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4977077 Integrated solid-phase immunoassay
PATENT ABSTRACTS Test compositions, indicators, and test device are provided which are capable of generating different hues at different analyte concentrations. The compositions are capable of generating a yellow hue in situ. Visual tests for clinically important analytes, such as glucose, are determined by use of two independent catalytic systems which are reactive with reduced nicotinamide adenine dinucleotide to produce a range of hues; the particular hue produced depending on the concentration of the analyte. The invention provides a test device for the determination of analyre, e.g. glucose, in body fluid which exhibits a rainbow of hues, the particular final hue produced depending on the analyte concentration.
289
4976865 METHOD
FOR THE SEPARATION OF BIOLOGICAL MACROMOLECULES BY CHROMATOGRAPHY
Victor Sanchez, Beatrice Biscans, Jean-Pierre Couderc, Jean-Pierre Riba, Ramonville Saint Agne, France assigned to Centre National de la Recherche Scientifique
4975377
A method for allowing biological macromolecules contained in a solution to be separated by selective absorption comprising supplying with a solution each stage (3, 4) of a column containing a selective chromatographic resin specific to the macromolecules to be separated, so as to fluidize the beds of resin at each stage, each stage being provided at its base with a perforated distribution system (5) characterized by a percentage of open sections between 0.2% and 10%, a mean diameter of the said open sections greater than approximately 300 microns and between 2Gm and 20Gm, where Gm is the mean granulometry of the resin.
CELL GROWTH CHAMBERS AND METHOD OF USE THEREOF
4976951
Marc E Key Growth chambers for anchorage-independent cell growth therein are formed of a gel matrix having a surface disallowing anchoragedependent cell growth over the full interior thereof. In a preferred form the chambers have a generally cylidnrical wall and an integral convex bottom wall forming an annular volume at the foot of the cylindrical wall which is substantially lower than the central portion to concentrate such anchorage-indpendent cells. The gel matrix is sufficiently permeable to permit passage of cell-growth nutrients and waste product solutes through said wall when the chambers are filled below the open end and submerged in a growth medium. Preferably the gel matrix is formed of 1% to 5% cross-linked polyacrylamide and from 99% to 95% water. In a preferred method of using the growth chambers, undifferentiated tumor cells and normal cells, including fibroblasts, are cultured together. Anchorageindependent tumor cells proliferate while anchorage-dependent cells are unable to grow without attachment. The method is useful for evaluating in vitro therapeutic agents to control tumor growth, normal cell growth or microspheres and generation of immunoglobulins from lymphocyte cells.
DENTAL CARIES DIAGNOSTIC AND LOCALIZATION TECHNIQUE Melvyn Rosenberg, Ilana Eli, Ervin Weiss, Ramat Gan 52526, Israel A method for detecting and localizing bacterial growth on a surface comprising the steps of: (a) applying a solid substrate to the surface; (b) removing said substrate from said surface and incubating said substrate for purposes of facilitating ultimate visualization of selective bacterial growth; and (c) observing the locus of bacterial growth on the substrate. The method is particularly effective in detecting and localizing cariogenic activity in the mouth, 4977077 INTEGRATED SOLID-PHASE IMMUNOASSAY That T Ngo, Raphael C Wong assigned to Bioprobe International The presence of an antigenic analyte ligand in a liquid sample is determined by (1) mixing the
PATENT ABSTRACTS
290
sample with a fluorescently labeled ligand immunologically complexed onto a solid phase supported antibody capable of exchanging the fluorescently labeled ligand with the analyte ligand in the sample and (2) measuring the fluorescence of the resulting liquid phase without measuring the fluorescence of the resulting solid phase and without separating the resulting solid phase from the resulting liquid phase.
4977080 CHEMILUMINESCENT METHODS AND A KIT THEREFOR INVOLVING A BETA-LACTAM
was characterized. Monocyte cytotoxicity inducing factor was found to be stable at pH 2 for one hour, unlike interferon-gamma, and was found to be more heat stable as well. Moreover, treatment of MCF with antisera to interferons gamma, alpha, or a combination of gamma and alpha failed to neutralize its biologic activity. MCF binds to Matrex Gel Red A. MCF eluted from this dye-ligand was found to have an apparent molecular weight of I 1,500 Daltons by gel filtration and 14,700 Daltons by SDSpolyacrylamide gel electrophoresis. MCF produced by hybridized Sezary cells appear to be neither interferon-gamma nor an altered molecular form of interferon-gamma, yet is a potent inducer of human monocyte cytotoxicity.
4977247 Dean Milbrath assigned to Minnesota Mining and Manufacturing Co Methods for the detection and measurement of compounds containing beta-lactam rings by chemiluminescence of the compounds in the presence of a chemiluminescent compound such as luminol, and for improving the sensitivity of assays using chemiluminescent reactions such as the luminol reaction utilizing compounds containing beta-lactam rings are described. A kit for conducting such chemiluminescent assays is also described.
IMMOBILIZED PROTEIN G VARIANTS AND THE USE THEREOF Stephen R Fahnestock, Timothy Lee, Marie H Wroble assigned to Genex Corporation Immobilized IgG binding proteins and the use thereof to effect affinity chromatography and separate the subclasses of IgG. Disclosed are cysteine-containing lgG binding proteins which have high binding capacity for human IgG and mouse monoclonal lgG.
4977245 METHODS AND COMPOSITIONS FOR INDUCING MONOCYTE CYTOTOXICITY C Michae Jones assigned to Board of Regents The University of Texas System The present disclosure relates to a new lymphokine molecule, referred to as Monocyte Cytotoxicity Inducing Factor (MCF), and its use as in cancer and other types of therapy. The disclosure further relates to the development of novel Sezary cell hybridomas which secrete MCF and thereby provide a ready source for MCF isolation and purification. Sezary'is Syndrome i a leukemic proliferation o f O K T 4 + lymphocytes. Sezary cells were isolated by differential centrifugation and fused to CEM.8aza.rC, an HGPRTase lacking clone of CEM. The hybrid cells were studied for their ability to produce soluble mediators of human monocyte cytotoxicity. The product of a single clone, FtF3, which bore the surface phenotype of Sezary cells,
4978608 DNA DETECTION
SYSTEM
Viola T Kung, Peter A Nagainis assigned to Molecular Devices Corporation Picogram amounts of DNA can be detected in a sample by the use of high affinity single-stranded DNA binding proteins. The assay is applicable not only to pure DNA samples but also to samples containing significant amounts of protein.
4978610 METHOD OF ASSAY EMPLOYING A MAGNETIC ELECTRODE Gordon C Forrest, Simon Rattle, Grenville Robinson, Hugh A O Hill, East Horsley, Surrey KT24 5DP, United Kingdom
289
4976865 METHOD
FOR THE SEPARATION OF BIOLOGICAL MACROMOLECULES BY CHROMATOGRAPHY
Victor Sanchez, Beatrice Biscans, Jean-Pierre Couderc, Jean-Pierre Riba, Ramonville Saint Agne, France assigned to Centre National de la Recherche Scientifique
4975377
A method for allowing biological macromolecules contained in a solution to be separated by selective absorption comprising supplying with a solution each stage (3, 4) of a column containing a selective chromatographic resin specific to the macromolecules to be separated, so as to fluidize the beds of resin at each stage, each stage being provided at its base with a perforated distribution system (5) characterized by a percentage of open sections between 0.2% and 10%, a mean diameter of the said open sections greater than approximately 300 microns and between 2Gm and 20Gm, where Gm is the mean granulometry of the resin.
CELL GROWTH CHAMBERS AND METHOD OF USE THEREOF
4976951
Marc E Key Growth chambers for anchorage-independent cell growth therein are formed of a gel matrix having a surface disallowing anchoragedependent cell growth over the full interior thereof. In a preferred form the chambers have a generally cylidnrical wall and an integral convex bottom wall forming an annular volume at the foot of the cylindrical wall which is substantially lower than the central portion to concentrate such anchorage-indpendent cells. The gel matrix is sufficiently permeable to permit passage of cell-growth nutrients and waste product solutes through said wall when the chambers are filled below the open end and submerged in a growth medium. Preferably the gel matrix is formed of 1% to 5% cross-linked polyacrylamide and from 99% to 95% water. In a preferred method of using the growth chambers, undifferentiated tumor cells and normal cells, including fibroblasts, are cultured together. Anchorageindependent tumor cells proliferate while anchorage-dependent cells are unable to grow without attachment. The method is useful for evaluating in vitro therapeutic agents to control tumor growth, normal cell growth or microspheres and generation of immunoglobulins from lymphocyte cells.
DENTAL CARIES DIAGNOSTIC AND LOCALIZATION TECHNIQUE Melvyn Rosenberg, Ilana Eli, Ervin Weiss, Ramat Gan 52526, Israel A method for detecting and localizing bacterial growth on a surface comprising the steps of: (a) applying a solid substrate to the surface; (b) removing said substrate from said surface and incubating said substrate for purposes of facilitating ultimate visualization of selective bacterial growth; and (c) observing the locus of bacterial growth on the substrate. The method is particularly effective in detecting and localizing cariogenic activity in the mouth, 4977077 INTEGRATED SOLID-PHASE IMMUNOASSAY That T Ngo, Raphael C Wong assigned to Bioprobe International The presence of an antigenic analyte ligand in a liquid sample is determined by (1) mixing the
PATENT ABSTRACTS
290
sample with a fluorescently labeled ligand immunologically complexed onto a solid phase supported antibody capable of exchanging the fluorescently labeled ligand with the analyte ligand in the sample and (2) measuring the fluorescence of the resulting liquid phase without measuring the fluorescence of the resulting solid phase and without separating the resulting solid phase from the resulting liquid phase.
4977080 CHEMILUMINESCENT METHODS AND A KIT THEREFOR INVOLVING A BETA-LACTAM
was characterized. Monocyte cytotoxicity inducing factor was found to be stable at pH 2 for one hour, unlike interferon-gamma, and was found to be more heat stable as well. Moreover, treatment of MCF with antisera to interferons gamma, alpha, or a combination of gamma and alpha failed to neutralize its biologic activity. MCF binds to Matrex Gel Red A. MCF eluted from this dye-ligand was found to have an apparent molecular weight of I 1,500 Daltons by gel filtration and 14,700 Daltons by SDSpolyacrylamide gel electrophoresis. MCF produced by hybridized Sezary cells appear to be neither interferon-gamma nor an altered molecular form of interferon-gamma, yet is a potent inducer of human monocyte cytotoxicity.
4977247 Dean Milbrath assigned to Minnesota Mining and Manufacturing Co Methods for the detection and measurement of compounds containing beta-lactam rings by chemiluminescence of the compounds in the presence of a chemiluminescent compound such as luminol, and for improving the sensitivity of assays using chemiluminescent reactions such as the luminol reaction utilizing compounds containing beta-lactam rings are described. A kit for conducting such chemiluminescent assays is also described.
IMMOBILIZED PROTEIN G VARIANTS AND THE USE THEREOF Stephen R Fahnestock, Timothy Lee, Marie H Wroble assigned to Genex Corporation Immobilized IgG binding proteins and the use thereof to effect affinity chromatography and separate the subclasses of IgG. Disclosed are cysteine-containing lgG binding proteins which have high binding capacity for human IgG and mouse monoclonal lgG.
4977245 METHODS AND COMPOSITIONS FOR INDUCING MONOCYTE CYTOTOXICITY C Michae Jones assigned to Board of Regents The University of Texas System The present disclosure relates to a new lymphokine molecule, referred to as Monocyte Cytotoxicity Inducing Factor (MCF), and its use as in cancer and other types of therapy. The disclosure further relates to the development of novel Sezary cell hybridomas which secrete MCF and thereby provide a ready source for MCF isolation and purification. Sezary'is Syndrome i a leukemic proliferation o f O K T 4 + lymphocytes. Sezary cells were isolated by differential centrifugation and fused to CEM.8aza.rC, an HGPRTase lacking clone of CEM. The hybrid cells were studied for their ability to produce soluble mediators of human monocyte cytotoxicity. The product of a single clone, FtF3, which bore the surface phenotype of Sezary cells,
4978608 DNA DETECTION
SYSTEM
Viola T Kung, Peter A Nagainis assigned to Molecular Devices Corporation Picogram amounts of DNA can be detected in a sample by the use of high affinity single-stranded DNA binding proteins. The assay is applicable not only to pure DNA samples but also to samples containing significant amounts of protein.
4978610 METHOD OF ASSAY EMPLOYING A MAGNETIC ELECTRODE Gordon C Forrest, Simon Rattle, Grenville Robinson, Hugh A O Hill, East Horsley, Surrey KT24 5DP, United Kingdom