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4990442 Assay for an analyte on a solid porous support
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PATENT ABSTRACTS 4990441 PROCESS FOR SEPARATING 2-KETO-L-GULONIC ACID FROM A FERMENTED MEDIUM Jean-Pierre Barthole, Jean Filippi, Aurelia Jaeger-Seddik, Isidore Le Fur, Jean-Yves Pommier, Elbeuf, France assigned to Rhone-Poulenc Sante 2-Keto-L-gulonic acid is separated from a fermented medium containing the calcium salt of 2keto-L-gulonic acid, by carrying out the following operations: separation of insolubles: removal of inorganic cations; and separation of the 2-keto-L-gulonic acid.
vention also provides hapten-protein conjugates of the general formula: See Patent for Chemical Structure (III) wherein Hap, R 1 and R2 have the above-given meanings and E-NH is the residue of a protein bound via an epsilon-amino group of a lysine residue.
4990444 GAMMA-GLUTAMYL TRANSPEPTIDASE, ITS PREPARATION AND ITS USE Werner Aretz, Klaus Sauber, Kfederal Republic Of Germana assigned to Hoechst Aktiengesellschaft
A S S A Y F O R AN A N A L Y T E O N A SOLID POROUS SUPPORT
It is possible with the aid of a gamma-glutamyl transpeptidase, which can be prepared by fermentation, to hydrolyze adipinyl- or glutarylmonoamino compounds, in particular alphaketo-adipinylor glutaryl-7aminocephalosporani c acid.
Campo G B Del, Milan, Italy assigned to Chemetron
4990445
4990442
An assay for an analyte wherein sample is applied to a support capable of binding proteins by essentially only hydrogen bonding and fixed on the support. Analyte may be determined on the support by use of a suitable tracer. A preferred support is amphiphilic cellulose acetate. In an immunoassay, it is possible to determine analyte without use of a supported ( capture ) antibody.
4990443 HAPTEN-PROTEIN CONJUGATES AND METHODS OF USE IN IMMUNOASSAYS Erasmus Huber, Christian Klein, Gunter Pappert, Klaus Hallermayer, Garching. Federal Republic Of Germany assigned to Boehringer Mannhcim GmbH The present invention provides hapten derivatives of the general formula: See Patent for Chemical Structure (I) wherein Hap is a residue formed from a hapten carrying a keto or aldehyde group by splitting off an oxo group and RI and R2, which can be the same or different. are alkyl radicals containing up to 7 carbon atoms and one of the symbols R1 and R2 can also represent a hydrogen atom. The present in-
STABLE REAGENT AND KINETIC ASSAY FOR ALPHA-AMYLASE Sandra M Poudrier, Mark T Oyen assigned to Beckman Instruments Inc A stable reagent and kinetic assay for alphaamylase is provided. The reagent comprises a substrate for the alpha-amylase and various enzymes and cofactors necessary to produce NADH as the end-product of a series of four reactions. The substrate, enzymes, and cofactors are provided in sufficient excess that the alphaamylase from the test sample is the rate-limiting factor. The concentration of alpha-amylase in a test sample is determined by measuring the rate of increase in absorbance caused by the production of NADH. The use of the novel cofactor fructose-l, 6-diphosphate has been found to impart improved stability to the reagent. 4990447 PROCESS FOR THE PURIFICATION OF SERUM ALBUMIN Boudewijn W Konig. Michiel Hamers, Laken Cornelis van der, Amsterdam, Netherlands assigned to Gist-Brocades NV
PATENT ABSTRACTS 4990441 PROCESS FOR SEPARATING 2-KETO-L-GULONIC ACID FROM A FERMENTED MEDIUM Jean-Pierre Barthole, Jean Filippi, Aurelia Jaeger-Seddik, Isidore Le Fur, Jean-Yves Pommier, Elbeuf, France assigned to Rhone-Poulenc Sante 2-Keto-L-gulonic acid is separated from a fermented medium containing the calcium salt of 2keto-L-gulonic acid, by carrying out the following operations: separation of insolubles: removal of inorganic cations; and separation of the 2-keto-L-gulonic acid.
vention also provides hapten-protein conjugates of the general formula: See Patent for Chemical Structure (III) wherein Hap, R 1 and R2 have the above-given meanings and E-NH is the residue of a protein bound via an epsilon-amino group of a lysine residue.
4990444 GAMMA-GLUTAMYL TRANSPEPTIDASE, ITS PREPARATION AND ITS USE Werner Aretz, Klaus Sauber, Kfederal Republic Of Germana assigned to Hoechst Aktiengesellschaft
A S S A Y F O R AN A N A L Y T E O N A SOLID POROUS SUPPORT
It is possible with the aid of a gamma-glutamyl transpeptidase, which can be prepared by fermentation, to hydrolyze adipinyl- or glutarylmonoamino compounds, in particular alphaketo-adipinylor glutaryl-7aminocephalosporani c acid.
Campo G B Del, Milan, Italy assigned to Chemetron
4990445
4990442
An assay for an analyte wherein sample is applied to a support capable of binding proteins by essentially only hydrogen bonding and fixed on the support. Analyte may be determined on the support by use of a suitable tracer. A preferred support is amphiphilic cellulose acetate. In an immunoassay, it is possible to determine analyte without use of a supported ( capture ) antibody.
4990443 HAPTEN-PROTEIN CONJUGATES AND METHODS OF USE IN IMMUNOASSAYS Erasmus Huber, Christian Klein, Gunter Pappert, Klaus Hallermayer, Garching. Federal Republic Of Germany assigned to Boehringer Mannhcim GmbH The present invention provides hapten derivatives of the general formula: See Patent for Chemical Structure (I) wherein Hap is a residue formed from a hapten carrying a keto or aldehyde group by splitting off an oxo group and RI and R2, which can be the same or different. are alkyl radicals containing up to 7 carbon atoms and one of the symbols R1 and R2 can also represent a hydrogen atom. The present in-
STABLE REAGENT AND KINETIC ASSAY FOR ALPHA-AMYLASE Sandra M Poudrier, Mark T Oyen assigned to Beckman Instruments Inc A stable reagent and kinetic assay for alphaamylase is provided. The reagent comprises a substrate for the alpha-amylase and various enzymes and cofactors necessary to produce NADH as the end-product of a series of four reactions. The substrate, enzymes, and cofactors are provided in sufficient excess that the alphaamylase from the test sample is the rate-limiting factor. The concentration of alpha-amylase in a test sample is determined by measuring the rate of increase in absorbance caused by the production of NADH. The use of the novel cofactor fructose-l, 6-diphosphate has been found to impart improved stability to the reagent. 4990447 PROCESS FOR THE PURIFICATION OF SERUM ALBUMIN Boudewijn W Konig. Michiel Hamers, Laken Cornelis van der, Amsterdam, Netherlands assigned to Gist-Brocades NV