for the determination of alphaamylase containing a substrate, one or more auxiliary enzymes and a system for the measurement of a cleavage product, wherein it also contains at least one monoclonal antibody against alpha-amylase.
Claude C Longofia An enzyme immunoassay based on membrane separation of antigen-antibody complexes wherein human or animal body fluid specimens containing an antigen are mixed with an enzymeconjugated antibody specific for the antigen under test. Following incubation the antigenantibody-conjugate mixture is passed through a filter membrane having an electrostatic charge providing an affinity for retaining antigenantibody-conjugate complexes while not retaining free antibody-conjugate. Following washing of the filter membrane to remove free antibody-conjugate remaining thereon an enzyme substrate-chromogen reagent solution is applied to the filter membrane, which reacts with filter-bound antibody-conjugate and develops a visible or fluorogenic color indicative of the presence of antibody-conjugate.
4945053 NOVEL ALKALINE CELLULASES AND A MICROORGANISM FOR PRODUCING THE SAME Susum Ito, Tomokazu Sato, Katsuya Ozaki, Shitsuw Shikata, Kikuhiko Okamoto, Shigeo Inoue, Kenzo Koike, Yuichi Ota, Akira Takei, Utsunomiya, Japan assigned to Kao Corporation
4950593
Novel alkaline cellulase K, CMCase I and CMCase II are obtained by isolation from a culture product of Bacillus sp KSM-635. These enzymes stably work in a wide range including an alkaline side, and their activity is shown even at low temperatures. Further, they have a strong resistance to surface active agents, chelating a-
IMPROVED METHOD FOR ASSAYING PROTEOLYTIC ENZYMES Michael Matta, Raymond E O'Bear assigned to Vitek Systems Inc
297
298
PATENT ABSTRACTS
A reagent for proteolytic enzyme assays has the general formula See Patent f o r Chemical Structure where RCO- is an enzyme reactive acyl, such as an amino acid, peptide or substituted amino acid or peptide. The reagent may be hydrolysed by proteolytic enzymes and developed to form a distinctive color. The reagent may be formed by reacting RCOOH with N-hydroxysuccinimide to form the acyl N-hydroxysuccinimide ester. The ester may then be reacted to form the reagent.
The present invention provides a stabilized sarcosine oxidase preparation which contains creatineamidinohydrolasecovalently bound to a water-soluble polysaccharide.
4952493 PEPTIDE SUBSTRATES FOR DETECTING VIRUS-SPECIFIED PROTEASE ACTIVITY Charles A Kettner, Bruce D Korant assigned to E I du Pont de Nemours and Company
4950596 STABILIZATION OF INTRACELLULAR ENZYMES Roberta C Cheng, Norman Moll, Robert A Houtchens, Karen M McCoy assigned to The Dow Chemical Company The subject invention concerns a process for stabilizing intact or ruptured microbial cells having glucose isomerase associated therewith. Specifically exemplified is a process for stabilizingglucose isomerase producing cells of a microorganism belonging to the genus Ampullariella. In the invention process the whole or ruptured microbial cells are contacted with a partially carboxyalkylated- or partially phosphonoalkylated-cationic polyelectrolyte, for example, a partially carboxymethylated polyethyleneimine to flocculate and stabilize the cells. The flocculated ceils are further stabilized by encapsulation with a partially carboxyalkylatedor partially phosphonoalkylated-cationic polyelectrolyte. The encapsulation can be done prior to or after the flocculated cells are crosslinked. The net effect is manifested by a dramatic increase in the half-life of the glucose isomerase.
4950609 STABILIZED SARCOSINE OXIDASE PREPARATION Wilhelm Tischer, Manfred Gloger, Josef Heinle, Peissenberg, Federal Republic Of Germany assigned to Boehringer Mannheim GMBH
A method for preparing selected peptide substrates for detecting the activity of virusspecified proteases is provided. Specific tetrapeptide substrates are disclosed which are conjugates of protease-cleavable indicator groups and peptide sequences resembling picornavirus protease cleavage recognition sites.
4952505 FERMENTATION OF T R I C H O D E R M A REESEI AND A P P A R A T U S THEREFOR Michael Cho assigned to Florida State University A process for producing enzyme cellulases by the fermentation of Trichoderma reesei in an aqueous nutrient medium containing assimilable sources of cellulose, nitrogen, phosphate, magnesium and iron in the presence of an oxygen containing atmosphere and a fermentation apparatus for the aerobic fermentation of microorganisms in a liquid medium at a pressure in excess of about 7 atmospheres. The method comprises fermenting the Trichoderma reesei at a temperature of between about 26 degrees C. and 31 degrees C. while maintaining the oxygen containing atmosphere at a pressure of about 1 atmosphere until the Trichoderma reesei enter the late stationary growth phase, thereafter, gradually and steadily increasing the pressure of the oxygen containing atmosphere until it is in excess of about 7 atmospheres and culturing the Trichoderma reesei at said increased pressure and at a temperature of between about 40 degrees C. and about 60 degrees C., thereby resulting in the production of enzyme cellulases by the Trichoderma reesei, and recovering the enzyme cellulases.