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4981788 Novel acetylpolyamine amidohydrolase
300
PATENT ABSTRACTS
Combination of angiotensin converting enzyme inhibitors with potassium channel modulators and use thereof in pharmaceuticals. The invention relates to combinations of angiotensin converting enzyme inhibitors with potassium channel modulators, processes for their preparation and use thereof as medicaments.
4978470 DETERGENT COMPOSITION FOR CLOTHING: CONTAINING CELLULASE ENZYME WITH N O N - D E G R A D I N G I N D E X O F 500
OR M O R E Akira Suzuki, Susumu Ito, Kikuhik Okamoto, Eiichi Hoshino, Michio Yokosuka, Moriyasu Murata, Utsunomiya, Japan assigned to Kao Corporation A novel detergent composition for clothing comprises a cellulase having a non-degrading index of the following equation of not less than 500 See Patent for Mathematical Equation The composition can not only remove the dirt in clothing articles without damaging them but also show the effect of mitigating the roughness of cloth to the touch and the effect of preventing decoloration.
group, so that the enzyme cleaves the enzymecleavable group from the dioxetane to form a negatively charged substituent bonded to the dioxetane, the negatively charged substituent causing the dioxetane to decompose to form a luminescent substance that includes group Y of said dioxetane.
4978619 E N Z Y M E I M M O B I L I Z A T I O N BY ENTRAPMENT IN A POLYMER GEL MATRIX Shigeru Kajiwara, Hidekatsu Maeda, Hideo Suzuki, Ibaraki, Japan assigned to Agency of Industrial Science & Technology; Ministry of International Trade & Indust An immobilized enzyme having an enzyme entrapped in gaps formed in a macromolecular gel matrix is produced by dispersing the enzyme in the form of a fine powder in an organic solvent having dissolved therein a polymerizable monomer, polymerizing the monomer thereby giving rise to a gel matrix, and displacing the organic solvent in the gel matrix with an aqueous solvent.
4978632 METHOD OF PRE-TREATING SAMPLES IN PEROXIDASECATALYZED ENZYME ASSAYS
4978614 METHOD OF DETECTING A SUBSTANCE USING ENZYMATICALLY-INDUCED DECOMPOSITION OF DIOXETANES Irena Y Bronstein assigned to Tropix Inc In an assay method in which a member of a specific binding pair is detected by means of an optically detectable reaction, the improvement wherein the optically detectable reaction includes the reaction, with an enzyme, of a dioxetane having the formula See Patent for Chemical Structure where T is a cycloalkyl or polycycloalkyl group bonded to the 4-membered ring portion of the dioxetane by a spiro linkage; Y is a fluorescent chromophore; X is hydrogen, alkyl, aryl, aralkyl, alkaryl, heteroalkyl, heteroaryl, cycloalkyl, cycloheteroalkyl, or enzymecleavable group; and Z is hydrogen or an enzyme-cleavable group, provided that at least one of X or Z must be an enzyme-cleavable
Patrick A Mach, Jeffrey A Thompson, Richard S Creager, Cheri W Fink assigned to Kallestad Diagnostics Inc A method for reducing the occurrence of falsely elevated results in a peroxidase-catalyzed, enzyme assay is described. Interference in an assay caused by blood or bood products in a clinical specimen is eliminated or reduced by reacting the specimen with an oxidizing agent such as sodium hypochlorite, hydrogen peroxide or sodium meta-periodate.
4981788 NOVEL ACETYLPOLYAMINE AMIDOHYDROLASE Takayuki Uwajima, Kinya Fujishiro, Mayumi Ando, Machida, Japan assigned to Kyowa Hakko Kogyo Co Ltd A novel acetylpolyamine amidohydrolase is described. The enzyme specifically hydrolyzes
PATENT ABSTRACTS the amino bond in acetylputrescine, acetylcadaverine, acetylspermidine and acetylspermine with strong substrate affinity. The enzyme is preferably produced by culturing a microorganism belonging to the genus Mycoplana, and is used in the quantitative determination of polyamine contained in a living body sample, which is useful for cancer diagnosis.
4981789 ONE-STEP ENZYMATIC CONVERSION OF CEPHALOSPORIN C AND D E R I V A T I V E S T O 7AMINOCEPHALOSPORANIC ACID AND DERIVATIVES Joseph Lein assigned to Merck & Co Inc A process for the one-step conversion of cephalosporin C and derivatives thereof to the corresponding 7-aminocephalosporanic acid and derivatives comprising treating said cephalosporin C and derivatives with a cephalosporin C amidase derived from Arthrobacter viscosus ATCC 53594, or from any cephalosporin C amidase producing, or potentially producing descendants thereof, or from any expression of the genetic material of said Arthrobacter viscosus ATCC 53594, or any cephalosporin C amidase producing, or potentially producing descendants thereof.
4983510 ENZYMES IMMOBILIZED ON LATEX POLYMER PARTICLES FOR USE WITH AN AMINO ACID ELECTROSENSOR Pierre F Lardinois, Paul Rouxhet, William E E Stone, Kenneth Baker, Alain E Baudichau,
301
Louvain la Neuve, Monsanto Company
Belgium
assigned
to
Enzymes such as L-methionine gamma lyase, Llysine decarboxylase, L-aspartase and Ltryptophanase are immobilized by contacting with a latex of polymer particles having a negative surface charge such that the electrophoretic mobility of the latex has a negative value of-2.0 10-8m2V- 1sec- 1 or below when measured at pH 7 in 0.01M KNO3 and a polymer solids concentration of 30 mg/1. The polymer particles also have a hydrophobic surface such that the contact angle of a 1 microliter droplet of distilled water with a plane horizontal surface formed by drying and compacting the latex particles is 70 degrees or more. The resultant-enzyme/polymer particles can be used for assaying amino acids corresponding to the specific enzyme immobilized in aqueous solutions using an electrosensor comprising a pH-sensitive electrode located in a sensing zone or chamber containing the enzyme/polymer particles as an aqueous dispersion or in water-dispersible form.
4983524 METHOD OF IMMOBILIZING ENZYMES ON A SUPPORT WITH IRIDOID AGLYCONE CROSSLINKING AGENTS Shigeak Fujikawa, Kunimasa Koga, Tomoko Yokota, Osaka, Japan assigned to Suntory Limited An enzyme is immobilized by contacting the enzyme with a support in a solution containing an iridoid aglycone cross-linking agent. The crosslinking agent may be genipin or an aglycone or an iridoid glycoside such as geniposide, gardenoside or geniposide acid.
PATENT ABSTRACTS
Combination of angiotensin converting enzyme inhibitors with potassium channel modulators and use thereof in pharmaceuticals. The invention relates to combinations of angiotensin converting enzyme inhibitors with potassium channel modulators, processes for their preparation and use thereof as medicaments.
4978470 DETERGENT COMPOSITION FOR CLOTHING: CONTAINING CELLULASE ENZYME WITH N O N - D E G R A D I N G I N D E X O F 500
OR M O R E Akira Suzuki, Susumu Ito, Kikuhik Okamoto, Eiichi Hoshino, Michio Yokosuka, Moriyasu Murata, Utsunomiya, Japan assigned to Kao Corporation A novel detergent composition for clothing comprises a cellulase having a non-degrading index of the following equation of not less than 500 See Patent for Mathematical Equation The composition can not only remove the dirt in clothing articles without damaging them but also show the effect of mitigating the roughness of cloth to the touch and the effect of preventing decoloration.
group, so that the enzyme cleaves the enzymecleavable group from the dioxetane to form a negatively charged substituent bonded to the dioxetane, the negatively charged substituent causing the dioxetane to decompose to form a luminescent substance that includes group Y of said dioxetane.
4978619 E N Z Y M E I M M O B I L I Z A T I O N BY ENTRAPMENT IN A POLYMER GEL MATRIX Shigeru Kajiwara, Hidekatsu Maeda, Hideo Suzuki, Ibaraki, Japan assigned to Agency of Industrial Science & Technology; Ministry of International Trade & Indust An immobilized enzyme having an enzyme entrapped in gaps formed in a macromolecular gel matrix is produced by dispersing the enzyme in the form of a fine powder in an organic solvent having dissolved therein a polymerizable monomer, polymerizing the monomer thereby giving rise to a gel matrix, and displacing the organic solvent in the gel matrix with an aqueous solvent.
4978632 METHOD OF PRE-TREATING SAMPLES IN PEROXIDASECATALYZED ENZYME ASSAYS
4978614 METHOD OF DETECTING A SUBSTANCE USING ENZYMATICALLY-INDUCED DECOMPOSITION OF DIOXETANES Irena Y Bronstein assigned to Tropix Inc In an assay method in which a member of a specific binding pair is detected by means of an optically detectable reaction, the improvement wherein the optically detectable reaction includes the reaction, with an enzyme, of a dioxetane having the formula See Patent for Chemical Structure where T is a cycloalkyl or polycycloalkyl group bonded to the 4-membered ring portion of the dioxetane by a spiro linkage; Y is a fluorescent chromophore; X is hydrogen, alkyl, aryl, aralkyl, alkaryl, heteroalkyl, heteroaryl, cycloalkyl, cycloheteroalkyl, or enzymecleavable group; and Z is hydrogen or an enzyme-cleavable group, provided that at least one of X or Z must be an enzyme-cleavable
Patrick A Mach, Jeffrey A Thompson, Richard S Creager, Cheri W Fink assigned to Kallestad Diagnostics Inc A method for reducing the occurrence of falsely elevated results in a peroxidase-catalyzed, enzyme assay is described. Interference in an assay caused by blood or bood products in a clinical specimen is eliminated or reduced by reacting the specimen with an oxidizing agent such as sodium hypochlorite, hydrogen peroxide or sodium meta-periodate.
4981788 NOVEL ACETYLPOLYAMINE AMIDOHYDROLASE Takayuki Uwajima, Kinya Fujishiro, Mayumi Ando, Machida, Japan assigned to Kyowa Hakko Kogyo Co Ltd A novel acetylpolyamine amidohydrolase is described. The enzyme specifically hydrolyzes
PATENT ABSTRACTS the amino bond in acetylputrescine, acetylcadaverine, acetylspermidine and acetylspermine with strong substrate affinity. The enzyme is preferably produced by culturing a microorganism belonging to the genus Mycoplana, and is used in the quantitative determination of polyamine contained in a living body sample, which is useful for cancer diagnosis.
4981789 ONE-STEP ENZYMATIC CONVERSION OF CEPHALOSPORIN C AND D E R I V A T I V E S T O 7AMINOCEPHALOSPORANIC ACID AND DERIVATIVES Joseph Lein assigned to Merck & Co Inc A process for the one-step conversion of cephalosporin C and derivatives thereof to the corresponding 7-aminocephalosporanic acid and derivatives comprising treating said cephalosporin C and derivatives with a cephalosporin C amidase derived from Arthrobacter viscosus ATCC 53594, or from any cephalosporin C amidase producing, or potentially producing descendants thereof, or from any expression of the genetic material of said Arthrobacter viscosus ATCC 53594, or any cephalosporin C amidase producing, or potentially producing descendants thereof.
4983510 ENZYMES IMMOBILIZED ON LATEX POLYMER PARTICLES FOR USE WITH AN AMINO ACID ELECTROSENSOR Pierre F Lardinois, Paul Rouxhet, William E E Stone, Kenneth Baker, Alain E Baudichau,
301
Louvain la Neuve, Monsanto Company
Belgium
assigned
to
Enzymes such as L-methionine gamma lyase, Llysine decarboxylase, L-aspartase and Ltryptophanase are immobilized by contacting with a latex of polymer particles having a negative surface charge such that the electrophoretic mobility of the latex has a negative value of-2.0 10-8m2V- 1sec- 1 or below when measured at pH 7 in 0.01M KNO3 and a polymer solids concentration of 30 mg/1. The polymer particles also have a hydrophobic surface such that the contact angle of a 1 microliter droplet of distilled water with a plane horizontal surface formed by drying and compacting the latex particles is 70 degrees or more. The resultant-enzyme/polymer particles can be used for assaying amino acids corresponding to the specific enzyme immobilized in aqueous solutions using an electrosensor comprising a pH-sensitive electrode located in a sensing zone or chamber containing the enzyme/polymer particles as an aqueous dispersion or in water-dispersible form.
4983524 METHOD OF IMMOBILIZING ENZYMES ON A SUPPORT WITH IRIDOID AGLYCONE CROSSLINKING AGENTS Shigeak Fujikawa, Kunimasa Koga, Tomoko Yokota, Osaka, Japan assigned to Suntory Limited An enzyme is immobilized by contacting the enzyme with a support in a solution containing an iridoid aglycone cross-linking agent. The crosslinking agent may be genipin or an aglycone or an iridoid glycoside such as geniposide, gardenoside or geniposide acid.