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5017493 DNA sequence
PATENT ABSTRACTS T lymphocytes. The serine esterase has an apparent molecular weight of approximately 28,000-31,000, as determined by SDS gel electrophoresis under reducing conditions, and trypsin-like activity. Inhibition of the esterase correlates with inhibition of the cells' cytolytic activity. Specific inhibition of the serine esterase is useful as a method for immunosuppression as well as for the inhibition of cytolytic activity ofT lymphocytes, both in vivo and in vitro• The genes encoding the routine and human serine esterase are homologous.
459
Disclosed and claimed are DNA gene segments, biologically functional plasmids and recombinant plasmids, and microorganism host cells containing such plasmids, all of which contain toluene monooxygenase genes from Pseudomonas mendocina KR-I and which are useful in a method for the microbial bioconversion of selected phenyl compounds to selected phenolic compounds. In particular, the method is useful for making p-hydroxyphenylacetic acid which is a valuable chemical intermediate in the preparation of certain antibiotics and certain betaadrcnergic blocking agents.
5017492 5017687 REVERSE TRANSCRIPTASE AND METHOD FOR ITS PRODUCTION Michael Kotewicz, James M D'Alessio, Gary F Gerard assigned to Life Technologies Inc The cloning of truncated versions of Moloney murine leukemia virus reverse transcriptase enzyme in E. coli is disclosed. The truncated reverse transcriptase enzymes have improved half-life when compared to native reverse transcriptase. 5017493 DNA SEQUENCE Henrik Andersen, Hans H M Dahl, Thorkild Christenscn, Charlottenlund, Denmark assigned to Nordisk Gentofte A/S A DNA sequence containing a ribosome binding site with a SD sequence AGGA and a start codon ATG, comprising a nucleotide sequence having the formula Y 1 ... YmZVAGGA X 1, X2 •.. XnATG wherein Y I . . . Ym represents a promoter with one or more restriction enzyme sites. m being an integer corresponding to the number of base pairs in the promoter, each X, Y, Z and V is A, T, C or G; n is 9 to 13, and the sequence X I . •. Xn cannot contain a start codon.
PEPTIDES FOR THE DETECTION OF HTLV-I INFECTION Anders Vahlne, Bo Svennerholm, Lars Rymo, Stig Jeansson, Peter Horal. Hovas, Sweden assigned to Virovahl S A Four peptides corresponding to regions of the glycoprotein encoded by the env gene of HTLV I are provided. These peptides which are immunologically reactive with HTLV-I specific antibodies are useful in assays for detection of HTLV-I infection or exposure and in compositions to elicit the production of antibodies against HTLV-I in animals and man.
5017692 TRUNCATED HUMAN INTERLEUKIN-A ALPHA Gerar Zurawski, Sandra M Zurawski assigned to Schering Corporation Synthetic human interleukin-I alpha genes are provided whose codons are selected from those preferred by bacteria. High expression levels are obtained in an E. eoli expression system for both native interleukin-I alpha and several mutant interleukin-I alpha's.
5017495
5018209 PLASMID ENCODING THE PSEUDOMONAS MENDOCINA TOLUENE MONOOXYGENASE GENE Kwang-Mu Yen, Lawrence M Blatt assigned to Amgen lnc
ANALYSIS METHOD AND APPARATUS FOR BIOLOGICAL SPECIMENS James Bacus assigned to Cell Analysis Systems lnc
459
Disclosed and claimed are DNA gene segments, biologically functional plasmids and recombinant plasmids, and microorganism host cells containing such plasmids, all of which contain toluene monooxygenase genes from Pseudomonas mendocina KR-I and which are useful in a method for the microbial bioconversion of selected phenyl compounds to selected phenolic compounds. In particular, the method is useful for making p-hydroxyphenylacetic acid which is a valuable chemical intermediate in the preparation of certain antibiotics and certain betaadrcnergic blocking agents.
5017492 5017687 REVERSE TRANSCRIPTASE AND METHOD FOR ITS PRODUCTION Michael Kotewicz, James M D'Alessio, Gary F Gerard assigned to Life Technologies Inc The cloning of truncated versions of Moloney murine leukemia virus reverse transcriptase enzyme in E. coli is disclosed. The truncated reverse transcriptase enzymes have improved half-life when compared to native reverse transcriptase. 5017493 DNA SEQUENCE Henrik Andersen, Hans H M Dahl, Thorkild Christenscn, Charlottenlund, Denmark assigned to Nordisk Gentofte A/S A DNA sequence containing a ribosome binding site with a SD sequence AGGA and a start codon ATG, comprising a nucleotide sequence having the formula Y 1 ... YmZVAGGA X 1, X2 •.. XnATG wherein Y I . . . Ym represents a promoter with one or more restriction enzyme sites. m being an integer corresponding to the number of base pairs in the promoter, each X, Y, Z and V is A, T, C or G; n is 9 to 13, and the sequence X I . •. Xn cannot contain a start codon.
PEPTIDES FOR THE DETECTION OF HTLV-I INFECTION Anders Vahlne, Bo Svennerholm, Lars Rymo, Stig Jeansson, Peter Horal. Hovas, Sweden assigned to Virovahl S A Four peptides corresponding to regions of the glycoprotein encoded by the env gene of HTLV I are provided. These peptides which are immunologically reactive with HTLV-I specific antibodies are useful in assays for detection of HTLV-I infection or exposure and in compositions to elicit the production of antibodies against HTLV-I in animals and man.
5017692 TRUNCATED HUMAN INTERLEUKIN-A ALPHA Gerar Zurawski, Sandra M Zurawski assigned to Schering Corporation Synthetic human interleukin-I alpha genes are provided whose codons are selected from those preferred by bacteria. High expression levels are obtained in an E. eoli expression system for both native interleukin-I alpha and several mutant interleukin-I alpha's.
5017495
5018209 PLASMID ENCODING THE PSEUDOMONAS MENDOCINA TOLUENE MONOOXYGENASE GENE Kwang-Mu Yen, Lawrence M Blatt assigned to Amgen lnc
ANALYSIS METHOD AND APPARATUS FOR BIOLOGICAL SPECIMENS James Bacus assigned to Cell Analysis Systems lnc