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5003059 Determining DNA sequences by mass spectrometry
PATENT ABSTRACTS A mutant Bacillus sphaericus strain ATC No. 53969 which has the property of sulfur removal and sulfur metabolism by selective cleavage of CS bonds in organic carbonaceous materials.
5002891 MULTIFUNCTIONAL PLASMID VECTORS FROM ACTINOMADURA AND ESCHERICHIA COLI Jerald S Feitelson assigned to Cyanamid Company
American
The present invention discloses multifunctional recombinant DNA plasmid cloning vectors able to replicate and express dominant drug resistance markers in Escherichia coli, Streptomyces, and Actinomadura.
449
Chemical Structure The product, which is a polymer having repeating units comprising diazolinked luminol and hydroxyindole, is referred to as diazoluminomelanin (DALM), since one of the precursors to this product, 3-amino-Ltyrosine (3AT) is closely related to the biological substrates which are converted into melanin. Also provided is a method for preparing DALM which comprises reacting 3AT with an alkali metal nitrite, and reacting the resulting diazonium salt with luminol. DALM can be used for chemiluminescent immunoassays for biological and chemical agents; in radiofrequency and ionizing radiation dosimeters; and for RNA/DNA hybridiTation assays for viruses and genetic detection.
5003058 DNA CODING FOR ANTIGEN PROTEIN OF RINDERPEST VIRUS
5003045 MODIFIED 7S LEGUME SEED STORAGE PROTEINS Leslie Hoffman assigned to Lubrizol Genetics Inc The present invention discloses plant cells which contain modified 7S legume seed storage protein. Modification of 7S seed storage proteins which are expressible in plant cells and transformation of such genes into plant cells is also taught. Furthermore, methods and DNA molecules useful for producing plant cells containing modified 7S seed storage proteins are also disclosed. The invention is exemplified by insertion of an oligonucleotide encoding 15 amino acid residues, including 6 methionines, into a Phaseolus vulgaris phaseolin gene, thereby tripling its content of sulfur-containing amino acids.
5003050 DIAZOLUMINOMELANIN AND A METHOD FOR PREPARING SAME Johnathan L Kiel, Gerald J O'Brien assigned to The United States of America as represented by the Secretary of the Air Force A water-soluble luminescent compound having repeating units of the formula: See Patent for
Kazuya Yamanouchi, Yasuhiro Yoshikawa. Masanobu Sugimoto, Fuchu, Japan assigned to Toa Nenryo Kogyo Kabushiki Kaisha A DNA coding for an entire or a part of an antigen protein of the rinderpest virus; a protein for the production of the DNA comprising the steps of preparing an m R N A from the rinderpest virus, preparing a cDNA library from the mRNA, selecting a cDNA coding for the target protein from the cDNA library, and cloning the selected cDNA in a cloning vector; and a protein for the production of the antigen protein of the rinderpest virus comprising the steps of transletting a vector containing the DNA coding for the target protein into animal cells, culturing the animal cells to produce the antigen protein, and recovering the target protein from the cell culture. 5003059 DETERMINING DNA SEQUENCES BY M A S S S P E C T R O M E T R Y Thomas M Brennan assigned to Genomyx Inc This invention relates to the methods, apparatus, reagents and mixtures of reagents for sequencing natural or recombinant DNA and other polynucleotides. In particular, this invention relates to a method for sequencing polynueleotides based on mass spectrometry to determine which of the four bases (adenine,
450
PATENT ABSTRACTS
guanine, cytosine or thymine) is a component of the terminal nucleotide. In particular, the present invention relates to identifying the individual nucleotides by the mass of stable nuclide markers contained within either the dideoxynucleotides, the DNA primer, or the deoxynucleotide added to the primer. This invention is particularly useful in identifying specific DNA sequences in very small quantities in biological products produced by fermentation or other genetic engineering techniques. The invention is therefore useful in evaluating safety and other health concerns related to the presence of DNA in products resulting from genetic engineering techniques.
A method of measuring chain breakage in the DNA of a eucaryotic cell is disclosed. This method includes (a) contacting the cell with a stripping solution that lyses and solubilizes the cell without denaturing its DNA, thereby forming a nuclcoid having a halo, (b) measuring the width of the halo. and (c) determining the number of chain breaks from the measured width. The halo includes at least one loop of undenatured DNA, and has a width related to the number of DNA chain breaks in the loop. The cell to be examined may be adhered to a support prior to its contact with the stripping solution. The number of DNA chain breaks can be determined by compairing the measured width of the nucleoid halo with a reference value.
5006252 PURIFIED PROTEASE
NEXIN
Randy W Scott, Joffre Baker assigned to Invitron; University of Kans The gene encoding protease nexin I (PN-I) is cloned and expressed to provide practical quantities of PN- 1 for diagnostic and therapeutic use. PN-I is a serine protcase inhibitor useful in controlling conditions mediated by proteolytic activity.
5006336 NOVEL COLEOPTERAN-ACTIVE BACILLUS THURINGIENSIS ISOLATE Jewel Payne assigned to Mycogen Corporation The subject invention concerns a novel and useful insecticide with activity against insect pests of the order Coleopters. Pests in the order Coleoptera do heavy damage to crops, e.g.. corn and potatoes. The insecticide of the subject invention is a novel B. thuringiensis microbe referred to as B.t. PSI22D3, or mutants thereof. The spores or crystals of this microbe are useful to control coleopteran pests in various environments.
5O0646O METHOD FOR MEASURING DNA DAMAGE IN SINGLE CELLS Charles A Thomas, Eric A Thomas assigned to Pantox Corporation
5006465 PROCESS FOR PREPARING BOVINE INTERLEUKIN-2 Dirk M Anderson, Paul E Baker, Michael Cantre]l, Douglas Cerretti. David Cosman, Steven D Gimpel. Kenneth H Grabstein, All D Larsen, Kate McKereghan A process for preparing bovine interleukin-2 (blL-2) by culturing a microbial host transformed by a vector containing a DNA sequence encoding blL-2 under conditions suitable for the expression of blL-2 and recovering blL-2.
5006469 VECTORS AND HOST CELLS EXPRESSING PREPROMOTILIN AND MOTILIN-ASSOCIATED PEPTIDE (MAP) John P Adelman, Chris Bond, Gajanan Nilaver, Earl Zimmerman assigned to Oregon Health Sciences University eDNA encoding a precursor peptide for motilin has been retrieved from porcine gut tissue. The precursor protein contains a motilin-associated peptide (MAP) .sequence conjugated to the motilin peptide through a trypsin-like cleavage site. The MAP is useful in regulating alimentary physiology in vertebrates. The DNA provides a means to obtain the corresponding DNAs in other vertebrate species, including humans. It also makes available recombinant motilin. motilin precursor, and MAP of any desired species.
5002891 MULTIFUNCTIONAL PLASMID VECTORS FROM ACTINOMADURA AND ESCHERICHIA COLI Jerald S Feitelson assigned to Cyanamid Company
American
The present invention discloses multifunctional recombinant DNA plasmid cloning vectors able to replicate and express dominant drug resistance markers in Escherichia coli, Streptomyces, and Actinomadura.
449
Chemical Structure The product, which is a polymer having repeating units comprising diazolinked luminol and hydroxyindole, is referred to as diazoluminomelanin (DALM), since one of the precursors to this product, 3-amino-Ltyrosine (3AT) is closely related to the biological substrates which are converted into melanin. Also provided is a method for preparing DALM which comprises reacting 3AT with an alkali metal nitrite, and reacting the resulting diazonium salt with luminol. DALM can be used for chemiluminescent immunoassays for biological and chemical agents; in radiofrequency and ionizing radiation dosimeters; and for RNA/DNA hybridiTation assays for viruses and genetic detection.
5003058 DNA CODING FOR ANTIGEN PROTEIN OF RINDERPEST VIRUS
5003045 MODIFIED 7S LEGUME SEED STORAGE PROTEINS Leslie Hoffman assigned to Lubrizol Genetics Inc The present invention discloses plant cells which contain modified 7S legume seed storage protein. Modification of 7S seed storage proteins which are expressible in plant cells and transformation of such genes into plant cells is also taught. Furthermore, methods and DNA molecules useful for producing plant cells containing modified 7S seed storage proteins are also disclosed. The invention is exemplified by insertion of an oligonucleotide encoding 15 amino acid residues, including 6 methionines, into a Phaseolus vulgaris phaseolin gene, thereby tripling its content of sulfur-containing amino acids.
5003050 DIAZOLUMINOMELANIN AND A METHOD FOR PREPARING SAME Johnathan L Kiel, Gerald J O'Brien assigned to The United States of America as represented by the Secretary of the Air Force A water-soluble luminescent compound having repeating units of the formula: See Patent for
Kazuya Yamanouchi, Yasuhiro Yoshikawa. Masanobu Sugimoto, Fuchu, Japan assigned to Toa Nenryo Kogyo Kabushiki Kaisha A DNA coding for an entire or a part of an antigen protein of the rinderpest virus; a protein for the production of the DNA comprising the steps of preparing an m R N A from the rinderpest virus, preparing a cDNA library from the mRNA, selecting a cDNA coding for the target protein from the cDNA library, and cloning the selected cDNA in a cloning vector; and a protein for the production of the antigen protein of the rinderpest virus comprising the steps of transletting a vector containing the DNA coding for the target protein into animal cells, culturing the animal cells to produce the antigen protein, and recovering the target protein from the cell culture. 5003059 DETERMINING DNA SEQUENCES BY M A S S S P E C T R O M E T R Y Thomas M Brennan assigned to Genomyx Inc This invention relates to the methods, apparatus, reagents and mixtures of reagents for sequencing natural or recombinant DNA and other polynucleotides. In particular, this invention relates to a method for sequencing polynueleotides based on mass spectrometry to determine which of the four bases (adenine,
450
PATENT ABSTRACTS
guanine, cytosine or thymine) is a component of the terminal nucleotide. In particular, the present invention relates to identifying the individual nucleotides by the mass of stable nuclide markers contained within either the dideoxynucleotides, the DNA primer, or the deoxynucleotide added to the primer. This invention is particularly useful in identifying specific DNA sequences in very small quantities in biological products produced by fermentation or other genetic engineering techniques. The invention is therefore useful in evaluating safety and other health concerns related to the presence of DNA in products resulting from genetic engineering techniques.
A method of measuring chain breakage in the DNA of a eucaryotic cell is disclosed. This method includes (a) contacting the cell with a stripping solution that lyses and solubilizes the cell without denaturing its DNA, thereby forming a nuclcoid having a halo, (b) measuring the width of the halo. and (c) determining the number of chain breaks from the measured width. The halo includes at least one loop of undenatured DNA, and has a width related to the number of DNA chain breaks in the loop. The cell to be examined may be adhered to a support prior to its contact with the stripping solution. The number of DNA chain breaks can be determined by compairing the measured width of the nucleoid halo with a reference value.
5006252 PURIFIED PROTEASE
NEXIN
Randy W Scott, Joffre Baker assigned to Invitron; University of Kans The gene encoding protease nexin I (PN-I) is cloned and expressed to provide practical quantities of PN- 1 for diagnostic and therapeutic use. PN-I is a serine protcase inhibitor useful in controlling conditions mediated by proteolytic activity.
5006336 NOVEL COLEOPTERAN-ACTIVE BACILLUS THURINGIENSIS ISOLATE Jewel Payne assigned to Mycogen Corporation The subject invention concerns a novel and useful insecticide with activity against insect pests of the order Coleopters. Pests in the order Coleoptera do heavy damage to crops, e.g.. corn and potatoes. The insecticide of the subject invention is a novel B. thuringiensis microbe referred to as B.t. PSI22D3, or mutants thereof. The spores or crystals of this microbe are useful to control coleopteran pests in various environments.
5O0646O METHOD FOR MEASURING DNA DAMAGE IN SINGLE CELLS Charles A Thomas, Eric A Thomas assigned to Pantox Corporation
5006465 PROCESS FOR PREPARING BOVINE INTERLEUKIN-2 Dirk M Anderson, Paul E Baker, Michael Cantre]l, Douglas Cerretti. David Cosman, Steven D Gimpel. Kenneth H Grabstein, All D Larsen, Kate McKereghan A process for preparing bovine interleukin-2 (blL-2) by culturing a microbial host transformed by a vector containing a DNA sequence encoding blL-2 under conditions suitable for the expression of blL-2 and recovering blL-2.
5006469 VECTORS AND HOST CELLS EXPRESSING PREPROMOTILIN AND MOTILIN-ASSOCIATED PEPTIDE (MAP) John P Adelman, Chris Bond, Gajanan Nilaver, Earl Zimmerman assigned to Oregon Health Sciences University eDNA encoding a precursor peptide for motilin has been retrieved from porcine gut tissue. The precursor protein contains a motilin-associated peptide (MAP) .sequence conjugated to the motilin peptide through a trypsin-like cleavage site. The MAP is useful in regulating alimentary physiology in vertebrates. The DNA provides a means to obtain the corresponding DNAs in other vertebrate species, including humans. It also makes available recombinant motilin. motilin precursor, and MAP of any desired species.