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5045450 Determination of a mutational spectrum
New Patents
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sequence. Differences in the base sequence of the test DNA indicate an increased likelihood that the person will develop vascular aneurysm. Family members of the mammal tested can also be tested to determine if they also have a genetic predisposition to vascular aneurysm by testing for the presence of the mutation in the pro alphal(II1) gene of the family members.
5045450 DETERMINATION OF A MUTATIONAL SPECTRUM William G Thilly, Phouthone Keohavong assigned to Massachusetts Institute of Technology A method of resolving (physically separating) mutant DNA from nonmutant DNA and a method of defining or establishing a mutational spectrum or profile of alterations present in nucleic acid sequences from a sample to be analyzed, such as a tissue or body fluid. The present method is based on the fact that it is possible, through the use of DGGE, to separate nucleic acid sequences which differ by only a single base change and on the ability to detect the separate mutant molecules. The present invention, in another aspect, relates to a method for determining a mutational spectrum in a DNA sequence of interest present in a population of cells. The method of the present invention is useful as a diagnostic or analytical tool in forensic science in assessing environmental and/or occupational exposures to potentially genetically toxic materials (also referred to as potential mutagens); in biotechnology, particularly in the study of the relationship between the amino acid sequence of enzymes and other biologicallyactive proteins or protein-containing substances and their respective functions; and in determining the effects of drugs, cosmetics and other chemicals for which toxicity data must be obtained.
5045455 FACTOR VIII:C CDNA CLONING AND EXPRESSION Georg Kuo, Frank Masiarz, Martha Truett, Pablo Valenzuela, Mirella E Rasmussen, Jennifer M Favaloro, Daniel Caput, Rae L Burke, Carol Pachl assigned to Chiron Corporation Methods and compositions are provided for recombinant DNA production of Factor VIIIC
and truncated derivatives thereof. Based on amino acid sequences, probes are developed for isolating messenger RNA, cDNA and/or chromosomal DNA encoding for Factor VIIIC. The Factor VIIIC gene in its entirety, a fragment thereof, or a cDNA is then used for expression of Factor VIIIC in a host. The bacteriophage lambdaFVIII23D containing the 14.43kb EcoRI fragment was deposited at the A.T.C.C. on Jan. 4,1984 and given Accession No. 40094. Also, the vector pSVF8-200 was deposited at the A.T.C.C. on July 17, 1985 and given Accession No. 40190.
5045460 DNA SEQUENCE ENCODING METALLOCARBOXYPEPTIDASE INHIBITOR PROTEIN Belinda M Martineau, Kevin McBride assigned to Calgene Inc In accordance with the subject invention, novel DNA sequences, heterologous constructs and plant expression vectors are provided which relate to the metallocarboxypeptidase inhibitor (MCPI) protein. MCPI protein is capable of inactivating metallocarboxypeptidase, exopeptidase-type digestive enzymes. In addition, this application relates to plant cells and plant entities, including whole plants, plant seed, and plant parts, containing such constructs and methods of providing transgenic plants capable of inhibiting metallocarboxypeptidase (MCP) proteinases and methods of using such for the tolerence of MCPI sensitive pests.
5045461 METHOD FOR INCREASING YIELD AND NODULATION BY BRADYRHIZOBIUM Kieran Scott, Waverly, Australia Lubrizol Genetics Inc
assigned to
A method of increasing nodulation of a plant capable of being nodulated by Bradyrhizobium sp. (Parasponis) is provided comprising infecting said plant with a Bradyrhizobium sp. (Parasponia) species mutated such that nodK is nonfunctional. One such method of mutation involves insertion upstream of nodABC constitutive promoter sequences capable of activating nodABC. The invention is exemplified by the inoculation of siratro with mutated Bradyrhizobium sp. (Parasponia) mutated by in-
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sequence. Differences in the base sequence of the test DNA indicate an increased likelihood that the person will develop vascular aneurysm. Family members of the mammal tested can also be tested to determine if they also have a genetic predisposition to vascular aneurysm by testing for the presence of the mutation in the pro alphal(II1) gene of the family members.
5045450 DETERMINATION OF A MUTATIONAL SPECTRUM William G Thilly, Phouthone Keohavong assigned to Massachusetts Institute of Technology A method of resolving (physically separating) mutant DNA from nonmutant DNA and a method of defining or establishing a mutational spectrum or profile of alterations present in nucleic acid sequences from a sample to be analyzed, such as a tissue or body fluid. The present method is based on the fact that it is possible, through the use of DGGE, to separate nucleic acid sequences which differ by only a single base change and on the ability to detect the separate mutant molecules. The present invention, in another aspect, relates to a method for determining a mutational spectrum in a DNA sequence of interest present in a population of cells. The method of the present invention is useful as a diagnostic or analytical tool in forensic science in assessing environmental and/or occupational exposures to potentially genetically toxic materials (also referred to as potential mutagens); in biotechnology, particularly in the study of the relationship between the amino acid sequence of enzymes and other biologicallyactive proteins or protein-containing substances and their respective functions; and in determining the effects of drugs, cosmetics and other chemicals for which toxicity data must be obtained.
5045455 FACTOR VIII:C CDNA CLONING AND EXPRESSION Georg Kuo, Frank Masiarz, Martha Truett, Pablo Valenzuela, Mirella E Rasmussen, Jennifer M Favaloro, Daniel Caput, Rae L Burke, Carol Pachl assigned to Chiron Corporation Methods and compositions are provided for recombinant DNA production of Factor VIIIC
and truncated derivatives thereof. Based on amino acid sequences, probes are developed for isolating messenger RNA, cDNA and/or chromosomal DNA encoding for Factor VIIIC. The Factor VIIIC gene in its entirety, a fragment thereof, or a cDNA is then used for expression of Factor VIIIC in a host. The bacteriophage lambdaFVIII23D containing the 14.43kb EcoRI fragment was deposited at the A.T.C.C. on Jan. 4,1984 and given Accession No. 40094. Also, the vector pSVF8-200 was deposited at the A.T.C.C. on July 17, 1985 and given Accession No. 40190.
5045460 DNA SEQUENCE ENCODING METALLOCARBOXYPEPTIDASE INHIBITOR PROTEIN Belinda M Martineau, Kevin McBride assigned to Calgene Inc In accordance with the subject invention, novel DNA sequences, heterologous constructs and plant expression vectors are provided which relate to the metallocarboxypeptidase inhibitor (MCPI) protein. MCPI protein is capable of inactivating metallocarboxypeptidase, exopeptidase-type digestive enzymes. In addition, this application relates to plant cells and plant entities, including whole plants, plant seed, and plant parts, containing such constructs and methods of providing transgenic plants capable of inhibiting metallocarboxypeptidase (MCP) proteinases and methods of using such for the tolerence of MCPI sensitive pests.
5045461 METHOD FOR INCREASING YIELD AND NODULATION BY BRADYRHIZOBIUM Kieran Scott, Waverly, Australia Lubrizol Genetics Inc
assigned to
A method of increasing nodulation of a plant capable of being nodulated by Bradyrhizobium sp. (Parasponis) is provided comprising infecting said plant with a Bradyrhizobium sp. (Parasponia) species mutated such that nodK is nonfunctional. One such method of mutation involves insertion upstream of nodABC constitutive promoter sequences capable of activating nodABC. The invention is exemplified by the inoculation of siratro with mutated Bradyrhizobium sp. (Parasponia) mutated by in-