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5014225 Apparatus and method for determining plant fluorescence
PATENT ABSTRACTS
5?,6
the viruses to human tissue, isolating, cultivating, and then processing the live mumps viruses. 5006336
germination is carried out in B5 or MS medium containing indolebutyric acid and gibberellic acid or MS medium containing filter sterilized Lproline and sucrose. Plantlet formation is carried out in B5 or MS medium without growth regulators. Plant and seed formation is carried out in soil or potting medium.
NOVEL COLEOPTERAN-ACTIVE BACILI,US THURINGIENSIS ISOLATE Jewel Payne assigned to Mycogen Corporation The subject invention concerns a novel and useful insecticide with activity against insect pests of the order Coleopters. Pests in the order Coleoptera do heavy damage to crops, e.g., corn and potatoes. The insecticide of the subject invention is a novel B. thuringiensis microbe referred to as B.t. PSI22D3, or mutants thereof. The spores or crystals of this microbe are useful to control coleopteran pests in various environments.
5008200 PROPAGATING MULTIPLE WHOLE FERTILE PLANTS FROM IMMATURE LEGUMINOUS
5012761 CHICKEN EGG HAVING RELATIVELY HIGH PERCENTAGE OF LONG CHAIN FATTY ACIDS AND METHOD OF REDUCING HEART RELATED D I S E A S E IN H U M A N S U S I N G SUCH EGGS Suk Y Oh Chickens are fed a composition including fish oil over a period of time. Modified eggs laid by such chickens contain substantial quantities of omega-3 polyunsaturated fatty acids. The modified eggs are fed to humans with the result that serum cholesterol, serum triglycerides and blood pressure are reduced as compared to humans eating a like number of regular eggs with a subsequent decrease in heart disease.
Jerome P Ranch, Julie Buchheim assigned to United AgriSeeds lnc Seeds of Glycine max, Glycine soja and hybrids thereof are produced by propagating embryogenic tissue containing multiple immature somatic embryos directly or serially by culturing particular cotyledonary tissue or derivation product thereof on media containing auxin selected from the group consisting of chloro-substituted phenoxyacetic acid, methyl derivative thereof, dicamba or picloram, incubating individual immature somatic embryos or embryogenic tissue containing such in a maturation stage to produce mature somatic embryos, germinating the mature somatic embryos to cause shoot tip formation, cultivating the germinated embryos to provide plantlets with 2 to 5 nodes and roots, cultivating the plantlets to provide whole fertile plants which flower and bear seeds, and recovering the seeds. Culturing to provide immature somatic embryos is carried out utilizing auxin in a concentration within the range of 2.5 to 1000 ppm effective to foster normal development of immature somatic embryos. 2,4dichlorophenoxyacetic acid is the preferred auxin. Maturation is carried out in B5 or MS medium containing indolebutyric acid and abscissic acid or activated charcoal and sucrose, and
5014225 APPARATUS AND METHOD FOR DETERMINING PLANT FLUORESCENCE William Vidaver, Peter Toivoncn. Sylvain Dube, West Vancouver, Canada assigned to Simon Fraser University An apparatus and method for determining the photosynthetic activity of a plant by determining the chlorophyll fluorescence of the plant is provided. The apparatus includes a light impermeable housing, a light to illuminate the housing, a light intensity in the housing and to adjust the light intensity controller. The light intensity may be between zero and 700 micromoles of photons per square meter per second. The monitor may be a photodiode protected by light filters which permit only light of wavelengths corresponding to plant fluorescence emission to pass to the photodiode. A computer analysis of the data obtained, corrects for Dark and Straylight signals in the housing and normalizes the data by correcting for the Fo fluoreseence. A
PATENT ABSTRACTS method of estimating Fo is provided which includes illuminating a light impermeable chamber housing a plant with light ofa pre-determined intensity, measuring the fluorescence emission, determining the slope of a first regression line prior to full opening of the shutter; determining the slope of a second regression line of measurements after the shutter is fully opened and determining the intersecting point between these two lines. A method of determining the corrected and normalized fluorescence emissions from a plant is provided. 5017397 PROCESS FOR EXTRACTING ANTIOXIDANTS FROM LABIATAE HERBS
5,37
Method and apparatus for in vitro culture of cells in culture medium to produce a viable cell mass of cell number greater than the initial cell number, wherein a cell mass of particular cell number is cultured in a first oxygen-permeable culture sub-compartment to an increased cell number, and then aseptically transferred directly from the first sub-compartment to a next serial culture sub-compartment providing additional medium and/or culture space for further growth and reproduction to produce a viable cell mass of still higher cell number. Continued transfers are made, if necessary, to successive serial culture sub-compartment until a viable cell mass of desired cell number is attained, particularly one useful for seeding of a production-scale culture system.
5017491 Uy Nguyen, Grigory Frakman, David A Evans, Edmonton, Alberta, Canada Natural plant extracts exhibiting improved antioxidant properties are prepared from ground leaves of the Labiatae family of domestic herbs by application of a supercritical fluid extraction and fractionation process with carbon dioxide under specific operating conditions. The extracts have greater antioxidant activity than natural antioxidants extracted using other processes such as solvent extraction or molecular distillation. The extracts of the invention are oil soluble, colorless and flavorless when used at the optimum levels and provide more cost-effective protection from oxidation than existing natural antioxidants. They are effectve in animal and vegetable fats and oils, processed meats and fish, processed foods and beverages, food colorants, cosmetics and health-care products at usage reates of 0.0 I-0.05% of fat/oil. Starting materials include Rosmarinus spp. or Salvia spp. or Thymis spp. or Origanum spp. of the common domestic herbs rosemary, sage, thyme and oregano or residues of same after removal of volatile aromatic and flavor components by means of. steam distillation, subcritical carbon dioxide or supercritical carbon dioxide at pressures of less than 350 bar.
5017490 METHOD FOR IN VITRO REPRODUCTION AND GROWTH OF CELLS IN CULTURE MEDIUM Van Taiariol, Robert Oakley, Peter Ventura assigned to Baxter International Inc
PROCESS FOR REGENERATING S U N F L O W E R S BY EMBRYOGENESIS Georges Freyssinet, Martine Freyssinet, St Cyr Au Mont d'Or, France assigned to RhonePoulenc Agrochimie Process for regenerating cultivars from immature embryos. It comprises three stages: formation of embryogenic calluses in a medium containing a hormone of the cytokinin type, culturing the calluses in a medium of the same nature as above, culturing the plantlets. Application to sunflower cultivars.
5024837 COLEOPTERAN ACTIVE MICROORGANISMS, RELATED INSECTICIDE COMPOSITIONS AND METHODS FOR THEIR PRODUCTION AND USE William P Donovan, Jose M Gonzalez, Barry Levinson, Anthon Macaluso This invention relates to Bacillus thuringiensis strains that have insecticidal activity against lepidopteran and coleopteran insects, the coleopteran-active endotoxin being produced by an acquired plasmid. This invention also relates to the crystalline protein toxin useful as a biological insecticide against Coleoptera which toxin is produced by the strain of Bacillus thuringiensis. This invention also relates to the
5?,6
the viruses to human tissue, isolating, cultivating, and then processing the live mumps viruses. 5006336
germination is carried out in B5 or MS medium containing indolebutyric acid and gibberellic acid or MS medium containing filter sterilized Lproline and sucrose. Plantlet formation is carried out in B5 or MS medium without growth regulators. Plant and seed formation is carried out in soil or potting medium.
NOVEL COLEOPTERAN-ACTIVE BACILI,US THURINGIENSIS ISOLATE Jewel Payne assigned to Mycogen Corporation The subject invention concerns a novel and useful insecticide with activity against insect pests of the order Coleopters. Pests in the order Coleoptera do heavy damage to crops, e.g., corn and potatoes. The insecticide of the subject invention is a novel B. thuringiensis microbe referred to as B.t. PSI22D3, or mutants thereof. The spores or crystals of this microbe are useful to control coleopteran pests in various environments.
5008200 PROPAGATING MULTIPLE WHOLE FERTILE PLANTS FROM IMMATURE LEGUMINOUS
5012761 CHICKEN EGG HAVING RELATIVELY HIGH PERCENTAGE OF LONG CHAIN FATTY ACIDS AND METHOD OF REDUCING HEART RELATED D I S E A S E IN H U M A N S U S I N G SUCH EGGS Suk Y Oh Chickens are fed a composition including fish oil over a period of time. Modified eggs laid by such chickens contain substantial quantities of omega-3 polyunsaturated fatty acids. The modified eggs are fed to humans with the result that serum cholesterol, serum triglycerides and blood pressure are reduced as compared to humans eating a like number of regular eggs with a subsequent decrease in heart disease.
Jerome P Ranch, Julie Buchheim assigned to United AgriSeeds lnc Seeds of Glycine max, Glycine soja and hybrids thereof are produced by propagating embryogenic tissue containing multiple immature somatic embryos directly or serially by culturing particular cotyledonary tissue or derivation product thereof on media containing auxin selected from the group consisting of chloro-substituted phenoxyacetic acid, methyl derivative thereof, dicamba or picloram, incubating individual immature somatic embryos or embryogenic tissue containing such in a maturation stage to produce mature somatic embryos, germinating the mature somatic embryos to cause shoot tip formation, cultivating the germinated embryos to provide plantlets with 2 to 5 nodes and roots, cultivating the plantlets to provide whole fertile plants which flower and bear seeds, and recovering the seeds. Culturing to provide immature somatic embryos is carried out utilizing auxin in a concentration within the range of 2.5 to 1000 ppm effective to foster normal development of immature somatic embryos. 2,4dichlorophenoxyacetic acid is the preferred auxin. Maturation is carried out in B5 or MS medium containing indolebutyric acid and abscissic acid or activated charcoal and sucrose, and
5014225 APPARATUS AND METHOD FOR DETERMINING PLANT FLUORESCENCE William Vidaver, Peter Toivoncn. Sylvain Dube, West Vancouver, Canada assigned to Simon Fraser University An apparatus and method for determining the photosynthetic activity of a plant by determining the chlorophyll fluorescence of the plant is provided. The apparatus includes a light impermeable housing, a light to illuminate the housing, a light intensity in the housing and to adjust the light intensity controller. The light intensity may be between zero and 700 micromoles of photons per square meter per second. The monitor may be a photodiode protected by light filters which permit only light of wavelengths corresponding to plant fluorescence emission to pass to the photodiode. A computer analysis of the data obtained, corrects for Dark and Straylight signals in the housing and normalizes the data by correcting for the Fo fluoreseence. A
PATENT ABSTRACTS method of estimating Fo is provided which includes illuminating a light impermeable chamber housing a plant with light ofa pre-determined intensity, measuring the fluorescence emission, determining the slope of a first regression line prior to full opening of the shutter; determining the slope of a second regression line of measurements after the shutter is fully opened and determining the intersecting point between these two lines. A method of determining the corrected and normalized fluorescence emissions from a plant is provided. 5017397 PROCESS FOR EXTRACTING ANTIOXIDANTS FROM LABIATAE HERBS
5,37
Method and apparatus for in vitro culture of cells in culture medium to produce a viable cell mass of cell number greater than the initial cell number, wherein a cell mass of particular cell number is cultured in a first oxygen-permeable culture sub-compartment to an increased cell number, and then aseptically transferred directly from the first sub-compartment to a next serial culture sub-compartment providing additional medium and/or culture space for further growth and reproduction to produce a viable cell mass of still higher cell number. Continued transfers are made, if necessary, to successive serial culture sub-compartment until a viable cell mass of desired cell number is attained, particularly one useful for seeding of a production-scale culture system.
5017491 Uy Nguyen, Grigory Frakman, David A Evans, Edmonton, Alberta, Canada Natural plant extracts exhibiting improved antioxidant properties are prepared from ground leaves of the Labiatae family of domestic herbs by application of a supercritical fluid extraction and fractionation process with carbon dioxide under specific operating conditions. The extracts have greater antioxidant activity than natural antioxidants extracted using other processes such as solvent extraction or molecular distillation. The extracts of the invention are oil soluble, colorless and flavorless when used at the optimum levels and provide more cost-effective protection from oxidation than existing natural antioxidants. They are effectve in animal and vegetable fats and oils, processed meats and fish, processed foods and beverages, food colorants, cosmetics and health-care products at usage reates of 0.0 I-0.05% of fat/oil. Starting materials include Rosmarinus spp. or Salvia spp. or Thymis spp. or Origanum spp. of the common domestic herbs rosemary, sage, thyme and oregano or residues of same after removal of volatile aromatic and flavor components by means of. steam distillation, subcritical carbon dioxide or supercritical carbon dioxide at pressures of less than 350 bar.
5017490 METHOD FOR IN VITRO REPRODUCTION AND GROWTH OF CELLS IN CULTURE MEDIUM Van Taiariol, Robert Oakley, Peter Ventura assigned to Baxter International Inc
PROCESS FOR REGENERATING S U N F L O W E R S BY EMBRYOGENESIS Georges Freyssinet, Martine Freyssinet, St Cyr Au Mont d'Or, France assigned to RhonePoulenc Agrochimie Process for regenerating cultivars from immature embryos. It comprises three stages: formation of embryogenic calluses in a medium containing a hormone of the cytokinin type, culturing the calluses in a medium of the same nature as above, culturing the plantlets. Application to sunflower cultivars.
5024837 COLEOPTERAN ACTIVE MICROORGANISMS, RELATED INSECTICIDE COMPOSITIONS AND METHODS FOR THEIR PRODUCTION AND USE William P Donovan, Jose M Gonzalez, Barry Levinson, Anthon Macaluso This invention relates to Bacillus thuringiensis strains that have insecticidal activity against lepidopteran and coleopteran insects, the coleopteran-active endotoxin being produced by an acquired plasmid. This invention also relates to the crystalline protein toxin useful as a biological insecticide against Coleoptera which toxin is produced by the strain of Bacillus thuringiensis. This invention also relates to the