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4963487 Method for deletion of a gene from a bacteria
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PATENT ABSTRACTS The present invention provides a recombinant DNA, wherein it contains the consensus sequence: See Patentfor Chemical Structure and a promoter which has the DNA sequence GGN 10GC. The present invention also provides a process for the isolation of this recombinant DNA, wherein a DNA sequence containing the consensus sequence is identified from a gene bank of a micro-organism, which contains a gene which is repressible by oxygen and inducible by formate under anaerobic conditions, isolated and combined with an appropriate promotor according to known methods. This recombinant gene can be used for the inducible and repressible expression of a foreign gene by bringing about the induction by formate under anaerobic conditions and the repression by oxygen.
4963483 METHOD FOR PRODUCING HEPATITIS B VIRUS PROTEINS IN YEAST Ronald W Ellis, Arp Hagopian, Peter J Kniskern, Donna L Montgomery assigned to Merck & Co Inc The hepatitis B virus preS2 antigen gene linked in one contiguous reading frame to the hepatitis B virus surface antigen gene has been expressed in Saccharomyces cerevisiae utilizing an optimized plasmid construction. The expressed protein aggregates into a particulate form which displays the major antigenic sites encoded by both domains, thereby highlighting the utility of yeast as a host for the high level expression of the preS2 as well as the S domain. This protein is useful in in vitro diagnostic systems and as a vaccine for the treatment and prevention of hepatitis B virus-induced diseases and/or infections.
4963484 GENETICALLY ENGINEERED POLYPEPTIDES WITH DETERMINANTS OF THE HUMAN DF3 BREAST CARCINOMA-ASSOCIATED ANTIGEN
immunologically reactive with monoclonal antibody against the human DF3 breast carcinomaassociated antigen. The nucleotide sequence is also useful as a probe to reveal restriction fragment length polymorphisms in human DNA.
4963487 METHOD FOR DELETION OF A GENE FROM A BACTERIA Paul R Schimmel assigned to Massachusetts Institute of Technology Disclosed is a method and linear DNA fragments for use in the deletion ofa gene from a bacteria with a single step procedure that is applicable to any essential or nonessential gene which has been cloned. Chromosomal deletions are constructed by transformation of a cell strain with linear DNA fragments containing a locus for resistance to an antibiotic, or any other gene allowing for rapid phenotypic selection, flanked by sequences homologous to closely spaced regions on the cell chromosome on either side of the gene to be deleted, in combination with the immediate subsequent deletion or inactivation of the recA gene. By selecting for a doublecrossover event between the homologous sequences, shown by the antibiotic resistance or other detectable phenotype, a chromosome disruption can be selected for which has effectively deleted an entire gene. Inactivation or deletion of the recA gene prevents recombination or incorporation of extrachromosomal elements from occurring, thereby resulting in a bacterial strain which is useful for screening for functional activity or production of genetically engineered proteins in the absence of specific contaminants.
49~4~ DNA SEQUENCES, RECOMBINANT DNA MOLECULES AND PROCESS FOR THE PREPARATION OF THE ENZYME MUTAROTASE FROM ACINETOBACTER CALCOACETICUS
Donald W Kufe assigned to Dana-Farber Cancer Institute Inc
Christiane Gatz, Joachim Altschmied, Hans G Gassen, Wolfgang Hillen, Marl, Federal Republic Of Germany assigned to Merck Patent Gesellschaft mit beschrankter Haftung
A carbohydrate-frec polypeptide coded for by a human DNA sequence of 309 nucleotides is
The invention relates to DNA sequences, recombinant DNA molecules and transformed host or-
PATENT ABSTRACTS The present invention provides a recombinant DNA, wherein it contains the consensus sequence: See Patentfor Chemical Structure and a promoter which has the DNA sequence GGN 10GC. The present invention also provides a process for the isolation of this recombinant DNA, wherein a DNA sequence containing the consensus sequence is identified from a gene bank of a micro-organism, which contains a gene which is repressible by oxygen and inducible by formate under anaerobic conditions, isolated and combined with an appropriate promotor according to known methods. This recombinant gene can be used for the inducible and repressible expression of a foreign gene by bringing about the induction by formate under anaerobic conditions and the repression by oxygen.
4963483 METHOD FOR PRODUCING HEPATITIS B VIRUS PROTEINS IN YEAST Ronald W Ellis, Arp Hagopian, Peter J Kniskern, Donna L Montgomery assigned to Merck & Co Inc The hepatitis B virus preS2 antigen gene linked in one contiguous reading frame to the hepatitis B virus surface antigen gene has been expressed in Saccharomyces cerevisiae utilizing an optimized plasmid construction. The expressed protein aggregates into a particulate form which displays the major antigenic sites encoded by both domains, thereby highlighting the utility of yeast as a host for the high level expression of the preS2 as well as the S domain. This protein is useful in in vitro diagnostic systems and as a vaccine for the treatment and prevention of hepatitis B virus-induced diseases and/or infections.
4963484 GENETICALLY ENGINEERED POLYPEPTIDES WITH DETERMINANTS OF THE HUMAN DF3 BREAST CARCINOMA-ASSOCIATED ANTIGEN
immunologically reactive with monoclonal antibody against the human DF3 breast carcinomaassociated antigen. The nucleotide sequence is also useful as a probe to reveal restriction fragment length polymorphisms in human DNA.
4963487 METHOD FOR DELETION OF A GENE FROM A BACTERIA Paul R Schimmel assigned to Massachusetts Institute of Technology Disclosed is a method and linear DNA fragments for use in the deletion ofa gene from a bacteria with a single step procedure that is applicable to any essential or nonessential gene which has been cloned. Chromosomal deletions are constructed by transformation of a cell strain with linear DNA fragments containing a locus for resistance to an antibiotic, or any other gene allowing for rapid phenotypic selection, flanked by sequences homologous to closely spaced regions on the cell chromosome on either side of the gene to be deleted, in combination with the immediate subsequent deletion or inactivation of the recA gene. By selecting for a doublecrossover event between the homologous sequences, shown by the antibiotic resistance or other detectable phenotype, a chromosome disruption can be selected for which has effectively deleted an entire gene. Inactivation or deletion of the recA gene prevents recombination or incorporation of extrachromosomal elements from occurring, thereby resulting in a bacterial strain which is useful for screening for functional activity or production of genetically engineered proteins in the absence of specific contaminants.
49~4~ DNA SEQUENCES, RECOMBINANT DNA MOLECULES AND PROCESS FOR THE PREPARATION OF THE ENZYME MUTAROTASE FROM ACINETOBACTER CALCOACETICUS
Donald W Kufe assigned to Dana-Farber Cancer Institute Inc
Christiane Gatz, Joachim Altschmied, Hans G Gassen, Wolfgang Hillen, Marl, Federal Republic Of Germany assigned to Merck Patent Gesellschaft mit beschrankter Haftung
A carbohydrate-frec polypeptide coded for by a human DNA sequence of 309 nucleotides is
The invention relates to DNA sequences, recombinant DNA molecules and transformed host or-