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4952493 Peptide substrates for detecting virus-specified protease activity
298
PATENT ABSTRACTS
A reagent for proteolytic enzyme assays has the general formula See Patent f o r Chemical Structure where RCO- is an enzyme reactive acyl, such as an amino acid, peptide or substituted amino acid or peptide. The reagent may be hydrolysed by proteolytic enzymes and developed to form a distinctive color. The reagent may be formed by reacting RCOOH with N-hydroxysuccinimide to form the acyl N-hydroxysuccinimide ester. The ester may then be reacted to form the reagent.
The present invention provides a stabilized sarcosine oxidase preparation which contains creatineamidinohydrolasecovalently bound to a water-soluble polysaccharide.
4952493 PEPTIDE SUBSTRATES FOR DETECTING VIRUS-SPECIFIED PROTEASE ACTIVITY Charles A Kettner, Bruce D Korant assigned to E I du Pont de Nemours and Company
4950596 STABILIZATION OF INTRACELLULAR ENZYMES Roberta C Cheng, Norman Moll, Robert A Houtchens, Karen M McCoy assigned to The Dow Chemical Company The subject invention concerns a process for stabilizing intact or ruptured microbial cells having glucose isomerase associated therewith. Specifically exemplified is a process for stabilizingglucose isomerase producing cells of a microorganism belonging to the genus Ampullariella. In the invention process the whole or ruptured microbial cells are contacted with a partially carboxyalkylated- or partially phosphonoalkylated-cationic polyelectrolyte, for example, a partially carboxymethylated polyethyleneimine to flocculate and stabilize the cells. The flocculated ceils are further stabilized by encapsulation with a partially carboxyalkylatedor partially phosphonoalkylated-cationic polyelectrolyte. The encapsulation can be done prior to or after the flocculated cells are crosslinked. The net effect is manifested by a dramatic increase in the half-life of the glucose isomerase.
4950609 STABILIZED SARCOSINE OXIDASE PREPARATION Wilhelm Tischer, Manfred Gloger, Josef Heinle, Peissenberg, Federal Republic Of Germany assigned to Boehringer Mannheim GMBH
A method for preparing selected peptide substrates for detecting the activity of virusspecified proteases is provided. Specific tetrapeptide substrates are disclosed which are conjugates of protease-cleavable indicator groups and peptide sequences resembling picornavirus protease cleavage recognition sites.
4952505 FERMENTATION OF T R I C H O D E R M A REESEI AND A P P A R A T U S THEREFOR Michael Cho assigned to Florida State University A process for producing enzyme cellulases by the fermentation of Trichoderma reesei in an aqueous nutrient medium containing assimilable sources of cellulose, nitrogen, phosphate, magnesium and iron in the presence of an oxygen containing atmosphere and a fermentation apparatus for the aerobic fermentation of microorganisms in a liquid medium at a pressure in excess of about 7 atmospheres. The method comprises fermenting the Trichoderma reesei at a temperature of between about 26 degrees C. and 31 degrees C. while maintaining the oxygen containing atmosphere at a pressure of about 1 atmosphere until the Trichoderma reesei enter the late stationary growth phase, thereafter, gradually and steadily increasing the pressure of the oxygen containing atmosphere until it is in excess of about 7 atmospheres and culturing the Trichoderma reesei at said increased pressure and at a temperature of between about 40 degrees C. and about 60 degrees C., thereby resulting in the production of enzyme cellulases by the Trichoderma reesei, and recovering the enzyme cellulases.
PATENT ABSTRACTS
A reagent for proteolytic enzyme assays has the general formula See Patent f o r Chemical Structure where RCO- is an enzyme reactive acyl, such as an amino acid, peptide or substituted amino acid or peptide. The reagent may be hydrolysed by proteolytic enzymes and developed to form a distinctive color. The reagent may be formed by reacting RCOOH with N-hydroxysuccinimide to form the acyl N-hydroxysuccinimide ester. The ester may then be reacted to form the reagent.
The present invention provides a stabilized sarcosine oxidase preparation which contains creatineamidinohydrolasecovalently bound to a water-soluble polysaccharide.
4952493 PEPTIDE SUBSTRATES FOR DETECTING VIRUS-SPECIFIED PROTEASE ACTIVITY Charles A Kettner, Bruce D Korant assigned to E I du Pont de Nemours and Company
4950596 STABILIZATION OF INTRACELLULAR ENZYMES Roberta C Cheng, Norman Moll, Robert A Houtchens, Karen M McCoy assigned to The Dow Chemical Company The subject invention concerns a process for stabilizing intact or ruptured microbial cells having glucose isomerase associated therewith. Specifically exemplified is a process for stabilizingglucose isomerase producing cells of a microorganism belonging to the genus Ampullariella. In the invention process the whole or ruptured microbial cells are contacted with a partially carboxyalkylated- or partially phosphonoalkylated-cationic polyelectrolyte, for example, a partially carboxymethylated polyethyleneimine to flocculate and stabilize the cells. The flocculated ceils are further stabilized by encapsulation with a partially carboxyalkylatedor partially phosphonoalkylated-cationic polyelectrolyte. The encapsulation can be done prior to or after the flocculated cells are crosslinked. The net effect is manifested by a dramatic increase in the half-life of the glucose isomerase.
4950609 STABILIZED SARCOSINE OXIDASE PREPARATION Wilhelm Tischer, Manfred Gloger, Josef Heinle, Peissenberg, Federal Republic Of Germany assigned to Boehringer Mannheim GMBH
A method for preparing selected peptide substrates for detecting the activity of virusspecified proteases is provided. Specific tetrapeptide substrates are disclosed which are conjugates of protease-cleavable indicator groups and peptide sequences resembling picornavirus protease cleavage recognition sites.
4952505 FERMENTATION OF T R I C H O D E R M A REESEI AND A P P A R A T U S THEREFOR Michael Cho assigned to Florida State University A process for producing enzyme cellulases by the fermentation of Trichoderma reesei in an aqueous nutrient medium containing assimilable sources of cellulose, nitrogen, phosphate, magnesium and iron in the presence of an oxygen containing atmosphere and a fermentation apparatus for the aerobic fermentation of microorganisms in a liquid medium at a pressure in excess of about 7 atmospheres. The method comprises fermenting the Trichoderma reesei at a temperature of between about 26 degrees C. and 31 degrees C. while maintaining the oxygen containing atmosphere at a pressure of about 1 atmosphere until the Trichoderma reesei enter the late stationary growth phase, thereafter, gradually and steadily increasing the pressure of the oxygen containing atmosphere until it is in excess of about 7 atmospheres and culturing the Trichoderma reesei at said increased pressure and at a temperature of between about 40 degrees C. and about 60 degrees C., thereby resulting in the production of enzyme cellulases by the Trichoderma reesei, and recovering the enzyme cellulases.