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4997767 Yeast shuttle vector
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PATENT ABSTRACTS 4997764
4997929
TRANSFORMATION OF HUMAN B-LYMPOCYTES WITH EPSTEIN BARR VIRUS AND C-MYC CONTAINING VECTORS
PURIFIED CILIARY NEUROTROPHIC FACTOR
Favera Ricardo Dalla assigned to New York University Disclosed herein is a method for transforming human B-cells by infecting them with Epstein Barr virus and transfecting the Epstein Barr virus infected cells with an activated human cmyc gene. The transformed cells are useful for producing human monoclonal antibodies.
4997766 POLY-KRINGLE PLASMINOGEN ACTIVATOR
Franklin D Collins, Leu-Fen Lin assigned to Synergen Inc A ciliary neurotrophic factor (CNTF), particularly sciatic nerve CNTF(SN-CNFT) is claimed. The SN-CNTF described herein is a single protein species and has a specific activity that increased to greater than 25,000-tbld from crude extract. Amino acid data for this SNCNTF is also provided. In addition, methods for using this data for providing SN-CNTF probes and for screening eDNA and genomic libraries are also provided. Recombinant-DNA methods for the production of SN-CNTF are described. Nucleic acid sequences encoding rabbit and human CNTF are provided. A recombinant expression system is provided for producing biologically active CNTF.
49979~ Paul P Hung, Narender K Kalyan, Shaw-guang L Lee assigned to American Home Products Corporation Hybrid, third generation, plasminogen activators containing plural, heterologous polypeptide kringles prepared by recombinant DNA techniques as well as the genes coding for the activators. Vectors containing those genes and a method for using the plasminogen activators as thrombolytic agents, are disclosed.
4997767 YEAST SHUTTLE
CLONING OF COMPLEMENTARY DNA ENCODING MAIZE NITRITE REDUCTASE Kristine N Lahners, Steven J Rothstein assigned to Ciba-Geigy Corporation Maize eDNA coding for nitrite reductase is cloned, using a spinach nitrite reductase eDNA as a heterologous probe, and is characterized. A method is provided to use the cloned maize nitrite reductase eDNA to determine the number of nitrite reductase genes per maize genome and to stud)' nitrite reductase mRNA regulation in maize.
VECTOR 4997932
Chikateru Nozaki, Fukusaburo Hamada, Nobuya Ohtomo, Kumamoto, Japan assigned to Juridical Foundation The Chemo-SeroTherapeutic Research Institute Shuttle vectors including a DNA sequence of the yeast Saccharomyces cerevisiae including ars 1.2 micron ori and a marker gene for transformed yeast permitting synthesis ofleucine by the transformant; adjacent to said ars I a DNA sequence of Escherichia coil which is either in EcoRlPvulI or EcoRI-Tthlll-I fragment of plasmid pBR322; and adjacent to the yeast marker gene the expression control region of the repressible acid phosphatase gene of yeast.
METHOD AND KIT FOR PURIFYING NUCLEIC ACIDS Melissa A Reardon, Lisa S Klein assigned to Boehringer Mannheim Corporation The invention teaches a method and kit for purifying nucleic acid, such as DNA. from a sample, such as lysed cell or tissue sample. A sample is applied to an anionic exchange matrix column uniformly distributing the sample therein. The column bed is then washed with a weak ionic salt solution which is then removed. The anionic exchange material is optionally
PATENT ABSTRACTS 4997764
4997929
TRANSFORMATION OF HUMAN B-LYMPOCYTES WITH EPSTEIN BARR VIRUS AND C-MYC CONTAINING VECTORS
PURIFIED CILIARY NEUROTROPHIC FACTOR
Favera Ricardo Dalla assigned to New York University Disclosed herein is a method for transforming human B-cells by infecting them with Epstein Barr virus and transfecting the Epstein Barr virus infected cells with an activated human cmyc gene. The transformed cells are useful for producing human monoclonal antibodies.
4997766 POLY-KRINGLE PLASMINOGEN ACTIVATOR
Franklin D Collins, Leu-Fen Lin assigned to Synergen Inc A ciliary neurotrophic factor (CNTF), particularly sciatic nerve CNTF(SN-CNFT) is claimed. The SN-CNTF described herein is a single protein species and has a specific activity that increased to greater than 25,000-tbld from crude extract. Amino acid data for this SNCNTF is also provided. In addition, methods for using this data for providing SN-CNTF probes and for screening eDNA and genomic libraries are also provided. Recombinant-DNA methods for the production of SN-CNTF are described. Nucleic acid sequences encoding rabbit and human CNTF are provided. A recombinant expression system is provided for producing biologically active CNTF.
49979~ Paul P Hung, Narender K Kalyan, Shaw-guang L Lee assigned to American Home Products Corporation Hybrid, third generation, plasminogen activators containing plural, heterologous polypeptide kringles prepared by recombinant DNA techniques as well as the genes coding for the activators. Vectors containing those genes and a method for using the plasminogen activators as thrombolytic agents, are disclosed.
4997767 YEAST SHUTTLE
CLONING OF COMPLEMENTARY DNA ENCODING MAIZE NITRITE REDUCTASE Kristine N Lahners, Steven J Rothstein assigned to Ciba-Geigy Corporation Maize eDNA coding for nitrite reductase is cloned, using a spinach nitrite reductase eDNA as a heterologous probe, and is characterized. A method is provided to use the cloned maize nitrite reductase eDNA to determine the number of nitrite reductase genes per maize genome and to stud)' nitrite reductase mRNA regulation in maize.
VECTOR 4997932
Chikateru Nozaki, Fukusaburo Hamada, Nobuya Ohtomo, Kumamoto, Japan assigned to Juridical Foundation The Chemo-SeroTherapeutic Research Institute Shuttle vectors including a DNA sequence of the yeast Saccharomyces cerevisiae including ars 1.2 micron ori and a marker gene for transformed yeast permitting synthesis ofleucine by the transformant; adjacent to said ars I a DNA sequence of Escherichia coil which is either in EcoRlPvulI or EcoRI-Tthlll-I fragment of plasmid pBR322; and adjacent to the yeast marker gene the expression control region of the repressible acid phosphatase gene of yeast.
METHOD AND KIT FOR PURIFYING NUCLEIC ACIDS Melissa A Reardon, Lisa S Klein assigned to Boehringer Mannheim Corporation The invention teaches a method and kit for purifying nucleic acid, such as DNA. from a sample, such as lysed cell or tissue sample. A sample is applied to an anionic exchange matrix column uniformly distributing the sample therein. The column bed is then washed with a weak ionic salt solution which is then removed. The anionic exchange material is optionally