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4994370 DNA amplification technique
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PATENT ABSTRACTS
pharmaceutically acceptable salt thereof, is useful for the treatment of calcium metabolic disorders, cardiac disease and ulcers, and for the improvement of cerebral circulation.
4992537 CDNA ENCODING 92-KDA TYPE IV COLLAGENASE Gregory I Goldberg, Arthur Eisen assigned to Washington University A novel 92-kDa type IV collagenase has been purifed to homogeneity from SV-40 transformed fetal lung fibroblasts, its primary structure determined and characterized and a cDNA clone representing the full size protein has been developed.
4994~9 T-CELL AC~VATION GENE
RELATED
Alan Krensky, Mark Davis, Thomas SchaU, Jan Jongstra assigned to The Board of Trustees of the Leland Stanford Jr Univ Nucleic acids and peptides are provided which can be used for detecting the status of functional T-lymphocytes as to stimulation and the time of stimulation. The nucleic acids and peptides may be provided by cloning and expression using recombinant techniques. These diagnoses may be used to determine whether T-cells are functional and the degree to which a T-cell population has been stimulated.
4994370 49943~ AMPLIFICATION METHOD FOR POLYNUCLEOTIDE ASSAYS Thomas C Goodman, Marti Becker, Edwin Ullman, Samue Rose assigned to Syntex (U S A ) Inc A method is disclosed for producing multiple copies of a primary polynucleotide sequence located at the 3' terminus of a polynucleotide. The method comprises (a) forming in the presence of nucleoside triphosphates and templatedependent polynucleotide polymerase an extension of a primary polynucleotide sequence hybridized with a template sequence of a single stranded pattern polynucleotide comprising two or more template sequences each containing one or more site specific cleavage sequences, (b) cleaving into fragments said extension at cleavable polynucleotide sequences in the presence of means for specifically cleaving said cleavable polynucleotide sequences when said extension is hybridized with said site specific cleavage sequences, (c) dissociating said fragments, (d) hybridizing said fragments with single stranded pattern polynucleotide, and repeating steps (a)-(d). Steps (a)-(d) may be conducted simultaneously or wholly or partially sequentially. The method may be applied in the detection of a polynucleotide analyte in a sample suspected of containing such analyte to facilitate such detection. Also disclosed are compositions for conducting the method of the invention.
DNA AMPLIFICATION TECHNIQUE Jonatha Silver, Vijaya Keerikatte assigned to The United States of America as represented by the Department of Health and Human Services A modification of the polymerase chain reaction (PCR) technique is described. The method allows the amplification of regions of DNA flanking a single region of known sequence, in contrast to standard PCR which requires two regions of known sequence at opposite ends of the fragment to be amplified. Various advantages of the new method are described.
4994371 DNA PREPARATION OF CHRISTMAS FACTOR AND USE OF DNA SEQUENCES Earl W Davie, Kotoku Kurachi There is disclosed an isolated DNA sequence and the amino acid sequence for human factor IX. The isolated DNA sequence and its flanking sequences are useful for determining mutations, deletions or other modifications in genetic sequences expressing normal factor IX or modifications thereof.
PATENT ABSTRACTS
pharmaceutically acceptable salt thereof, is useful for the treatment of calcium metabolic disorders, cardiac disease and ulcers, and for the improvement of cerebral circulation.
4992537 CDNA ENCODING 92-KDA TYPE IV COLLAGENASE Gregory I Goldberg, Arthur Eisen assigned to Washington University A novel 92-kDa type IV collagenase has been purifed to homogeneity from SV-40 transformed fetal lung fibroblasts, its primary structure determined and characterized and a cDNA clone representing the full size protein has been developed.
4994~9 T-CELL AC~VATION GENE
RELATED
Alan Krensky, Mark Davis, Thomas SchaU, Jan Jongstra assigned to The Board of Trustees of the Leland Stanford Jr Univ Nucleic acids and peptides are provided which can be used for detecting the status of functional T-lymphocytes as to stimulation and the time of stimulation. The nucleic acids and peptides may be provided by cloning and expression using recombinant techniques. These diagnoses may be used to determine whether T-cells are functional and the degree to which a T-cell population has been stimulated.
4994370 49943~ AMPLIFICATION METHOD FOR POLYNUCLEOTIDE ASSAYS Thomas C Goodman, Marti Becker, Edwin Ullman, Samue Rose assigned to Syntex (U S A ) Inc A method is disclosed for producing multiple copies of a primary polynucleotide sequence located at the 3' terminus of a polynucleotide. The method comprises (a) forming in the presence of nucleoside triphosphates and templatedependent polynucleotide polymerase an extension of a primary polynucleotide sequence hybridized with a template sequence of a single stranded pattern polynucleotide comprising two or more template sequences each containing one or more site specific cleavage sequences, (b) cleaving into fragments said extension at cleavable polynucleotide sequences in the presence of means for specifically cleaving said cleavable polynucleotide sequences when said extension is hybridized with said site specific cleavage sequences, (c) dissociating said fragments, (d) hybridizing said fragments with single stranded pattern polynucleotide, and repeating steps (a)-(d). Steps (a)-(d) may be conducted simultaneously or wholly or partially sequentially. The method may be applied in the detection of a polynucleotide analyte in a sample suspected of containing such analyte to facilitate such detection. Also disclosed are compositions for conducting the method of the invention.
DNA AMPLIFICATION TECHNIQUE Jonatha Silver, Vijaya Keerikatte assigned to The United States of America as represented by the Department of Health and Human Services A modification of the polymerase chain reaction (PCR) technique is described. The method allows the amplification of regions of DNA flanking a single region of known sequence, in contrast to standard PCR which requires two regions of known sequence at opposite ends of the fragment to be amplified. Various advantages of the new method are described.
4994371 DNA PREPARATION OF CHRISTMAS FACTOR AND USE OF DNA SEQUENCES Earl W Davie, Kotoku Kurachi There is disclosed an isolated DNA sequence and the amino acid sequence for human factor IX. The isolated DNA sequence and its flanking sequences are useful for determining mutations, deletions or other modifications in genetic sequences expressing normal factor IX or modifications thereof.