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5028540 Avian immunoglobulin producing cell lines
PATENT ABSTRACTS
538
expression in various microorganisms of the gene, known as cryC, which codes for this toxin, and for related novel insecticide compositions and methods for their use.
5024944 TRANSFORMATION, SOMATIC EMBRYOGENESIS AND WHOLE PLANT REGENERATION METHOD FOR GLYCINE SPECIES Glenn B Collins, David F Hildebrand, Paul Lazzeri, Thomas Adams, Wayne A Parrott, Lynn M Hartweck assigned to Lubrizol Genetics lnc; The University of Kentucky Research Foundation Inc A method for somatic embryogcnesis of soybean, (Glycine max), Glycine soja and other Glycine species is provided using immature cotyledon tissue, preferably with the embryonic axis removed, comprising culturing said tissue on a medium containing auxin, preferably NAA at a concentration of at least about 15 mg/I. A further method for such somatic embryogenesis is provided wherein the culture medium contains a synergistically acting lowered carbohydrate and auxin concentration. Particularly embryogenic cells of such tissue are identified and improved maceration methods for contacting such cells with regeneration and transformation media are disclosed. Methods for transforming somatic tissue from soybean and other Glycine species are also provided. Whole, fertile, transformed plants are obtained.
5026547 COMPOUND HAVING INSECTICIDAL ACTIVITY AND INSECTICIDE COMPOSITION CONTAINING THE SAME Kei-ichi Imamura, Shuichi Gomi, Michiaki lwata, Shinji Miyadoh, Masaru Shimura, Takashi Shomura, Masaji Sezaki, Shigeharu Inoye, Kanagawa, Japan assigned to Meiji Seika Kaisha Ltd A novel compound having an intense insecticidal activity and a process for producing the same which comprises culturing a strain belonging to the genus Humicola and isolating the compound from the culture.
5028253 PROCESS FOR CONTROLLING UNDESIRABLE VEGETATION WITH COLLETOTRICHUM COCCODES SPORES Alan K Watson, Alan R Gotlieb, Pincourt, VT, Canada assigned to The Royal Institution for the Advancement of Learning (McGill Univ ); The Univ of Vermont and State Agriculturual Colleg There is disclosed a process for controlling undesirable vegetation, especially velvetleaf by applying Colletotrichum coccodes spores onto the undesirable vegetation or onto the situs of the undesirable vegetation. The composition including the fungus spores in an agricultural carrier as well as the growing of the fungus on solid or liquid media are also disclosed. The spores germinate, infect the undesirable vegetation and result in a pathogenic disease which kills or reduces the growth of this undesirable plant.
5028540 AVIAN IMMUNOGLOBULIN PRODUCING CELL LINES Eric H Humphries assigned to The Board of Regents The University of Texas System The present invention involves a method for preparing antibody-producing chicken cell clones. This method comprises a series of steps including initially immunizing a first chicken with a desired antigen. A population of antibody-producing lymphocytes from bursa or spleen of the first chicken is separated. The antibody-producing lymphocyte population is then infected with helper-free reticuloendotheliosis virus REV-T or helper-free reticuloendotheliosis virus REV-T and CSV (preferably REV-T(CSV) and transplanted into a second chicken, the second chicken having been pretreated to remove normal B cells. The transplanted lymphocytes to proliferate in the . ~ o n d chicken, preferably for a period of at least about two weeks. The lymphocytes from spleen, bursa or peripheral blood of the second chicken are isolated and plated out, for example in microtiter plates. Cell clones producing antibody such as IgG or IgM directed against the desired antigen are then selected. The isolation step most preferably involves cell culturing in the microtitcr plates for a period of at least about one week.
538
expression in various microorganisms of the gene, known as cryC, which codes for this toxin, and for related novel insecticide compositions and methods for their use.
5024944 TRANSFORMATION, SOMATIC EMBRYOGENESIS AND WHOLE PLANT REGENERATION METHOD FOR GLYCINE SPECIES Glenn B Collins, David F Hildebrand, Paul Lazzeri, Thomas Adams, Wayne A Parrott, Lynn M Hartweck assigned to Lubrizol Genetics lnc; The University of Kentucky Research Foundation Inc A method for somatic embryogcnesis of soybean, (Glycine max), Glycine soja and other Glycine species is provided using immature cotyledon tissue, preferably with the embryonic axis removed, comprising culturing said tissue on a medium containing auxin, preferably NAA at a concentration of at least about 15 mg/I. A further method for such somatic embryogenesis is provided wherein the culture medium contains a synergistically acting lowered carbohydrate and auxin concentration. Particularly embryogenic cells of such tissue are identified and improved maceration methods for contacting such cells with regeneration and transformation media are disclosed. Methods for transforming somatic tissue from soybean and other Glycine species are also provided. Whole, fertile, transformed plants are obtained.
5026547 COMPOUND HAVING INSECTICIDAL ACTIVITY AND INSECTICIDE COMPOSITION CONTAINING THE SAME Kei-ichi Imamura, Shuichi Gomi, Michiaki lwata, Shinji Miyadoh, Masaru Shimura, Takashi Shomura, Masaji Sezaki, Shigeharu Inoye, Kanagawa, Japan assigned to Meiji Seika Kaisha Ltd A novel compound having an intense insecticidal activity and a process for producing the same which comprises culturing a strain belonging to the genus Humicola and isolating the compound from the culture.
5028253 PROCESS FOR CONTROLLING UNDESIRABLE VEGETATION WITH COLLETOTRICHUM COCCODES SPORES Alan K Watson, Alan R Gotlieb, Pincourt, VT, Canada assigned to The Royal Institution for the Advancement of Learning (McGill Univ ); The Univ of Vermont and State Agriculturual Colleg There is disclosed a process for controlling undesirable vegetation, especially velvetleaf by applying Colletotrichum coccodes spores onto the undesirable vegetation or onto the situs of the undesirable vegetation. The composition including the fungus spores in an agricultural carrier as well as the growing of the fungus on solid or liquid media are also disclosed. The spores germinate, infect the undesirable vegetation and result in a pathogenic disease which kills or reduces the growth of this undesirable plant.
5028540 AVIAN IMMUNOGLOBULIN PRODUCING CELL LINES Eric H Humphries assigned to The Board of Regents The University of Texas System The present invention involves a method for preparing antibody-producing chicken cell clones. This method comprises a series of steps including initially immunizing a first chicken with a desired antigen. A population of antibody-producing lymphocytes from bursa or spleen of the first chicken is separated. The antibody-producing lymphocyte population is then infected with helper-free reticuloendotheliosis virus REV-T or helper-free reticuloendotheliosis virus REV-T and CSV (preferably REV-T(CSV) and transplanted into a second chicken, the second chicken having been pretreated to remove normal B cells. The transplanted lymphocytes to proliferate in the . ~ o n d chicken, preferably for a period of at least about two weeks. The lymphocytes from spleen, bursa or peripheral blood of the second chicken are isolated and plated out, for example in microtiter plates. Cell clones producing antibody such as IgG or IgM directed against the desired antigen are then selected. The isolation step most preferably involves cell culturing in the microtitcr plates for a period of at least about one week.