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4968602 Solution-phase single hybridization assay for detecting polynucleotide sequences
PATENT ABSTRACTS is contained and retained within the pores of the polymeric material and is adapted in use to interact with a reactive species and can be made by depositing and retaining the gel or a material adapted in use to form the gel within the pores of the porous polymeric material. The high porosity of the porous polymeric material in combination with the retention of the gel within the pores permit high loading capacities, particularly in the area of peptide synthesis to be achieved. The substrate can be employed in chemical synthesis, chromatography techniques, ion exchange and separation techniques.
4966792 METHOD OF PRODUCING GRADIENT GEL MEDIUM MEMBRANE FOR ELECTROPHORESIS Fumitak Terai, Kimio Yukawa, Mineo Suefuji, Kanagawa, Japan assigned to Fuji Photo Film Co Ltd A method for producing gradient gel medium membrane for electrophoresis for determining the base sequence of DNA or DNA partially decomposed material providing an improved productivity. High and low concentration monomer solutions are mixed with a predetermined quantity of polymerizing reaction initiator solution by a static mixer to prepare a gel forming solution for coating on a continuously moving web. The flow-rate ratio of the high and low concentration monomer solutions is gradually changed so as to vary the concentration of the monomer in the gel forming solution alternatively from low to high and from high to low along the web.
4966848 ISOLATION, PURIFICATION, CHARACTERIZATION, CLONING AND SEQUENCING OF N ALPHAACETYLTRANSFERASE John A Smith, Fang-Jen S Lee assigned to The General Hospital Corporation This invention is directed to N alphaacetyltransferase with a molecular weight of about 180,000 daltons, said N alphaacetyltransferase being composed of two subunit peptides having molecular weights of about 95,000 each, having enzyme activity greater than
99
100 wherein one unit of activity is defined as 1 pmol of acetyl residues incorporated into adrenocorticotropic hormone (ACTH) (amino acids 1-24) under standard assay conditions. This invention is further directed to a method for purifying the N alpha-acetyltransferase.
4966853 CELL CULTURING
APPARATUS
Shoichi Matsuda, Akira Suzuki, Tatsuo Kaise, Tokyo, Japan assigned to Kirin Beer Kabushiki Kaisha A cell culturing apparatus and a cell culturing method are disclosed. A rack supporting apparatus includes a loop tracking and a plurality of culturing racks connected one after another in series. Each of the culturing racks accommodates culturing containers therein for cell culturing during their travel on the loop tracking. Each of the racks is accessible, through conveyors, to a container handling station where culture medium is filled in the culturing containers and cell inoculation is carried out. The culturing containers processed in the container handling station are automatically accommodated into the rack by an infeed station for starting cell culturing, and the culturing containers in which cell culturing have been performed in the rack are automatically discharged therefrom by a discharge station.
4968602 SOLUTION-PHASE SINGLE HYBRIDIZATION ASSAY FOR DETECTING POLYNUCLEOTIDE SEQUENCES Nanibhushan Dattagupta assigned to Molecular Diagnostics Inc A process for determining the presence of a particular nucleic acid sequence in a test sample comprising (a) chemically modifying nucleic acids in the test sample either to introduce a label or a reactive site in a manner that supports their hybridizability, (b) contacting under hybridization conditions the chemically modified sample nucleic acids with a hybridizable nucleic acid probe which either, when the sample nucleic acids have been modified to introduce a label, carrys a reactive site or, when the sample nucleic acids have been modified to introduce a reactive site, is labeled, (c) contacting the solution resul-
PATENT ABSTRACTS
100
ting from step (b) with a immobilized form of a reactive partner to the reactive site to form a stable bond with the reactive site on the sample nucleic acids or the probe, respectively, (d) separating the resulting immobilized fraction from the remaining solution, and (e) determining the presence of the label in the separated immobilized fraction or a decrease in the label in the remaining solution.
4968612 CONTINUOUS FERMENTATION PROCESS FOR AROMATIC HYDROCARBON BIOCONVERSION Jih-Han Hsieh assigned to Celgene Corporation This invention provides a continuous bioconversion process in which a non-growth toluene substrate is bio-oxidized by a specific microbe mutant strain to accumulated extracellular muconic acid at a bioreactor production rate of at least about 5 grams of muconic acid per liter of fermentation medium per hour. Essential features of the invention process include a continuous feed of whole cell-containing fermentation broth from an auxiliary cell growth and enzyme induction fermentation zone into the main fermentation zone, and a purge stream of whole cell-containing fermentation broth from the main fermentation zone.
4968623 CELL CULTURE APPARATUS Joseph Franks, Middlesex, United Kingdom assigned to lon Tech Limited Cell Culture apparatus provides a surface for cell culture, the surface being formed from the group of diamond-like carbon, pyrolytic carbon, glassy carbon and turbostratic carbon. The surface may be part of the container for the culture medium or the surface of microcarriers, such as beads of glass or polymers.
4~01~ METHOD OF DETERMINING ABSORBED NUTRIMENT IN LIVING ORGANISMS Harald Skjervold, £521 s, Norway assigned to Havbrukskjemi A/S
A method of determining the amount of nutriment absorbed in living organisms comprising animals, fish and plants comprises adding to the animal, fish or plant food one or more elements from the lanthanide series as tracers. After the nutriment has been absorbed, a sample is taken from a localized part of the living organism, for example a fish scale, and the sample is analyzed by ICP (inductively coupled plasma) to determine the amount of tracer and thus the amount of absorbed nutriment in the living organism.
4970152 REAGENTS FOR DETERMINING PEPTIDOGLYCAN AND BETA-I,3GLUCAN Masaaki Ashida, Masakazu Tsuchiya, Yoshitsugu Sakata, Shuji Matsuura, Sapporo, Japan assigned to Wako Pure Chemical Industries Ltd A reagent comprising a fraction obtained from plasma of an insect such as silkworm larvae and capable of reacting specifically with beta-l,3glucan or peptidoglycan can be used for determining beta-1,3-glucan or peptidoglycan.
4970166 BIOREACTOR HAVING A GAS EXCHANGER Kei Moil, Kaminoge, Setagaya ku, Tokyo, Japan A bioreactor comprising a bioreactor tank, a plurality of transparent cylindrical bodies together making up a light radiator and arranged parallel to each other in the bioreactor tank, a plurality of optical conductors inserted into each of the transparent cylindrical bodies, a light source device for guiding into the optical conductors the visible light ray components of solar rays and/or artificial light rays, and a gasexchanger for supplying carbon dioxide CO2 to a micro-organisms suspension in the space between the transparent cylindrical bodies in the bioreactor. A part of the micro-organisms suspension in the bioreactor is returned to the bioreactor through the gas-exchanger, and carbon dioxide CO2 is supplied to the microorganisms suspension in the gas-exchanger. The gas-exchanger consists of silicone pipes having microscopic holes through which the returned micro-organisms suspension pass and a hermetically sealed tank into which the silicone
4966792 METHOD OF PRODUCING GRADIENT GEL MEDIUM MEMBRANE FOR ELECTROPHORESIS Fumitak Terai, Kimio Yukawa, Mineo Suefuji, Kanagawa, Japan assigned to Fuji Photo Film Co Ltd A method for producing gradient gel medium membrane for electrophoresis for determining the base sequence of DNA or DNA partially decomposed material providing an improved productivity. High and low concentration monomer solutions are mixed with a predetermined quantity of polymerizing reaction initiator solution by a static mixer to prepare a gel forming solution for coating on a continuously moving web. The flow-rate ratio of the high and low concentration monomer solutions is gradually changed so as to vary the concentration of the monomer in the gel forming solution alternatively from low to high and from high to low along the web.
4966848 ISOLATION, PURIFICATION, CHARACTERIZATION, CLONING AND SEQUENCING OF N ALPHAACETYLTRANSFERASE John A Smith, Fang-Jen S Lee assigned to The General Hospital Corporation This invention is directed to N alphaacetyltransferase with a molecular weight of about 180,000 daltons, said N alphaacetyltransferase being composed of two subunit peptides having molecular weights of about 95,000 each, having enzyme activity greater than
99
100 wherein one unit of activity is defined as 1 pmol of acetyl residues incorporated into adrenocorticotropic hormone (ACTH) (amino acids 1-24) under standard assay conditions. This invention is further directed to a method for purifying the N alpha-acetyltransferase.
4966853 CELL CULTURING
APPARATUS
Shoichi Matsuda, Akira Suzuki, Tatsuo Kaise, Tokyo, Japan assigned to Kirin Beer Kabushiki Kaisha A cell culturing apparatus and a cell culturing method are disclosed. A rack supporting apparatus includes a loop tracking and a plurality of culturing racks connected one after another in series. Each of the culturing racks accommodates culturing containers therein for cell culturing during their travel on the loop tracking. Each of the racks is accessible, through conveyors, to a container handling station where culture medium is filled in the culturing containers and cell inoculation is carried out. The culturing containers processed in the container handling station are automatically accommodated into the rack by an infeed station for starting cell culturing, and the culturing containers in which cell culturing have been performed in the rack are automatically discharged therefrom by a discharge station.
4968602 SOLUTION-PHASE SINGLE HYBRIDIZATION ASSAY FOR DETECTING POLYNUCLEOTIDE SEQUENCES Nanibhushan Dattagupta assigned to Molecular Diagnostics Inc A process for determining the presence of a particular nucleic acid sequence in a test sample comprising (a) chemically modifying nucleic acids in the test sample either to introduce a label or a reactive site in a manner that supports their hybridizability, (b) contacting under hybridization conditions the chemically modified sample nucleic acids with a hybridizable nucleic acid probe which either, when the sample nucleic acids have been modified to introduce a label, carrys a reactive site or, when the sample nucleic acids have been modified to introduce a reactive site, is labeled, (c) contacting the solution resul-
PATENT ABSTRACTS
100
ting from step (b) with a immobilized form of a reactive partner to the reactive site to form a stable bond with the reactive site on the sample nucleic acids or the probe, respectively, (d) separating the resulting immobilized fraction from the remaining solution, and (e) determining the presence of the label in the separated immobilized fraction or a decrease in the label in the remaining solution.
4968612 CONTINUOUS FERMENTATION PROCESS FOR AROMATIC HYDROCARBON BIOCONVERSION Jih-Han Hsieh assigned to Celgene Corporation This invention provides a continuous bioconversion process in which a non-growth toluene substrate is bio-oxidized by a specific microbe mutant strain to accumulated extracellular muconic acid at a bioreactor production rate of at least about 5 grams of muconic acid per liter of fermentation medium per hour. Essential features of the invention process include a continuous feed of whole cell-containing fermentation broth from an auxiliary cell growth and enzyme induction fermentation zone into the main fermentation zone, and a purge stream of whole cell-containing fermentation broth from the main fermentation zone.
4968623 CELL CULTURE APPARATUS Joseph Franks, Middlesex, United Kingdom assigned to lon Tech Limited Cell Culture apparatus provides a surface for cell culture, the surface being formed from the group of diamond-like carbon, pyrolytic carbon, glassy carbon and turbostratic carbon. The surface may be part of the container for the culture medium or the surface of microcarriers, such as beads of glass or polymers.
4~01~ METHOD OF DETERMINING ABSORBED NUTRIMENT IN LIVING ORGANISMS Harald Skjervold, £521 s, Norway assigned to Havbrukskjemi A/S
A method of determining the amount of nutriment absorbed in living organisms comprising animals, fish and plants comprises adding to the animal, fish or plant food one or more elements from the lanthanide series as tracers. After the nutriment has been absorbed, a sample is taken from a localized part of the living organism, for example a fish scale, and the sample is analyzed by ICP (inductively coupled plasma) to determine the amount of tracer and thus the amount of absorbed nutriment in the living organism.
4970152 REAGENTS FOR DETERMINING PEPTIDOGLYCAN AND BETA-I,3GLUCAN Masaaki Ashida, Masakazu Tsuchiya, Yoshitsugu Sakata, Shuji Matsuura, Sapporo, Japan assigned to Wako Pure Chemical Industries Ltd A reagent comprising a fraction obtained from plasma of an insect such as silkworm larvae and capable of reacting specifically with beta-l,3glucan or peptidoglycan can be used for determining beta-1,3-glucan or peptidoglycan.
4970166 BIOREACTOR HAVING A GAS EXCHANGER Kei Moil, Kaminoge, Setagaya ku, Tokyo, Japan A bioreactor comprising a bioreactor tank, a plurality of transparent cylindrical bodies together making up a light radiator and arranged parallel to each other in the bioreactor tank, a plurality of optical conductors inserted into each of the transparent cylindrical bodies, a light source device for guiding into the optical conductors the visible light ray components of solar rays and/or artificial light rays, and a gasexchanger for supplying carbon dioxide CO2 to a micro-organisms suspension in the space between the transparent cylindrical bodies in the bioreactor. A part of the micro-organisms suspension in the bioreactor is returned to the bioreactor through the gas-exchanger, and carbon dioxide CO2 is supplied to the microorganisms suspension in the gas-exchanger. The gas-exchanger consists of silicone pipes having microscopic holes through which the returned micro-organisms suspension pass and a hermetically sealed tank into which the silicone