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5036002 Expression system with trans-acting DNA segments
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I'AI FNI ABSTRACTS 5t)35996
5036011
PROCESS FOR CONTROLLING CONTAMINATION OF NUCLEIC ACID AMPI,IFICATION REACTIONS
N O V E L A U R E O B A S I D I U M SP. MICROORGANISMS AND METHOD FOR OBTAINING THE SAME, AND METHOD FOR PREPARING ERYTHRITOL WITH THE SAME
James L Hartley assigned to 1,ife q'echnologies 1no In the process according to this invention, an amplification procedure is performed on a first sample in which one or more of the four normal ribonucleoside triphosphates (rNTPs) or deoxyribonucleoside triphosphates (dNTPs) is replaced with an exo-sample nucleotide. After amplification, any contaminating amplified product that may be remaining is subjected to a physical, chemical, enzymatic, or biological treatment which renders nucleic acid containing the exo-sample nucleotide substantially unamplifiable. The treatment may be done as a separate step or it may be done in the presence of a second sample containing nucleic acid sequences to be amplified. The amplified nucleic acid sequences derived from the first sample which contaminate the second sample are not further substantially ampfified during amplification of nucleic acid sequences ot" the second sample.
Takash Sasaki, Takafumi Kasumi, Naoya Kubo, Keiji Kainuma, Katsuo Wako, Hiroaki lshizuka, Gaku Kawaguchi, Tsunero Oda, Ibaraki, Japan assigned to Director of National Food Research Institute Ministry of Agriculture Forestry and Fisheries: Nikkcn Chemicals Co Ltd
5036002
Biologically pure culture of Aureobasidium sp. SN-124A strain (FERM BP-1429) and artificial mutants thereof according to the present invention show the properties of forming and accumulating er3,'thritol in a cuhurc solution, when aerobically cultured on a liquid culture medium containing an assimilable carbon source and an assimilable nitrogen source, and are useful for the preparation of erythritol by fermentation of sugars. A method for preparing erythritol by fermentation of sugars according to the present invention comprises inoculating microorganism selected from the group consisting of Aureobasidium sp. SN-124A strain (FERM BP1429) and mutants thereof on a liquid culture medium of pH 4 to 9 containing an assimilable carbon source and an assimilable nitrogen source, and aerobically culturing them at a temperature of 30 degrees to 38 degrees C. to form and accumulate erythritol in said culture medium for collection.
EXPRESSION SYSTEM WITH TRANS-ACTING DNA SEGMENTS
5043262
Sven Hastrup, Copenhagen. Denmark assigned to Novo-Nordisk A/S
PROTEIN, SEQUENCES CONTAINING THE VPU GENE THEREFORE, VECTORS,
Gene expression systems comprising an expression vector and a trans-acting DNA segment , where the expression vector comprises the gene or genes to be expressed and one or more cisacting regulatory elements which are responsive to a trans-acting factor produced by said transacting DNA segment . More specifically the invention relates to such gene expression systems where said trans-acting DNA segment and said cis-acting regulatory elements comprise one or more segments of the genome from a Bacillus species. Methods for stimulating the production of gene products, vectors for transforming microorganisms, and their use are also disclosed.
M E T H O D S OF PREPARATION AND USE William A Hasehine, Ernes Terwilliger, Eri Cohen assigned to Dana Farber Cancer Institute A protein having molecular weight of approximately 16 kD which is also cleaved into a protein having a molecular weight of 15 kD is disclosed. This protein is referred to as viral protein [J and produced by the vpu gene. It is disclosed that this protein has antigenic determinants and can be used to screen for people having the HIV-I virus.
I'AI FNI ABSTRACTS 5t)35996
5036011
PROCESS FOR CONTROLLING CONTAMINATION OF NUCLEIC ACID AMPI,IFICATION REACTIONS
N O V E L A U R E O B A S I D I U M SP. MICROORGANISMS AND METHOD FOR OBTAINING THE SAME, AND METHOD FOR PREPARING ERYTHRITOL WITH THE SAME
James L Hartley assigned to 1,ife q'echnologies 1no In the process according to this invention, an amplification procedure is performed on a first sample in which one or more of the four normal ribonucleoside triphosphates (rNTPs) or deoxyribonucleoside triphosphates (dNTPs) is replaced with an exo-sample nucleotide. After amplification, any contaminating amplified product that may be remaining is subjected to a physical, chemical, enzymatic, or biological treatment which renders nucleic acid containing the exo-sample nucleotide substantially unamplifiable. The treatment may be done as a separate step or it may be done in the presence of a second sample containing nucleic acid sequences to be amplified. The amplified nucleic acid sequences derived from the first sample which contaminate the second sample are not further substantially ampfified during amplification of nucleic acid sequences ot" the second sample.
Takash Sasaki, Takafumi Kasumi, Naoya Kubo, Keiji Kainuma, Katsuo Wako, Hiroaki lshizuka, Gaku Kawaguchi, Tsunero Oda, Ibaraki, Japan assigned to Director of National Food Research Institute Ministry of Agriculture Forestry and Fisheries: Nikkcn Chemicals Co Ltd
5036002
Biologically pure culture of Aureobasidium sp. SN-124A strain (FERM BP-1429) and artificial mutants thereof according to the present invention show the properties of forming and accumulating er3,'thritol in a cuhurc solution, when aerobically cultured on a liquid culture medium containing an assimilable carbon source and an assimilable nitrogen source, and are useful for the preparation of erythritol by fermentation of sugars. A method for preparing erythritol by fermentation of sugars according to the present invention comprises inoculating microorganism selected from the group consisting of Aureobasidium sp. SN-124A strain (FERM BP1429) and mutants thereof on a liquid culture medium of pH 4 to 9 containing an assimilable carbon source and an assimilable nitrogen source, and aerobically culturing them at a temperature of 30 degrees to 38 degrees C. to form and accumulate erythritol in said culture medium for collection.
EXPRESSION SYSTEM WITH TRANS-ACTING DNA SEGMENTS
5043262
Sven Hastrup, Copenhagen. Denmark assigned to Novo-Nordisk A/S
PROTEIN, SEQUENCES CONTAINING THE VPU GENE THEREFORE, VECTORS,
Gene expression systems comprising an expression vector and a trans-acting DNA segment , where the expression vector comprises the gene or genes to be expressed and one or more cisacting regulatory elements which are responsive to a trans-acting factor produced by said transacting DNA segment . More specifically the invention relates to such gene expression systems where said trans-acting DNA segment and said cis-acting regulatory elements comprise one or more segments of the genome from a Bacillus species. Methods for stimulating the production of gene products, vectors for transforming microorganisms, and their use are also disclosed.
M E T H O D S OF PREPARATION AND USE William A Hasehine, Ernes Terwilliger, Eri Cohen assigned to Dana Farber Cancer Institute A protein having molecular weight of approximately 16 kD which is also cleaved into a protein having a molecular weight of 15 kD is disclosed. This protein is referred to as viral protein [J and produced by the vpu gene. It is disclosed that this protein has antigenic determinants and can be used to screen for people having the HIV-I virus.